Yazar "Diler, Songul Budak" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim Yok
    Öğe
    Cytogenetic Biomonitoring of Carpet Fabric Workers Using Micronucleus Frequency, Nuclear Changes, and the Calculation of Risk Assessment by Repair Index in Exfoliated Mucosa Cells
    (MARY ANN LIEBERT, INC, 2011) Diler, Songul Budak; Celik, Ayla
    The micronucleus (MN) assay in exfoliated buccal cells is a minimally invasive method for monitoring genetic damage in human populations and is used as an indicator of genotoxic exposition, as it is associated with chromosome aberrations. In this study, we evaluated MN frequencies and other nuclear changes (NCs), such as karyorrhexis (KR), karyolysis (KL), broken egg (BE), and binucleus in buccal mucosa cells of 50 carpet fabric workers (25 smokers and 25 nonsmokers) and 50 healthy control subjects (25 smokers and 25 nonsmokers). Microscopic observation of 2000 cells per individual was performed in both workers and control subjects. In both the control group and the exposed group, for each person a repair index (RI) was calculated via the following formula: (KR+KL)/(BE+MN). The results showed a statistically significant increase in the frequency of MN in buccal epithelial cells of exposed group compared with control group. There is a significant difference between worker and control groups (p<0.001) for RI. We believe that the calculation of RI values, in addition to nuclear changes, presents a new approach in risk assessment in relation to occupational exposure.
  • Küçük Resim Yok
    Öğe
    The MNS16A VNTR polymorphism of the TERT gene in bladder cancer
    (Aves, 2020) Diler, Songul Budak; Polat, Fikriye; Bingol, Gunsel
    Objective: Bladder cancer (BC) is a complex disease that has a high morbidity rate. The MNS16A polymorphism in the TERT gene has been indicated to play a role in the presence of various cancer types and multiple tumor populations. In the present study, our goal was to investigate whether the MNS16A (VNTRs) in the TERT gene was associated with bladder cancer. Material and methods: A total of 70 patients with BC and 120 normal controls were included in the study. The MNS16A (VNTRs) in the TERT gene was amplified using polymerase chain reaction (PCR). The PCR products were visualized on 3% high resolution agarose gel and under a UV light. Results: The MNS16A VNTR-302 allele was found to be the most common allele in both, the patient group (64%) and the control group (62%). The second most common allele was the VNTR-243 allele that occurred at a frequency of around 34% in BC patients and 33% in the controls. VNTR-333 (patient group, 1%; control group, 3%) and VNTR-274 (patient group, 2%; control group, 1%) alleles were reported as the least common alleles in this study. Conclusion: When comparing the frequencies of genetic variants between cases and controls, we observed that our findings did not support the hypothesis that the MNS16A VNTR polymorphism of the TERT gene might regulate cancer susceptibility.
  • Küçük Resim Yok
    Öğe
    The P268S and M863V Polymorphisms of the NOD2/CARD15 Gene in Crohn's Disease and Ulcerative Colitis
    (Allerton Press Inc, 2019) Diler, Songul Budak; Polat, Fikriye; Yaras, Serkan
    We aimed to determine P268S and M863V polymorphisms in NOD2/CARD15 genes associated with an increased risk of developing Crohn's disease (CD) and ulcerative colitis (UC) and to establish correlations between P268S and M863V genotypes in Turkish population. This study included 152 healthy controls and 138 patients (62 CD and 76 UC) with IBD. Our results, the P268S CC genotype was prevalent on patients and controls (45 vs. 66%), followed by genotypes CT (48 vs. 28%) and TT (7 vs. 6%) in CD. The prevalence of genotypes of CC (wild-type), CT (heterozygous mutant) and TT (homozygous mutant) profiles for the P268S polymorphism were 68, 25 and 7% respectively in UC patients, and 66, 28 and 6% respectively in healthy control groups. We were found in wild-type M863V in all subjects and no other mutant band. P268S polymorphism may be associated with CD susceptibility in Turkish population, whereas there is not associated between M863V polymorphisms and patients with IBD in Turkish population.