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    Antifeedant activity and growth inhibition effects of some plant extracts against larvae of Colorado potato beetle [Leptinotarsa decemlineata Say (Col: Chyrsomelidae)] under laboratory conditions
    (ENTOMOLOGICAL SOC TURKEY, EGE UNIV, 2015) Alkan, Mustafa; Gokce, Ayhan; Kara, Kenan
    Antifeedant activity and growth inhibition effects of Achillea millefolium L. (Asteraceae), Heracleum platytaenium Boiss (Apiaceae), and Humulus lupulus L. (Cannabaceae) extracts were tested on the third instar larvae of Colorado potato beetle (Leptinotarsa decemlineata Say, Col: Chyrsomelidae) under laboratory conditions. Plant extracts were obtained treating "cones" of H. lupulus, leaves and stems of H. platytaenium and leaves, stems and flowers of A. millefolium with methanol using maceration technique. The plant extracts (50 g/L) were applied to potato leaflets and consumption area for individual insect was measure with a planimeter after 24, 48 and 72h. The single dose screening bioassay revealed that all tested plant extracts possessed antifeedant activity against the beetle larvae at 50 g/L concentration and average consumed leaf area were 0.04 cm(2), 0.05 cm(2) and 0.30 cm(2) for H. lupulus, H. platytaenium and A. millefolium respectively. In dose response bioassay, it was revealed that there was a negative correlation between the averages consumed leaflet area and the plant extract dose. The greatest antifeedant activity was seen in H. lupulus extract treated leaflets at 10 g/L concentration and the average consumed leaflet area was 0.68 cm(2) after 72 h. The H. platytaenium and H. lupulus extracts had similar effects on the larval growth indices. Both plants extracts had 1.2 growth index values at 30 g/L concentration. These results indicate that the H. platytaenium and H. lupulus extracts could have significant potential as antifeedant and larval growth inhibition agent in the control of larvae of Colorado potato beetle.
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    Tanacetum abrotanifolium (L.) Druce (Asteraceae) ekstraktlarının Sitophilus granarius ile Sitophilus oryzae (Coleoptera: Curculionidae)'ye uzaklaştırıcı etkilerinin zorunluluk testleri ile belirlenmesi
    (2015) Alkan, Mustafa; Gökçe, Ayhan; Çam, Halit
    Bu çalışmada Tanacetum abrotanifolium (L.) Druceun gövde ve çiçek ekstraktlarının iki önemli depo zararlısı Sitophilus granarius (L.) (Buğday biti) ve Sitophilus oryzae (L.) (Pirinç biti) (Coleoptera: Curculionidae)ye karşı uzaklaştırıcı etkileri laboratuvar şartlarında değerlendirilmiştir. Çalışmada bitki ekstraktlarının uzaklaştırıcı etkisi zorunluluk testleri ile belirlenmiştir. Bitkinin gövde ve çiçek ekstraktları, farklı çözücüler olan hekzan, etil asetat ve methanol kullanılarak maserasyon tekniği ile elde edilmiştir. Zorunluluk testlerinde en yüksek uzaklaştırıcı etki Pirinç biti için 48. saat sonunda %58.96 çiçek hekzan ekstraktında saptanmıştır. Buğday biti için ise en yüksek uzaklaştırıcı etki 48. saat sonunda %71.40 ile gövde etil asetat ekstraktında saptanmıştır. Çalışma sonuçları T. abrotanifoliumun S. granarius ve S. oryzae ile mücadelede kullanılma potansiyelinin olduğunu ortaya koymuştur.
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    Toxicological and behavioral effects of some plant extract on Colorado potato beetle, Leptinotarsa decemlineata Say, 1824 (Coleoptera: Chrysomelidae)
    (Entomological Soc Turkey, Ege Univ, 2017) Alkan, Mustafa; Gokce, Ayhan
    Repellent, ovicidal and oviposition deterrent effects of six plant extracts [Heracleum platytaenium Boiss (Apiaceae), Humulus lupulus L. (Cannabaceae), Achillea millefolium L. (Asteraceae), Acanthus dioscoridis L. (Acanthaceae), Phlomoides tuberosa (L.) Moench (Lamiaceae), Bifora radians Bieb. (Apiaceae)] were tested on Leptinotarsa decemlineata Say, 1824 (Coleoptera: Chrysomelidae) under laboratory conditions. Methanol extracts prepared from plant vegetative components were tested on L. decemlineata. Heracleum platytaenium extract was significantly more toxic against the egg stage than all other extracts, except for A. millefolium 5 d after treatment. It was followed by A. millefolium extract reducing the egg hatch rate to 15%. Significant mortality was not observed in the case of other plant extracts. In the second series of experiments, different dose-response bioassays with H. platytaenium against L. decemlineata eggs were conducted. The lowest egg hatch rate of 1% was observed at 7.5% [w/v (plant extract/acetone]. The greatest oviposition deterrent effect was seen with the H. platytaenium extract treatment, which resulted in no egg laying. Plant extracts showed a high level of repellent activity to L. decemlineata and their activity increased with extended incubation time. The greatest repellency was observed with the A. millefolium extract treatment, which gave 0.01% repellency in the first 15 min. These results show that H. platytaenium extract could be a useful toll in the control of L. decemlineata.