Yavuz, CanerCaliskan, Mehmet Emin2024-11-072024-11-0720220133-37201788-9170https://doi.org/10.1007/s42976-021-00210-9https://hdl.handle.net/11480/14194Recalcitrant plants are difficult to handle in tissue culture, and this limits their accessibility for various studies. Novel strategies are being developed to compensate the restricted nature of these plants to make them more adapted to tissue culture applications. Sorghum is one of the most recalcitrant plants with decreased efficiency and non-standardized tissue culture protocols. The advances in embryogenic genes ease the somatic embryo formation and regeneration ability in sorghum. BABY BOOM (BBM) is the most prominent transcription factor involved in somatic embryogenesis, even without exogenous auxin application, the spontaneous somatic embryo formation could occur in plants, which makes this gene(s) an optimal target for recalcitrant plants. This study is designed to characterize SbBBM-like genes in sorghum. Two similar length of protein pairs, SbBBM-like 1 (XP_021313568.1) and SbBBM-like 2 (XP_002452443.1), were identified in cv. Aldari and cis-regulatory elements of these proteins were observed to be involved in particularly light response and hormonal regulation. The protein -protein interaction data indicated possible role in auxin mechanism. The expression data showed SbBBM-like was highly expressed in seedling root and embryogenic callus, however the expression was highest in embryogenic callus for SbBBM-like 2 with almost 200-fold increase, compared to other tissues.eninfo:eu-repo/semantics/closedAccessSorghumSomatic embryogenesisBABY BOOMIn silicoArticle50466767610.1007/s42976-021-00210-92-s2.0-85115238282Q2WOS:000698071800001Q3