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    Occurence and antimicrobial resistance of Staphylococcus aureus and Salmonella spp. in retail fish samples in Turkey
    (PERGAMON-ELSEVIER SCIENCE LTD, 2015) Onmaz, Nurhan Ertas; Abay, Secil; Karadal, Fulden; Hizlisoy, Harun; Telli, Nihat; Al, Serhat
    The aims of this study were to investigate the presence of Staphylococcus aureus and staphylococcal enterotoxins, as well as Salmonella spp. and to determine the antimicrobial susceptibilities of the isolates from fish samples. A total of 100 fish samples were analysed consisting of 30 anchovy, 35 trout and 35 sea bream. The presence of SEs was detected using ELISA and its genes confirmed by mPCR. Also, S. aureus and Salmonella spp. were detected in 9 (9%) and 5 (5%) samples, respectively. None of the S. aureus isolates had SEs and SEs genes. The resistance rates of the S. aureus isolates to erythromycin, tetracycline, and penicillin G were found to be 33% while Salmonella spp. isolates were resistant to trimethoprim-sulfamethoxazole, gentamicin and neomycine in 20%, 20% and 80%, respectively of the samples. It is of utmost important for public health that retail fish markets need to use hygienic practices in handling and processing operations. (C) 2014 Elsevier Ltd. All rights reserved.
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    PREVALENCE OF ESCHERICHIA COLI O157: H7 AND THEIR VEROTOXINS AND SALMONELLA SPP. IN PROCESSED POULTRY PRODUCTS
    (WILEY-BLACKWELL, 2013) Karadal, Fulden; Ertas, Nurhan; Hizlisoy, Harun; Abay, Secil; Al, Serhat
    This study was carried out to investigate the presence of Escherichia coliO157:H7 and their verotoxins as well as Salmonella spp. in processed poultry products. A total of 100 samples of cordon bleus (n=20), chicken nuggets (n=20), chicken burgers (n=40) and chicken meatballs (n=20) were analyzed. The E.coliO157 (but not H7) strain was isolated from one (1%) out of 100 samples. This isolate were obtained from chicken burgers. Multiplex polymerase chain reaction confirmed two genotypes, namely rfbO157 and stx1 genes. None of the samples was contaminated with Salmonella spp. These results demonstrate that processed poultry products may be sporadically contaminated with E.coliO157 strains that are potentially pathogenic for humans. Practical ApplicationThe growth of E.coliO157 and Salmonella in processed poultry products are dependent on poultry carcasses used as raw material, hygienic application during the process and the storage temperature. Potentially pathogenic agents for humans E.coliO157, which was obtained in this study, and Salmonella spp. should be controlled by heat-treatment.