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    Identification of potential microRNA markers related to Crimean-Congo hemorrhagic fever disease
    (Wiley, 2019) Arslan, Serdal; Engin, Aynur; Aydemir, Eylem Itir; Sahin, Nil Ozbilum; Bayyurt, Burcu; Sari, Ismail; Cosgun, Yasemin
    Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease caused by the arbovirus Crimean-Congo hemorrhagic fever virus (CCHFV). The CCHFV has a single-stranded RNA genome of negative sense. MicroRNAs (miRNAs) are key players in virus-host interactions and viral pathogenesis. We investigated the miRNA gene expression profiles in patients with CCHF using microarray for the first time in the world. Microarray analysis was performed using mirBase Ver 21 (Agilent Technologies, Santa Clara, CA). All statistical analyses were performed across the case-control, fatal-control, and fatal-nonfatal case groups using Genespring (Ver 3.0). Fifteen miRNAs were statistical significant in patients with CCHF compared with the controls (5 were upregulated, 10 were downregulated). Seventy-five and sixty-six miRNAs are in fatal compared with control and nonfatal case, respectively (fold change ([FC] >= 50) were statistically significant. In this study, the target genes of important miRNAs were identified and Gene Ontology analyses were performed across all groups. As a result of this study, we propose that the detection of miRNAs in patients with CCHF will allow the determination of therapeutic targets in diseases. CCHF is an important public health problem that can often be fatal. In this study, we investigated miRNA expression in case-control, fatal-control, and fatal-nonfatal case groups. Significant miRNAs associated with fatality were detected in CCHF. This study will serve as a source of data for the development of an antagomir-based therapy against CCHF using miRNAs in the future.
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    Oxidative, nitrosative and glycosative stress levels in Crimean-Congo hemorrhagic fever disease
    (Bayrakol Medical Publisher, 2020) Ersan, Serpil; Bakir, Sevtap; Engin, Aynur; Bakir, Mehmet
    Aim: This study aimed to investigate Oxidative/Nitrosative/Glycosative stress (OS/NS/GS) biomarkers levels in CCHF disease, their levels in the course of the disease, and to benefit from the results obtained in the pathogenesis and treatment of the disease. Material and Methods: In the study, serum OS, NS, and GS biomarkers levels of the participants in the CCHF (n = 60) and control (n = 35) groups were compared. In addition, the participants with CCHF were classified as mild, moderate, and severe infection subgroups according to the Severity Grading Score (SGS). A commercial enzyme-linked immunosorbent test kit was used to measure the levels of 8-OHdG, 3-NT, 8-NG, NO, CML, 8-iso-PGF2a in serum samples obtained from the participants in the CCHF and control groups. MDA levels were measured in serum samples by a spectrophotometric method. Total Antioxidant Status and Total Oxidant Status levels were determined using commercial kits. Results: On the whole, the mean OS/NS/GS biomarkers levels in the participants in the CCHF group were significantly higher than were those in the control group (p <0.005). Accordingly, it was found that in the participants with CCHF, as the severity of the disease increased so did the biomarker levels. Discussion: Consequently, in addition to routine laboratory tests, the presence of unbalanced OS/NS/GS in CCHF should be taken into account in the followup of patients. Considering that the main factor in CCHF treatment is supportive therapy, adding antioxidant agents to the treatment can contribute to the improvement of the prognosis.