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    DETERMINATION OF ANTIOXIDANT, ANTIMICROBIAL, DNA PROTECTIVE ACTIVITY AND HEAVY METALS CONTENT OF LAETIPORUS SULPHUREUS
    (Parlar Scientific Publications (P S P), 2018) Sevindik, Mustafa; Akgul, Hasan; Dogan, Muhittin; Akata, Ilgaz; Selamoglu, Zeliha
    Since ancient times, people consumed mushrooms as nutrients, especially during the rainy season. The present study aimed to determine the therapeutic potential of an edible mushroom, Laetiporus sulphureus (Bull.) Murrill. Thus, its antioxidant activity was determined by DPPH method and antimicrobial activity was determined with modified agar dilution method. TAS (total antioxidant status), TOS (total oxidant status) and OSI (oxidative stress index) values were determined using Rel Assay kits. PBR 322 supercoiled DNA was used to identify the DNA protective activity. Heavy metal content was determined with atomic absorption spectrophotometry using the wet decomposition method. In conclusion, it was determined that L. sulphureus demonstrated high biological activity and it was considered that the mushroom can be used as a natural resource in alternative medicine.
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    Phenolic Content and Antioxidant Potential of Terfezia boudieri
    (Gazi Univ, 2018) Sevindik, Mustafa; Pehlivan, Mustafa; Dogan, Muhittin; Selamoglu, Zeliha
    The present study aimed to determine the phenolic content, total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) of Terfezia boudieri Chatin, known as truffle mushroom. In this context, phenolic content was determined with an HPLC device. TAS, TOS and OSI were determined with Rel Assay kits. As a result of the conducted analyses, 30.89 mg/kg gallic acid, 554.64 mg/kg syringic acid and 5.52 mg/kg 4-hydroxybenzoic acid were determined in the mushroom. It was determined that TAS value was 2.332 +/- 0.034, TOS value was 26.945 +/- 0.144 and OSI value was 1.156 +/- 0.011. It was suggested to avoid excessive consumption of mushrooms collected in this region due to the high TOS value. It was also considered that samples collected in appropriate regions could be consumed as a good antioxidant source based on the determined OSI.