Ertas, N.Gonulalan, Z.Yildirim, Y.Karadal, F.Abay, S.Al, S.2019-08-012019-08-0120130266-82541472-765Xhttps://dx.doi.org/10.1111/lam.12124https://hdl.handle.net/11480/4349The present study was conducted to investigate the presence of Escherichia coli O157:H7 in food samples of animal origin and to detect its virulence genes by immunomagnetic separation technique and multiplex PCR (mPCR). A total of 500 samples (consisting of diced meat, minced meat, burger, raw cow's milk and raw cow's milk cheese) were analysed. Escherichia coli O157:H7 was detected in 5 (1%) of 500 analysed samples including two diced meat, one minced meat and two raw-milk cheese. None of the burger samples tested contained E.coli O157:H7. Three isolates obtained from minced and diced meat were found to carry stx(1), stx(2), hlyA and eaeA genes whereas two isolates from raw-milk cheese were found to harbour the stx(1), eaeA and hlyA genes. The results of this study suggest that raw meat and raw-milk cheese tested could pose public health problems in consumers with regard to their virulence factors.eninfo:eu-repo/semantics/openAccessEscherichia coli O157:H7foods of animal originimmunomagnetic separationmPCRvirulence genesArticle57437337910.1111/lam.12124238090612-s2.0-84884703864Q3WOS:000325034500018Q3