Yazar "Akata, Ilgaz" seçeneğine göre listele
Listeleniyor 1 - 11 / 11
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Amanita vidua: A new record for Turkish Amanita Section Phalloideae based on morphological and molecular data(Trakya Univ Balkan Yerlesesi Enstituler Binasi, 2024) Akata, Ilgaz; Kumru, Eda; Sahin, Ergin; Acar, Ismail; Kaya, ErtugrulIn the present study, fungal specimens collected from Ye & scedil;ildere village in U & scedil;ak province, T & uuml;rkiye were evaluated. The specimens underwent detailed examination through both morphological and DNA (nrITS rDNA region and TEF1a gene) sequence -based phylogenetic analysis. Based on the evaluation of micro- and macromorphological characteristics, and phylogenetic analyses, the specimen was identified as A. vidua Gasch, G. Moreno & P. A. Moreau. This identification marks A. vidua as a new record for the Turkish Amanita section Phalloideae. The study described this newly recorded species, including specific details such as its location, habitat observations, geographical coordinates, collection date, and photographs showcasing its macro and micromorphological features. Additionally, it also includes illustrations of some microscopic structures of the species. The study is further enriched with SEM images, offering a detailed view of the species characteristics, and the findings are briefly discussed.Öğe ANTIOXIDANT ANTIMICROBIAL OXIDANT AND ELEMENTS CONTENTS OF XYLARIA POLYMORPHA AND X. HYPDXYLON (XYLARIACEAE)(Parlar Scientific Publications (P S P), 2021) Saridogan, Beste Gizem Ozbey; Islek, Cemil; Baba, Hayri; Akata, Ilgaz; Sevindik, MustafaIn the current study, antioxidant, oxidant, and antimicrobial potentials of Xylaria polymorpha (Pers.) Grey. and X hypoxylon (L.) Grey. were determined. Also, the levels of elements accumulated by these fungi were measured. The samples were extracted with ethanol in soxhlet apparatus. Antioxidant and oxidant potentials were determined using Rel Assay kits. Antimicrobial activities were tested using the agar dilution method. Element contents were determined using atomic absorption spectrophotometer. As a result of the studies, TAS, TOS, and OSI values of X hypoxylon were determined as 3.794 +/- 0.188, 8.609 +/- 0.171, and 0.228 +/- 0.015, respectively. TAS, TOS, and OSI values of X. polymorpha were determined as 5.390 +/- 0.176, 14.738 +/- 0.126, and 0.274 +/- 0.008, respectively. Besides, it was observed that the antimicrobial activities of the extracts of mushrooms were effective at 50-400 mu g/mL concentrations. Element contents were determined to be at normal levels compared to literature data. As a result, it was determined that X. polymorpha and X. hypoxylon have antioxidant and antimicrobial potentials.Öğe BIOLOGICAL ACTIVITIES AND HEAVY METAL CONTENTS OF SOME PHOLIOTA SPECIES(Parlar Scientific Publications (P S P), 2021) Islek, Cemil; Saridogan, Beste Gizem Ozbey; Sevindik, Mustafa; Akata, IlgazIn this study, antioxidant, oxidant, and antimicrobial activities of some Pholiota species (Pholiota adiposa (Batsch) P. Kumm., P lubrica (Pers.) Singer and P. squarrosa (Vahl) P. Kumm.) were determined. Also, the Fe, Cu, Zn, Pb, Cr, Mn, Ni, and Cd levels accumulated in the fruiting bodies of these species were measured. Ethanol extracts of the mushroom specimens have been prepared. The antioxidant and oxidant status of the extracts were determined using Rel Assay kits. Also, the antibacterial and antifungal activities of the extracts were determined using the agar dilution method. Heavy metal levels of the samples were determined using atomic absorption spectrophotometer. As a result of the studies, the highest TAS value was determined in P squarrosa. The highest TOS value was determined in P lubrica. Generally, antibacterial and antifungal activities were observed in P lubrica, Generally, element levels fell within the literature ranges. Besides, it is thought that all three types of species can be used as an indicator due to their high Ni contents. As a result, it was determined that P adiposa, P lubrica and P squarrosa can be used as antioxidant and antimicrobial natural agents.Öğe Biological Activities of Wild Poisonous Mushroom Entoloma sinuatum (Bull.) P. Kumm (Boletales)(Kahramanmaras Sutcu Imam Univ Rektorlugu, 2022) Bal, Celal; Baba, Hayri; Akata, Ilgaz; Sevindik, Mustafa; Selamoglu, Zeliha; Akgul, HasanIn many studies conducted in recent years, it has been found that the beneficial natural ingredients found in mushrooms are very important in curing various diseases and eliminating disease factors. It has been reported that fungi that spread in different countries have biological activities. In this study, antioxidant level (TAS). oxidant level (TOS), and antibacterial and antifungalactivity of Entoloma sinuatum (Bull.) P. Kumm were determined. Ethanol extract of the mushroom was extracted in a Soxhlet apparatus. Antioxidant and oxidant levels were determined using Rel Assay TAS and TOS kits. Also. antimicrobial activity was tested against bacteria l and fungal strains using the agar dilution method. As a result of the studies. the TAS value of the mushroom was determined 2.64 +/- 0.15. the TOS value vas 6.58 +/- 0.23, and the OSI (Oxidative stress index) value was 0.25 +/- 0.02. In addition, it was determined that the ethanol extract of the mushroom was effective against bacteria at 200 and 400 mu g/mL, and against fungi at 50 mu g/mL concentrations. As a result, it was determined that E. sinuatum is a natural antioxidant and antimicrobial agent.