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Öğe Effect of thermotherapy in combination with merİstem culture for elİmİnatİng potato virus y (Pvy) and potato virus s (pvs) from infected seed stocks(Pakistan Agricultural Scientists Forum, 2019) Ateş S.Y.; İnan S.; Ayyaz M.; Dündar İ.; Yabangülü D.The present study was conducted to investigate the impact of thermotherapy along with meristem culture for eliminating Potato virus Y (PVY) and Potato virus S (PVS). The plantlets were subjected to enzyme linked immunosorbent assay (ELISA) to check the presence of targeted viruses. A total of 110 plants including 65 infected with PVY and 45 with PVS belonging to six potato cultivars (Agria, Russet Burbank, Shepody, Granola, Marabel, and Sante) were subjected to thermotherapy (under constant temperature of 36°C and constant light conditions) for a period of five weeks. The obtained results revealed no PVY and PVS positive plants after five week of thermotherapy. However, plant mortality was observed in cv. Granola, R. Burbank, Shepody and Agria. Cv. Granola was sensitive, whereas, Sante and Marabel were found to be resistant cultivars. Marabel and Sante responded effectively to thermotherapy treatment by producing PVY and PVS negative plants with no plant mortality after application of thermotherapy. The study showed that propagation of clean production material is possible by applying thermotherapy treatment along with tissue culture method by using meristem culture and shoot tips cultures. © 2019, Pakistan Agricultural Scientists Forum. All rights reserved.Öğe First report of Grapevine roditis leaf discoloration-associated virus infecting grapevine (Vitis vinifera) in Turkey(American Phytopathological Society, 2018) Ulubaş Serçe Ç.; Altan B.; Bolat V.; Ayyaz M.; Çifçi O.; Önder S.; Maliogka V.I.[No abstract available]Öğe Screening of different groundnut, aarachis hypogaea L. (Fabaceae: Fabales) cultivars against red hairy caterpillar, amsacta albistriga W. (Arctiidae: Lepidoptera) population in relation to leaf infestation, physico-morphic characters, abiotic factors and yield(University of Punjab (new Campus), 2015) Baig F.; Ayyaz M.; Javed H.Field screening of six groundnut cultivars (BARD-92, BARD-699, BARD-479, BARI-2000, Golden and Chakori) in randomized complete block design with three replications was conducted against red hairy caterpillar (RHC), Amsacta albistriga (Walker), population in relation to leaf infestation, physico-morphic characters (leaf area and plant height), abiotic factors (temperature, rainfall and humidity) and their ultimate impact on yield. Highest RHC population and leaf infestation was observed on BARD-699 and the lowest on BARD-479 throughout the season. Irrespective of plant height, leaf area seemed to effect insect infestation with minimum infestation on BARD-479 with maximum leaf area while minimum was observed on BARD-699 (maximum insect infestation). The plant height of BARD-699 was found to be maximum, whereas minimum plant height was observed on BARD-479. Maximum pod yield was recorded on Bard-479 and minimum of Bard-699. The order of cultivar resistance against RHC population during the whole crop duration was BARD-479 < BARI-2000 < Golden < BARI-92 < Chakori < BARD-699. RHC population showed significant and positive correlation with leaf infestation, and non-significant and negative correlation with leaf area. Non-significant and positive correlation was found among RHC population and plant height, and significant but negative relationship was noted between mean temperature and RHC population. Although effect of rainfall was significant and positive on RHC population, there was significant and positive correlation between relative humidity and population of RHC. A significant and negative correlation between RHC population and yield was recorded. Based on the results, high yielding cultivar Bard-479 was found resistant and low yielding Bard-699 was found susceptible against RHC. Copyright 2015 Zoological Society of Pakistan.