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Öğe Effect of high hydrostatic pressure on seed germination, microbial quality, anatomy-morphology and physiological characteristics of garden cress (Lepidium sativum) seedlings(TAYLOR & FRANCIS LTD, 2013) Islek, Cemil; Altuner, Ergin Murat; Ceter, Talip; Alpas, HamiHigh hydrostatic pressure is a non-thermal food processing technology that is found to increase the percentage of germination, decrease the germination time and improve the microbial quality of seeds. In this study, pressures of 100-400MPa for 10min at 30 degrees C are used to compare the percentage of germination, the microbial quality of seeds, chlorophyll a and b, and total phenolic compounds concentrations in seedlings, and the anatomy-morphology characteristics of garden cress. Enhanced reductions of total aerobic mesophilic bacteria, total and fecal coliforms, and yeast and mould populations in seeds were observed, especially at 300MPa. In addition, the percentage of germination, chlorophyll content and phenolic compounds concentrations, fresh and dry weights, and hypocotyl lengths of the seedlings are higher than those of all samples, where the percentage of germination is equal to controls but higher than other samples, and radicula length is lower than controls but higher than others.Öğe Investigation of antifungal activity of ononis spinosa L ASH used for the therapy of skin infections as folk remedies(Ankara Microbiology Society, 2010) Altuner, Ergin Murat; Çeter, Talip; Lşlek, CemilTraditional folk remedies used for centuries come up focus of interest in recent years, due to the trend of use of herb-derived natural products. In addition, increasing morbidity and mortality rates of opportunistic fungal infections and accelerating antifungal resistance rates of fungi lead to the use of alternative therapies with herb-derived preparations as novel antifungals. Ononis spinosa L. (spiny restharrow), which is classified in Leguminosae family, is one of the plants used in herbal medicine as folk remedies for the treatment of skin lesions and/or infections as well as many other disorders. Antibacterial, antifungal, anti-inflammatory and analgesic effects of Ononis spinosa (OS) have already been supported by different studies. The roots and aerial sections of OS are the mainly employed parts for application, however local communities inhabiting at southeastern parts of Anatolia, Turkey, employ the ashes of OS widely to heal the skin infections. There have been no reports about the antifungal activity of OS ashes as far as the current literature is concerned. The aim of this study was to investigate the antifungal activity of ashes of OS, collected from a rural area located at Southeast Anatolia. Ashes of OS have been obtained by burning the plant samples at 400?C, and extracted in sterile distilled water and ethanol. The efficacy of aqueous and ethanol extracts of OS ashes were tested against 10 fungi, of which one was a Candida albicans standard strain (ATCC 95071) and the others were clinical isolates (C.albicans, Candida glabrata, Candida tropicalis, Candida krusei, Candida guilliermondii, Candida parapsilosis, Candida pelliculosa, Trkhosporon asahii, Trichophyton rubrum). Antifungal susceptibility test was performed by disc diffusion (DD) method and the results were confirmed with minimum inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) values determined by microdilution method. The results indicated that both aqueous and ethanol extracts of OS ash showed antifungal activity against C. albicans ATCC 95071 (DD inhibition zones were 16 and 15 mm, respectively; MIC= 1.25 ug/ml, MFC= 1.25 ug/ml), whereas against C.glabrata clinical isolate only ethanol extract exhibited antifungal activity (DD inhibition zone= 10 mm, MIC= 5.00 ug/ml, MFC= 40.00 ug/ml). No antifungal effect was detected against the other clinical Candida spp, T.asahii and T.rubrum isolates. In conclusion, since our results emphasize that extracts of OS ash that traditionally used for skin disorders, showed promising degrees of antifungal activity against some of Candida strains, these preliminary data should be supported by further large-scale studies.