Öğe Bjerkandera adusta Collected from Niğde: Analysis of Total Phenolic Compound, Antioxidant, vnd Antimicrobial Properties(2022) Ildız, Elif; Canpolat, Şükrü; İşlek, Cemil; Canpolat, Elif; İşlek, Yasemin; Akata, IlgazMacrofungi have long been used as food and medicinal purposes by humankind. This study aimed to identify a macrofungus sample collected from Niğde, Türkiye in 2019 via molecular techniques and determine the content of total phenolic compounds, antioxidant and antimicrobial activities. According to data obtained from the sequence analysis of ITS fragment of rDNA the macrofungus sample was identified as Bjerkandera adusta (Willd.) P. Karst (1880). The sample was extracted with ethanol and methanol. Total phenolic compound content, antioxidant activity via DPPH scavenging method and antimicrobial activity via disc diffusion method of B. adusta was determined by using these extracts. The amount of total phenolic compounds was found as 772.28 ?g GAE/mL for a methanolic extract of B. adusta. The ethanol extract of B. adusta showed 79.66% scavenging activity of 0.1 mM DPPH solution. The highest inhibition zone diameter was measured as 28±1 mm against P. aeruginosa by ethanolic extract, while the lowest antimicrobial activity was found in 15 ?L methanol extract against S. typhimurium with an inhibition zone diameter of 8.7±1.2 mm.Öğe DETERMINATION OF ANTIOXIDANT, ANTIMICROBIAL, DNA PROTECTIVE ACTIVITY AND HEAVY METALS CONTENT OF LAETIPORUS SULPHUREUS(Parlar Scientific Publications (P S P), 2018) Sevindik, Mustafa; Akgul, Hasan; Dogan, Muhittin; Akata, Ilgaz; Selamoglu, ZelihaSince ancient times, people consumed mushrooms as nutrients, especially during the rainy season. The present study aimed to determine the therapeutic potential of an edible mushroom, Laetiporus sulphureus (Bull.) Murrill. Thus, its antioxidant activity was determined by DPPH method and antimicrobial activity was determined with modified agar dilution method. TAS (total antioxidant status), TOS (total oxidant status) and OSI (oxidative stress index) values were determined using Rel Assay kits. PBR 322 supercoiled DNA was used to identify the DNA protective activity. Heavy metal content was determined with atomic absorption spectrophotometry using the wet decomposition method. In conclusion, it was determined that L. sulphureus demonstrated high biological activity and it was considered that the mushroom can be used as a natural resource in alternative medicine.Öğe Lepiota castanea mushroom growing in Turkiye does not contain phallotoxins and amatoxins(Pergamon-Elsevier Science Ltd, 2024) Yilmaz, Ismail; Akata, Ilgaz; Horoz, Ersan; Kaya, ErtugrulThe number of poisoning cases caused by the Lepiota genus is globally increasing. This genus has more poisonous species than the Amanita genus, and many Lepiota species can cause severe toxicity and death if ingested. As recognized in the literature, L. castanea is a toxic species containing amatoxin. Although crude analytical methods have shown that L. castanea contains amatoxins, more recent and sensitive analyses suggest otherwise. Toxin concentrations can vary even among the same fungal species due to geographical and climatic differences. Therefore, this confusion can be resolved by analyzing L. castanea toxins from different geographical regions. This study aimed to demonstrate the toxin levels of L. castanea collected from forests in different regions of Turkiye (Istanbul and Kocaeli) using sensitive methods. The collected mushrooms were analyzed for alpha amanitin, beta amanitin, gamma amanitin, amanin, phallacidin, and phalloidin levels using RP-HPLC-UV and LCESI-MS/MS methods. L. castanea mushroom was found to be free of amatoxin and phallotoxin. Our study revealed for the first time that L. castanea mushrooms from different geographical regions of Turkiye do not contain amatoxin and phallotoxin. Supporting these findings with new studies from different parts of the world would be appropriate.