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Öğe Characterization of Potato Golden Cyst Nematode Populations (Globodera rostochiensis) in Turkey(Friends Science Publ, 2020) Toktay, Halil; Evlice, Emre; Imren, Mustafa; Ozer, Goksel; Ali, Muhammad Amjad; Dababat, AbdelfattahGolden potato cyst nematode, Globodera rostochiensis (Wollenweber) Behrens, is one of the most important soilborne pathogens causing economic losses in potato. The nematode is known to occurin several countries including Turkey and has a worldwide regulatory concern. In this study, identification and genetic diversity of G. rostochiensis specimens obtained from the main potato producing areas of Turkey were determined. Twenty -five of 35 soil samples collected from the provinces of Izmir, Nevsehir and Nigde were found to contain G. rostochiensis. The variation between G. rostochiensis populations was determined when examined according to ecological and pathogenic characteristics of nematode in Turkey. The cysts of G. rostochiensis were identified by measuring the morphological characters using perennial patterns, vulval cone, vulval basin and juveniles. Phylogenetic analysis of the Large Sub Unit (LSU) region of rDNA sequences was used to assess the inter or intra phylogenetic relationships between the nematode populations. The phylogenetic analyses demonstrated that the nematode specimens from Turkey cluster with Globodera spp. and signified the presence of single species of G. rostochiensis. As a result, morphological, morphometric and molecular methods were successfully combined for identification and characterization of G. rostochiensis. The frequency of G. rostochiensis in regulatory samples frompotato -producing areas is becoming increasingly important. The morphological characterization has several complications in the detection of this quarantine nematode, using of this combination is beneficial for a reliable and quick diagnostic for these nematodes which is crucial for regulatory services and growers. The results might help to investigate different ecotypes of G. rostochiensis for comprehensive understanding about physiology, ecology , and biology of the genus Globodera for its effective management in Turkey. (C) 2020 Friends Science PublishersÖğe Cotton Production in Pakistan(John Wiley & Sons Ltd, 2020) Ali, Muhammad Amjad; Farooq, Jehanzeb; Batool, Asia; Zahoor, Adil; Azeem, Farrukh; Mahmood, Abid; Jabran, Khawar[Abstract Not Available]Öğe Transgenic technologies for efficient insect pest management in crop plants(Elsevier, 2020) Saeed, Faisal; Hashmi, Muneeb Hassan; Hossain, Md. Jakir; Ali, Muhammad Amjad; Bakhsh, AllahInsect and pests impose significant yield losses in crops despite the use of pesticidal and nonchemical controls. These increasing yield losses have provided impetus for the development of new management strategies against various pests. In this regard, transgenic technologies have revolutionized agriculture remarkably with the development of pest-resistant crops that have been outstanding in terms of crop productivity and highly advantageous to the farming community worldwide. With the passage of time, these technologies have enabled the scientists to modify and manipulate crop plants and to provide new solutions to solve conventional barriers for the improvement of economic traits of crops. The advancements in cell biology to regenerate plants from single cells or organized tissues provide prerequisite for the practical use of genetic engineering in crops improvement. The use of insect-resistant endotoxins from Bacillus thuringiensis in commercialized crops has resulted in increased crop yield since their introduction. The plant chloroplastic genome has also been engineered to encode resistance against insects pests. Several transgenic approaches have been employed to enhance resistance in plants against plant parasitic nematode as well. More recently, new and robust genetic engineering techniques like RNAi and Crispr-Cas9 have also proved their usefulness as a potential strategy in crop improvement for the control of pests. This chapter provides comprehensive insights and discussion of use of modern transgenic technologies in today’s agriculture and integration to efficient integrated pest management. © 2020 Elsevier Inc. All rights reserved.