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    Determination of resistance against to Cereal cyst nematode, Heterodera avenae (Wollenweber, 1924) in some wheat germplasm
    (ENTOMOLOGICAL SOC TURKEY, EGE UNIV, 2013) Imren, Mustafa; Toktay, Halil; Bozbuga, Refik; Dababat, Amer; Ozkan, Hakan; Elekcioglu, I. Halil
    The cereal cyst nematode, Heterodera avenae is one of the most important pests of wheat in many countries in the world with different climatic types. Heterodera. avenae is commonly distributed in the Eastern Mediterranean region of Turkey and all populations investigated belong to the Hal group, Ha21 pathotype. This study was aimed to found resistance sources from the national wheat varieties, wheat wild genotypes and international wheat genotypes against H. avenae Karlik Adana population (Ha21 pathotype). Results showed that four national wheat varieties, seventeen wheat wild genotypes and twenty three international wheat genotypes were found to be moderately resistant against to Eastern Mediterranean Region of Turkey H. avenae population. Among these genotypes, the national bread wheat variety, Adana 99 (PFAU/SERI82//BOG"S"), some wild genotypes and international genotypes can be used in national wheat breeding programmes. However, Crel was not showed completely resistant against H. avenae. Additionally resistance sources in of Turkish national wheat and wild genotypes needs to be determined.
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    Effect of Mi Gene and Nematode Resistance on Tomato Genotypes Using Molecular and Screening Assay
    (Allerton Press Inc, 2020) Bozbuga, Refik; Dasgan, H. Yildiz; Akhoundnejad, Yelderem; Imren, Mustafa; Gunay, Ozlem C.; Toktay, Halil
    The Mi gene is known to involve in the resistance response to some Root knot nematodes, Meloidogyne spp. in tomato plants Lycopersicon esculentum. Root knot nematodes cause significant damages to almost all crops in the world. Among Meloidogyne species, Meloidogyne incognita is one of the most damages species causing a vast amount of crop loss including tomato plants. The resistance gene may be found in some tomato genotypes or cultivars. Determining the resistance gene, Mi, in tomato cultivars is very important to control root knot nematodes. For this purpose, this study was conducted in 99 tomato genotypes that were screened for resistance against Meloidogyne incognita in molecular and screening assays. Results revealed that Mi gene was only determined in one genotype (Tom113). The result of the screening assay indicated that one of the genotypes (Tom113) showed an immune reaction against the nematode; two genotypes (Tom146, Tom141) were found intermediate; 17 genotypes moderately susceptible, 43 genotypes susceptible and 36 genotypes found highly susceptible. Results of the screening assay confirmed the presence of Mi gene in a tomato genotype, which accompanied with a decreased ability for the nematode in causing the infection. Immune and some promising genotypes are thought as important genetic tools for breeding studies for future works.
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    Heterodera schachtii (Nematoda: Heteroderidae) Associated with Cabbage-Cultivation Systems in Anatolia Region of Türkiye
    (Mdpi, 2024) Akyol, Gulsum Badel; Yuksel, Ebubekir; Elci, Eminur; Bozbuga, Refik; Dababat, Abdelfattah; Imren, Mustafa; Toktay, Halil
    Heterodera schachtii (Schmidt, 1871) (Nematoda: Heteroderidae) is one of the most widespread plant-parasitic nematodes (PPNs) associated with cabbages, which cause severe yield losses in cruciferous vegetables. This study aimed to improve the current understanding of the prevalence and detection of H. schachtii in the cabbage-growing areas of Ni & gbreve;de Province, T & uuml;rkiye. Field surveys were conducted between November and December 2021, and 100 soil samples were collected immediately after cabbage harvesting. Heterodera schachtii populations were identified by morphological and morphometric methods along with Internal Transcribed Spacer of the ribosomal region (ITS-rDNA) and Cytochrome Oxidase Subunit (COI-mtDNA) sequencing. The mean body length of H. schachtii was 463 +/- 7 mu m, while stylet and hyaline length ranged between 20.7-27.8 mu m and 20.1-32.1 mu m, respectively. Nearly half of the surveyed areas were infested with H. schachtii with a 41% incidence rate. However, the Merkez District had the highest proportion of infested fields with an over 51% incidence rate. The population density was determined in 41 samples with a mean of 79.5 cysts per 250 g of soil. These results will help to determine the control and management strategies of H. schachtii.