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    Seroprevalence of hypodermosis in cattle in Nigde Province of Turkey by comparison of commercial and indirect-ELISA method
    (2013) Karatepe M.; Simsek S.; Karatepe B.; Cayvaz M.; Sevgili M.; Balkaya I.
    The aim of this study was to determine the seroprevalence of hypodermosis in cattle of Nigde province, located in the centre of Turkey. A total of 183 serum samples were collected between December 2009 and February 2010 for this goal. The serum samples were analyzed with a commercial ELISA kit (Pourquier ELISA Hypodermosis bovine serum screening) and an indirect-ELISA (using antigen Hypodermin C) methods. The results of the tests with the commercial ELISA kit showed that 53 (28.9%) out of the 183 cattle were seropositive for hypodermosis. Eleven (18.3%) out of the 60 female and 42 (34.1%) out of the 123 male, were found to be seropositive. The seropositivity rate was calculated as 21.8% (40/183) by indirect- ELISA, and the seroprevalence was 18.3% (11/60) and 23.5% (29/123) in female and males, respectively. A statistically significant correlation was observed when the two test methods for detecting seropositivity of Hypoderma were compared (p<0.001). This study is to the best of our knowledge the first serological survey on hypodermosis in cattle from the Nigde province of Turkey.
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    Seroprevalence of neospora caninum in goats in nigde province [Nigde yöresi keçilerinde neospora caninum'un seroprevalansi{dotless}]
    (2011) Cayvaz M.; Karatepe M.
    This study was performed to investigate seroprevalence of Neospora caninum in goats in Nigde province between February 2010-May 2010. A total of 181 goats which were one year old or more than one year old, were selected from eight diferent study sites (Bahçeli, Centrum-Tepeköy, Bor, Çamardi{dotless} Celaller Village, Ki{dotless}zi{dotless}lca, Çukurkuyu, Uluki{dotless}şla, ve Central Çamardi{dotless}) as the study sample and N. caninum antibodies were tested in these goats serum using ELISA. The 47 out of 181 sera (25.9%) were found to be seropositive for N. caninum. In the serologic examinations, the seropositivity rate was highest in 5 years old (42.3%) while it was lowest in 3 years old (13.8%) in goats. The 6 (37.5%) out of 16 aborted goats and 41 (24.8%) out of 165 non-aborted were detected as seropositive. The highest seropositivity rate of study sites was determined in Çamardi{dotless} as 54.5% in addition to this, none of examined goats in Bahçeli and Ki{dotless}zi{dotless}lca detected seropositivity. The diference between the seropositivity rates to N.caninum among age groups and study sites was statistically significant (P<0.05, P<0.01, respectively) while the diference to N. caninum among aborted and non-aborted goats was statistically insignificant (P>0.05). As a result, this is the first serologic survey for N. caninum performed on goats in Nigde province and neosporosis should be taken into consideration in abortion cases.