Şahinöz, ErdinçDoğu, ZaferAral, Faruk2024-11-072024-11-0720182148-127Xhttps://doi.org/10.24925/turjaf.v6i3.380-386.1766https://search.trdizin.gov.tr/tr/yayin/detay/323380https://hdl.handle.net/11480/12277This study is performed to determine some of sperm quality after applying freezing /thawing process. Thus, the aim of this study is to examine different cryprotective agentswith additives in terms of their effects at different pH on the cryopreservation process oflongspine scraper (Capoeta trutta). The present study, twelve media were prepared bymixing three different cryoprotectants (dimethyl sulfoxide (DMSO, (CH3)2SO); methanol(CH3OH); methyl glycol (MG, CH3O (CH2)2OH)) with an extenders (glucose) at fourdifferent pH (7.2, 7.6, 8.0 and 8.4) for longspine scraper semen. Considering the findingsfrom the examination (The motility rate after thawing process and duration of motilityobtained in DMSO as 81% and 20 min, in methanol as 73% and 12 min, in methyl glycolas 60% and 15 min.), we can conclude that the DMSO is the best freezing media in orderto create new essays in cryopreservation for sperm of Capoeta trutta in the future.eninfo:eu-repo/semantics/openAccessBiyolojiZoolojiArticle6338038610.24925/turjaf.v6i3.380-386.1766323380