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Öğe Characterization of stress induced by copper and zinc on cucumber (Cucumis sativus L.) seedlings by means of molecular and population parameters(ELSEVIER SCIENCE BV, 2012) Aydin, Semra Soydam; Gokce, Esra; Buyuk, Ilker; Aras, SumerContamination of plants with heavy metals could result in damage in DNA, such as mutations and crosslinks with proteins. These altered DNA profiles may become visible in changes such as the appearance of a new band, or loss of an existing band, in the random amplified polymorphic DNA (RAPD) assay. In this study, various concentrations of copper and zinc salts were applied to cucumber seedlings during germination. Results displayed abnormalities in germination and also changes in root elongation, dry weight and total soluble protein level. All treatment concentrations (40, 80, 160, 240, 320, and 640 mg/L) used in the study caused a decrease/delay in germination of the cucumbers to different extents. Inhibition or activation of root elongation was considered to be the first effect of metal toxicity in the tested plants. Application of the metal salts and the combined solutions on cucumber (Cucumis sativus L) seedlings revealed similar consequences for total soluble protein level, dry weight and ultimately in inhibitory rates as well. The data obtained from RAPD band-profiles and genomic template stability (GTS) showed results that were consistent with the population parameters. In this regard, we conclude that molecular marker assays can be applied in combination with population parameters to measure genotoxic effects of heavy metals on plants. (C) 2012 Elsevier B.V. All rights reserved.Öğe Defence responses in tomato (Lycopersicum esculentum L.) contaminated with different concentration of Cu2+(ELSEVIER SCIENCE BV, 2012) Buyuk, Ilker; Aydin, Semra Soydam; Bolukbasi, Ekrem; Aras, Sumer[Abstract Not Available]Öğe Expression analysis of catalase and superoxide dismutase genes in Lycopersicum esculentum L. under zinc stress(ELSEVIER SCIENCE BV, 2012) Buyuk, Ilker; Aydin, Semra Soydam; Altunkaynak, Ersin; Aras, Sumer[Abstract Not Available]Öğe Genotoxic effect of cadmium in okra seedlings: Comperative investigation with population parameters and molecular markers(TRIVENI ENTERPRISES, 2013) Aydin, Semra Soydam; Basaran, Esin; Cansaran-Duman, Demet; Aras, SumerPlants are considered as good bioindicators because of their significant role in food chain transfer. They are also easy to grow, adaptable to environmental stresses and can be used for assaying a range of environmental conditions in different habitats. Thus, many plant species have been used as bioindicators. In order to evaluate the genotoxic effect of cadmium, okra (Abelmoschus esculantus L.) seedlings were treated with different concentrations (30, 60, 120 mg l(-1)) of cadmium and investigated for their population parameters such as inhibition of root growth; total soluble protein content, dry weight and also the impact of metal on the genetic material by RAPD analysis. Root growth and total soluble protein content in okra seedlings were reduced with increased Cd concentrations. RAPD analysis indicated formation of new bands mostly at 60 and 120 mg l(-1) Cd treatments. Altered DNA band patterns and population parameters after Cd treatments suggest that okra could be used as an indicator to reveal the effects of genotoxic agents.Öğe Relationships among lipid peroxidation, enzyme activity and gene expression profiles of superoxide dismutase in Lycopersicum esculentum L. exposed to cold stress(ELSEVIER SCIENCE BV, 2012) Aydin, Semra Soydam; Gokce, Esra; Aras, Sumer[Abstract Not Available]
