Yazar "Caglayan, K." seçeneğine göre listele

Listeleniyor 1 - 6 / 6
  • Küçük Resim Yok
    Öğe
    Comparison of Serological and Molecular Detection Methods for Testing Individual and Composite Samples Using PPV-M and PPV-T Isolates
    (INT SOC HORTICULTURAL SCIENCE, 2015) Gazel, M.; Serce, C. Ulubas; Caglayan, K.; Safarova, D
    Sharka disease of stone fruit trees caused by Plum pox virus (PPV) was first described in 1968 in a limited area of Turkey, but during the last decade the disease has progressively spread to a large part of the country. Although PPV-Rec and -D strains were found in Turkey, the most common PPV strains were detected as PPV-M and PPV-T. In this study, DAS-ELISA (5B-IVIA/AMR) monoclonal antibody) and Spot Real-time RT-PCR techniques have been evaluated in order to determine the best sampling time and ratio of PPV infected samples in non-infected-infected plant mixtures for detection of PPV-T and PPV-M strains. Dormant buds in winter and fresh leaves in spring from PPV-infected trees were used for testing in 2012. Six repetitions were performed by single (3 leaves or buds from infected plant) or composite plant mixture samples (3 leaves or buds from infected plant + 3 leaves from healthy plant, and the other composite samples, i.e., 3+6 to 3+27). All combinations and all repetitions of composite leaf samples of both strains were detected as positive in Spot Real-time RT-PCR. However, in DAS-ELISA, the number of PPV positive samples decreased for T and M strain in 6th composite (3 infected+12 healthy leaves) and in 9th composite (3 infected+21 healthy leaves) in spring, respectively. At least 3 repetitions in all combinations of composite samples for PPV-T and -M were found positive in dormant season by Spot Real-time RTPCR whereas it was negative only in the last composite sample (3 infected+27 healthy buds) of PPV-T by DAS-ELISA.
  • Küçük Resim Yok
    Öğe
    Experimental transmission efficiency of Plum pox virus T by Myzus persicae Sulzer (Homoptera: Aphididae)
    (INT SOC HORTICULTURAL SCIENCE, 2017) Ates, S. Yalcin; Gazel, M.; Serce, C. Ulubas; Caglayan, K.; Caglayan, K
    [Abstract Not Available]
  • Küçük Resim Yok
    Öğe
    First Report of Grapevine Pinot gris virus in Grapevine in Turkey
    (AMER PHYTOPATHOLOGICAL SOC, 2016) Gazel, M.; Caglayan, K.; Elci, E.; Ozturk, L.
    [Abstract Not Available]
  • Küçük Resim Yok
    Öğe
    Fourthy-Five Years of Sharka Disease in Turkey
    (INT SOC HORTICULTURAL SCIENCE, 2015) Caglayan, K.; Serce, C. Ulubas; Gazel, M.; Safarova, D
    Sharka disease in Turkey has firstly been reported in 1968 in Edirne (Marmara region) which is located next to the Bulgarian border. Nowadays, new PPV outbreaks have been reported in Central Anatolia (Ankara, Kayseri), Aegean (Izmir) and Mediterranean regions (Adana, Mersin, Hatay). The distribution of PPV strains was mainly related to the geographical location and the period of PPV introduction in these regions. PPV-M was mainly detected in peach, nectarine and apricot which were recently imported from abroad to the Mediterranean region. PPV-T was detected in apricot and plums in Central Anatolia and in the Aegean Regions where PPV has been endemic and existing for years. These distributions might indicate that new outbreaks may be mainly due to latently infected material that has passed through the border control. Epidemiology and rootstock susceptibility to PPV has also been recently accomplished. A breeding program has been started in 2006 and its main aim is to obtain dried apricot cultivars resistant to PPV and well adapted to Turkish conditions.
  • Küçük Resim Yok
    Öğe
    Investigation of resistance of apricot progeny to Plum pox virus through molecular markers
    (INT SOC HORTICULTURAL SCIENCE, 2017) Ates, S. Yalcin; Gazel, M.; Serce, C. Ulubas; Asma, B. M.; Caglayan, K.; Caglayan, K
    Plum pox virus (PPV) is the causal agent of sharka disease, which is mainly destructive on apricot (Prunus armeniaca L.), plum (Prunus domestica L.) and peach (Prunus persica L.). There are no efficient control methods except using PPV-free propagating materials and planting PPV-resistant or at least less-susceptible rootstocks. Therefore, lots of studies have been conducted in recent years on breeding of PPV-resistant plants. The objective of this study was the introduction and development of marker-assisted selection (MAS) for PPV resistance in F-1 and F-2 progenies of some Turkish apricot cultivars. Local apricot cultivars 'Adilcevaz 5', 'cologlu', 'Sekerpare' and 'cataloglul crossed with PPV-resistant 'Stark Early Orange' (SEO) were screened with molecular markers PGS1.21 and PGS2.23 co segregating with resistance to PPV in 2011. Of all combinations, seven of 20 progeny obtained from SEO x 12 of 34 progeny obtained from SEO x 'Adilcevaz 5, five of 10 progeny obtained from SEO x 'coloklu, 15 of 37 progeny obtained from SEO x `Sekerpare' and eight of 33 progeny obtained from SEO x 'cataloglu' exhibited resistant alleles.
  • Küçük Resim Yok
    Öğe
    Screening for Resistance to Plum Pox Virus in Some Local Turkish Apricot Cultivars and Their Crosses by Molecular Markers
    (INT SOC HORTICULTURAL SCIENCE, 2015) Serce, C. Ulubas; Gazel, M.; Caglayan, K.; Asma, B. M.; Badenes, M. L.; Safarova, D
    Turkey is the most important producer and exporter country of apricot, Prunus armeniaca. Production of apricots for fresh market relies on foreign cultivars grown on Mediterranean and Aegean regions while Malatya is the most important region for production of dry apricots based on local cultivars. Plum pox virus (PPV) in Turkey has been known since 1968, but it was not widespread until recent years. Malatya region has been free of sharka disease so far, but the disease has already been reported from many different provinces since 2006. Because of that, introgression of resistance to PPV in the local cultivars with good pomological characteristics became an important objective for the apricot crop. In the current breeding program, obtaining new cultivars resistant to PPV, selection of resistant seedlings by using molecular markers linked to PPV resistance was aimed at. Nineteen local apricot genitors and progenies obtained from the crosses between the PPV resistant cultivar 'Stark Early Orange' (SEO), 'Harcot' and local cultivars such as 'Hacihaliloglu', 'Kabaasi', 'Hasanbey', 'Cologlu', 'Adilcevaz5', 'Sekerpare', 'MahmudunErigi', 'Soganci' and 'Cataloglu' were screened with markers. The markers PGS1.21 and PGS2.23 co-segregating with resistance to PPV were used to screen a total of 189 apricot progenies. None of the local genitors had alleles linked to PPV resistance. Among the progenies screened, 15 seedlings from 'Sekerpare' by SEO, 12 from 'Adilcevaz5' by SEO, 7 from 'Hacihaliloglu' by SEO, 9 from 'Kabaasi' by SEO, 5 from 'Cologlu' by SEO, 9 from 'Cataloglu' by SEO, 4 from 'Hasanbey' by SEO, and 1 from 'MahmudunErigi' by SEO and none of the 'Harcot' by 'Soganci' presented resistant alleles and were selected for further studies.