Tan, GamzeIlk, SedefFoto, Fatma Z.Foto, EgemenSaglam, Necdet2024-11-072024-11-0720212287-237X2287-2388https://doi.org/10.12989/anr.2021.10.2.139https://hdl.handle.net/11480/16350In this study, phytochemicals present in Propolis Extract (PE) were employed as reducing and stabilizing reagents to synthesize silver nanoparticles. Three propolis-reduced silver nanoparticles (P-AgNPs1-3) were synthesized using increasing amounts of PE. P-AgNPs were treated with different cancer cells -lung (A549), cervix (HeLa) and colon (WiDr) for 24, 48 and 72 h to evaluate their anti -proliferative activities. A non-cancerous cell type (L929) was also used to test whether suppressive effects of P-AgNPs on cancer cell proliferation were due to a general cytotoxic effect. The characterization results showed that the bioactive contents in propolis successfully induced particle formation. As the amount of PE increased, the particle size decreased; however, the size distribution range expanded. The antioxidant capacity of the particles increased with increased propolis amounts. P-AgNP1 exhibited almost equal inhibitory effects across all cancer cell types; however, P-AgNP2 was more effective on HeLa cells. P-AgNPs3 showed greater inhibitory effects in almost all cancer cells compared to other NPs and pure propolis. Consequently, the biological effects of P-AgNPs were highly dependent on PE amount, NP concentration, and cell type. These results suggest that AgNPs synthesized utilizing propolis phytochemicals might serve as anti -cancer agents, providing greater efficacy against cancer cells.eninfo:eu-repo/semantics/closedAccesspropolissilver nanoparticlesDPPHSRB assayanti -cancer activitycytotoxicityAntioxidative and antiproliferative effects of propolis-reduced silver nanoparticlesArticle10213915010.12989/anr.2021.10.2.1392-s2.0-85105822367Q1WOS:000643257800004Q1