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Editing of banana, apple, and grapevine genomes using the CRISPR-Cas9 system
(Elsevier, 2024) Çelik, Selime; Deniz, Utku; Gencer, Orkun; Aksoy, Emre
Among horticultural crops, fruits have an essential place since they are key sources of nutrients such as essential vitamins and minerals, and fruit production is crucial for the economy since it has a large market globally. Apple, banana, and grapevine are highly demanded fruit crops for consumers and producers; therefore, it is no surprise that these fruits are also subjects of molecular studies. Following the publication of the apple, banana, and grapevine genomes, molecular studies in these fruits became more feasible via the utilization of next-generation sequencing (NGS), high-quality de novo assembly, transgenics, and finally, genome editing by CRISPR-Cas9. Currently, the application of advanced genome-editing techniques such as CRISPR-Cas systems pave the way for basic and applied research on apple, banana, and grapevine in a rapid and precise way. With the improvement of CRISPR technologies such as base editing, prime editing, and different types of Cas proteins, the development of new superior cultivars with desirable traits will be possible in the future. © 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.