Öğe INVESTIGATION OF ANTIFUNGAL ACTIVITY OF ONONIS SPINOSA L. ASH USED FOR THE THERAPY OF SKIN INFECTIONS AS FOLK REMEDIES(ANKARA MICROBIOLOGY SOC, 2010) Altuner, Ergin Murat; Ceter, Talip; Islek, CemilTraditional folk remedies used for centuries come up focus of interest in recent years, due to the trend of use of herb-derived natural products. In addition, increasing morbidity and mortality rates of opportunistic fungal infections and accelerating antifungal resistance rates of fungi lead to the use of alternative therapies with herb-derived preparations as novel antifungals. Ononis spinosa L. (spiny restharrow), which is classified in Leguminosae family, is one of the plants used in herbal medicine as folk remedies for the treatment of skin lesions and/or infections as well as many other disorders. Antibacterial, antifungal, anti-inflammatory and analgesic effects of Ononis spinosa (OS) have already been supported by different studies. The roots and aerial sections of OS are the mainly employed parts for application, however local communities inhabiting at southeastern parts of Anatolia, Turkey, employ the ashes of OS widely to heal the skin infections. There have been no reports about the antifungal activity of OS ashes as far as the current literature is concerned. The aim of this study was to investigate the antifungal activity of ashes of OS, collected from a rural area located at Southeast Anatolia. Ashes of OS have been obtained by burning the plant samples at 400 degrees C, and extracted in sterile distilled water and ethanol. The efficacy of aqueous and ethanol extracts of OS ashes were tested against 10 fungi, of which one was a Candida albicans standard strain (ATCC 95071) and the others were clinical isolates (C.albicans, Candida glabrata, Candida tropicalis, Candid krusei, Candida guilliermondii, Candida parapsilosis, Candida Trichosporon asahii, Trichophyton rubrum). Antifungal susceptibility test was performed by disc diffusion (DD) method and the results were confirmed with minimum inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) values determined by microdilution method. The results indicated that both aqueous and ethanol extracts of OS ash showed antifungal activity against C. albicans ATCC 95071 (DD inhibition zones were 16 and 15 mm, respectively; MIC= 1.25 mu g/ml, MFC= 1.25 mu g/ml), whereas against C.glabrata clinical isolate only ethanol extract exhibited antifungal activity (DD inhibition zone= 10 mm, MIC= 5.00 mu g/ml, MFC= 40.00 mu g/ml). No antifungal effect was detected against the other clinical Candida spp, T.asahii and T.rubrum isolates. In conclusion, since our results emphasize that extracts of OS ash that traditionally used for skin disorders, showed promising degrees of antifungal activity against some of Candida strains, these preliminary data should be supported by further large-scale studies.Öğe The effect of high hydrostatic pressure on the physiological and biochemical properties of pepper (Capsicum annuum L.) seedlings(TAYLOR & FRANCIS LTD, 2015) Islek, Cemil; Altuner, Ergin Murat; Alpas, HamiHigh hydrostatic pressure is a non-thermal food processing technology, which also has several successful applications in different areas besides food processing. In this study, Capsicum annuum L. (pepper) seeds are subjected to 50, 100, 200 and 300MPa pressure for 5min at 25 degrees C and the seedlings of HHP processed seeds are used to compare percentage of seed germination and biochemical properties such as chlorophyll a, b and a/b, proline content, total protein, carotenoid, malondialdehyde, glucose, fructose and phenolic compounds concentrations. As a result of the study, it was observed that there are remarkable changes in terms of biochemical properties especially for seedlings, whose seeds were pressurized at 200 and 300MPa. More detailed studies are needed to put forward the mechanism behind the changes in biochemical properties.Öğe Triakontanol ve yüksek hidrostatik basıncın serbest ve tutuklanmış capsicum annuum L. hücre süspansiyon kültüründe kapsaisin üretimi üzerine etkisi(2015) İşlek, Cemil; Alpas, Hami; Koç, Esra; Altuner, Ergin Murat[Abstract Not Available]Öğe Using high hydrostatic pressure as an abiotic elicitor strategy for improving capsaicin production in free and immobilized cell suspension cultures of Capsicum annuum L.(Taylor & Francis Ltd, 2021) Islek, Cemil; Koc, Esra; Altuner, Ergin Murat; Alpas, HamiIn this study, the effects of immobilization and high hydrostatic pressure (HHP) on capsaicin production in cell suspension culture of pepper seeds at different application times were investigated. Callus cultures were obtained from in vitro germinated hypocotyl explants of pepper seedlings and cell suspensions were prepared from calluses. Immobilized cell suspension cultures with calcium alginate and free cell suspension cultures were obtained by using these cell suspensions. 50, 100, 200, 300 and 400 MPa were applied to both free and immobilized cell suspensions as an elicitor. When all the results were compared, the highest amount of capsaicin was achieved in immobilized cell suspensions on the tenth day as 293.187 mu g/g f.w at 400 MPa pressure. As a result, it can be concluded that the combined application of immobilization and elicitor (HHP) caused significant increases (p < .05) in the amount of capsaicin.
