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    Immobilization of laccase onto a porous nanocomposite: application for textiledye degradation
    (2016) Sedef İlk; Deniz Demircan; Semran Sağlam; Necdet Sağlam; Zakir M. O. Rzayev
    Poly(MA-alt-MVE)-g-PLA/ODA-MMT nanocomposite was prepared by self-catalytic interlamellar graftcopolymerization of L-lactic acid (LA) onto poly(maleic anhydride-alt-methyl vinyl ether) copolymer in the presence ofoctadecyl amine-montmorillonite (ODA-MMT) organoclay. FTIR,1H (13C) NMR, XRD, and SEM-TEM were utilizedfor characterizing the resultant nanocomposite. Laccase fromTrametes versicolorwas immobilized onto the preparednanocomposite by adsorption or covalent coupling. Decolorization of Reactive Red 3 from aqueous solution by laccaseimmobilized on the nanocomposite was studied in different conditions (pH, temperature, dye concentration, and reactiontime) to investigate the decolorization activity with repeated use and storage. The results indicated that more than 77%of the activity of laccase immobilized systems was retained at the end of 10 cycles. The nal decolorization capacity ofthe immobilized laccase was signi cantly higher (65%) than that of free laccase (33%) in the chosen optimized conditions(pH 5, 20◦C, 0.05 mg/mL laccase concentration, and 90 min).