Gokcek, Oznur BeguemBas, FurkanMuratcobanoglu, HamdiDemirel, Sevgi2024-11-072024-11-0720230016-23611873-7153https://doi.org/10.1016/j.fuel.2023.127475https://hdl.handle.net/11480/14011Along with advancing technologies, the interest in producing renewable hydrogen by biological methods has recently increased due to its economic and eco-friendly properties. Various pretreatment methods or additives, such as enzymes and conductive materials, applied to increase the efficiency of biohydrogen production have been recently studied. In the current research, magnetite was utilized to increase the bioactivity of hydrogenproducing microorganisms during dark fermentation (DF). In the study, reactors with a S/I ratio of 2 gVS/gVS were set up, and magnetite was added at concentrations of 0, 50, 100, and 200 mg/L. The maximum biohydrogen production of 73.59 ml/gVS was obtained at 100 mg/L magnetite addition. Biohydrogen production increased by 46 % with 100 mg/L magnetite addition. In the magnetite-supported reactor, the dominant bacterial genera were Sporolactobacillus (39 %), Clostridium (15 %), and Coprothermobacter (10 %). It was thought that the change in microbial distribution positively affected biohydrogen production with the addition of magnetite.eninfo:eu-repo/semantics/closedAccessApple pulp wasteBiohydrogen productionMagnetite nanoparticuleConductive materialDark fermentationArticle33910.1016/j.fuel.2023.1274752-s2.0-85146182455Q1WOS:000919890900001Q1