Öğe Oxidant/antioxidant potentials and heavy metal levels of Pisolithus arhizus and its effects on cardiovascular diseases(National Institute of Science Communication and Information Resources, 2021) Sevindik, Mustafa; Ajaz, Mahvish; Özdemir, Betül; Akata, Ilgaz; Selamoğlu, ZelihaPisolithus arhizus (Scop) Rauschert is a cosmopolitan mushroom in warm temperate regions of the world and forms ectomycorrhizas associations with a wide range of tree species (both angiosperms and gymnosperms). P. arhizus samples were collected from a pine forest in Antalya province (Turkey). Powdered mushroom samples were extracted with ethanol (EtOH) using a Soxhlet apparatus at 50 °C, then concentrated under pressure at 40 °C in a rotary evaporator, and stored at 4 °C in airtight containers. Rel Assay Kits were used to determine the total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) of mushroom extracts. The elemental contents of the mushrooms were then determined using the atomic absorption spectrophotometer. It was determined that the heavy metal content in P. arhizus were found to be generally at optimal levels and the TOS value of mushroom was exceptional. Heavy metals are toxic, they may have chronic degenerative changes on organs. Vascular effects of heavy metals may contribute to a variety of pathologic conditions. Heavy metals resulting in pathophysiological changes causes atherogenic events like increased oxidative stress, inflammatory response, and coagulation activity. It has been determined that P. arhizus may be an important source of antioxidants. © 2021, National Institute of Science Communication and Information Resources. All rights reserved.Öğe Potential Cardiogenic Effects of Poisonous Mushrooms(2021) Sevindik, Mustafa; Özaltun, Betül; Braidy, Nady; Akgül, Hasan; Akata, Ilgaz; Selamoğlu, ZelihaNatural resources have been the savior of people at every stage of human history. Mushrooms are very valuable food sources, especially in the rainy seasons. They can be grouped as poisonous, edible or inedible, depending on their nutritional status. These groups have positive or negative effects. Some types of mushrooms have different medicinal properties thanks to their bioactive compounds. It is necessary to characterize their toxicological profiles, especially before using mushroom species for human consumption, as toxic substances are identified even in some edible species. Mycetism, known as mushroom poisoning, is an international health problem. There are those that cause deadly poisoning and limited symptoms of poisoning in mushrooms. Poisonous mushrooms are divided into many classes as cytotoxic, neurotoxic, myotoxic, gastroirritan mushrooms according to their types or symptoms they cause. It is known that mushroom poisoning can cause serious toxicity on the liver, kidneys and central nervous system. However, its effect on heart function has not been determined yet. This study focused on poisonous mushrooms and their cardiological effects.Öğe The Mosses of Ankara University Beşevler 10. Yıl Campus Area (Ankara-Turkey)(2021) Ezer, Tülay; Akata, Ilgaz; , DenizThe present study is based on moss specimens collected from several urban habitats in Ankara University Beşevler 10. Yıl Campus in the year 2019. As a result of identifications of 94 bryophyte specimens collected from the campus area, a total of 28 species belonging to 7 families and 17 genera were determined. Six of them are new record for B7 grid-square. Pottiaceae (9 species) and Orthotrichaceae (6 species) are the two largest families in the campus area.Öğe Total Phenolics, Antioxidative and Antimicrobial Activities of Some Edible Ascomycota Collected from Niğde(2021) Canpolat, Şükrü; Akata, Ilgaz; İşlek, Yasemin; Canpolat, Elif; İşlek, CemilMushrooms have been used as food and medicinal purposes for centuries. In this study, some edible\rAscomycota were collected in 2018 and 2019 from Niğde, Turkey. Molecular identifications of the\rsamples were performed afterward. PCR products were sent for sequence analysis after ITS\rfragments were amplified with suitable primers concerning DNA isolation of samples. The obtained\rdata were compared with the Genbank database for samples’ determination. The mushroom samples\rwere determined to be Terfezia claveryi Chatin and Morchella esculenta (L.) Pers. Samples were\rextracted by ethanol and methanol to determine antimicrobial activity using the disc diffusion\rmethod. Antimicrobial zones against microorganisms were measured. Then DPPH radical\rscavenging effects and total phenolic content of T. claveryi and M. esculenta mushroom samples\rwere studied. The highest antimicrobial activity was observed in ethanol extracts of M. esculenta.\rWhen the DPPH radical scavenging activities are taken into account, the activity is increased\rdepending on the concentration. The highest DPPH scavenging activity was found in the extract of\rM. esculenta with 40.86%. It was determined that the total phenolic substances in methanol extracts\rof mushroom samples varies between 533.28 ± 1,15-537.34 ±2.20 ?g GAE / mg extract.
