Yıldırım, ZelihaSakin, TubaÇoban, Fatma2024-11-072024-11-0720182148-127Xhttps://doi.org/10.24925/turjaf.v6i9.1200-1208.2000https://search.trdizin.gov.tr/tr/yayin/detay/329173https://hdl.handle.net/11480/12284The aim of this study was to isolate, purify and determine host ranges of bacteriophagesinfecting Escherichia coli O157:H7 from different environment such as river/streamwater, sewage, raw food, animal troughs, wastewaters of food processing plants,slaughterhouse and fish farms. For screening of E. coli O157:H7 bacteriophages, 92samples were used. It was found that in respect to anti-E. coli O157:H7 bacteriophages,food processing wastewaters, sewage and slaughterhouse wastewaters are the richestsources, and streams, troughs and fisheries wastewater are rich in the middle, and rawfoods were the poorest source. A total of 37 phages were isolated and purified. Thephages counts of the purified samples were changed among 30×103 - 34×108 PFU/mL.The isolated phages were generally infective against E. coli O157:H7 and E. coli strainsand 81.08% of the phages (30 out of 37) formed clear plaques and were capable to lyse atleast 1 out of 5 E. coli O157:H7 strains. In addition to E. coli, some phages were capableto infect some Salmonella enterica serovars. This results show that inhibitor spectra ofthe phages were wide.trinfo:eu-repo/semantics/openAccessMikrobiyolojiBiyoteknoloji ve Uygulamalı MikrobiyolojiVirolojiIsolation of Anti-Escherichia coli O157:H7 Bacteriophages and Determination of Their Host RangesArticle691200120810.24925/turjaf.v6i9.1200-1208.2000329173