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Öğe Changes in arginine metabolism in advanced Alzheimer?s patients: Experimental and theoretical analyses(Elsevier, 2023) Saria, Ismail; Ersan, Serpil; Ozmen, Esma; Ayan, Durmus; Ersan, Erdal; Berisha, Avni; Kayae, SavasLimited data obtained in studies conducted in recent years suggest that changes in arginine metabolism may be associated with the pathogenesis of Alzheimer's disease (AD). However, the underlying mecha-nisms of this pathway's effects on the disease are not clear and there are conflicting data. Therefore, in this study, we aimed to determine the levels of L-arginine and its important metabolites and enzymes involved in the pathway in advanced AD patients to examine the change in L-arginine metabolism as inclusively as possible.Serum and plasma samples were obtained from 51 patients diagnosed with advanced AD and 30 volunteer controls. Arginase, Ornithine Decarboxylase (ODC), Arginine Decarboxylase (ADC), and Agmati-nase levels in serum samples were determined by enzyme-linked immunosorbent assay (ELISA) and, L- arginine, Ornithine and nitric oxide (NO) levels were determined by colorimetric method. Agmatine levels were measured by high-performance liquid chromatography in the plasma samples of the study groups. Furthermore, in silico molecular docking studies were performed to get preliminary knowledge about the binding interactions of the agmatine with various targets such as AChE, butyrylcholinesterase (BuChE), BACE-1 and tau protein kinase 1 which play an important role in AD pathogenesis.Agmatine and L-arginine levels were found to be significantly lower in the patient group than in the control group. Milder but not statistically significant reductions were observed in all other param-eters we measured involved in L-arginine metabolism. Furthermore, NO levels were found to be sig-nificantly lower in men with advanced AD patients than in control men. It has been analyzed that agmatine ligand interacts effectively with the studied proteins which play an important role in AD pathogenesis; these interactions were significant and, based on the docking score, occurred in the fol-lowing order: butyrylcholinesterase (PDB id: 1P0I) > Human acetylcholinesterase > Human tau-protein kinase I.In conclusion, in advanced AD patients, the activity of the L-arginine pathway decreased in gen-eral, especially agmatine formation, and this may be due to the decrease in L-arginine levels. Therefore, arginine de novo synthesis may be decreased in advanced AD patients. Furthermore, according to the MolDock binding score, agmatine ligand has a high binding affinity for proteins involved in AD manage-ment and/or pathogenesis. Therefore, agmatine may play a role in the pathogenesis of AD by inhibit-ing the activity of these proteins. However, additional comprehensive studies are needed to clarify these thoughts.(c) 2023 Elsevier B.V. All rights reserved.Öğe Determination of levels of oxidative stress and nitrosative stress in patients with epilepsy(Elsevier, 2020) Ersan, Serpil; Cigdem, Burhanettin; Bakir, Deniz; Dogan, H. OkanBackground: Epilepsy is one of the most common neurological diseases. The underlying pathophysiological mechanisms in epilepsy are still unknown. Oxidative stress is believed to be one of the factors involved in the pathogenesis of epileptogenesis. In various pathophysiological conditions, reactive nitrogen species (RNS) such as nitrogen and peroxynitrite are produced and these RNSs can bind to free nucleosides and nucleotides or to nucleosides and nucleotides existing in the DNA/RNA structure. 8-Nitroguanine (8-NG) is a typical DNA nucleobase product of nitrosative damage generated by RNS. It has been proposed that F2-isoprostanes, in particular 8-iso-Prostaglandin F2a (8-isoPGF2a), are specific, reliable and non-invasive biomarkers of lipid peroxidation in vivo. In the present study, we compared the levels of lipid oxidative stress biomarker 8-isoPGF2a and nitrosative stress DNA biomarker 8-NG in patients with epilepsy undergoing antiepileptic drug (AEDs) treatment and with those in healthy participants. Methods: The present study comprised 90 patients aged between 17 and 53 who were admitted to the Neurology Clinic of Cumhuriyet University and diagnosed with epilepsy. The patients were assigned into the intervention (n = 45) and control (n = 45) groups. Of the participants in the intervention group, 37.7% (n = 17) were treated with levetiracetam (LEV), 33.3% (n = 15) with valproic acid (VA) and 29% (n = 13) with carbamazepine. Serum 8-iso-PGF2a and 8-NG levels of the participants in the intervention and control groups were determined by ELISA. Results: There was no significant difference between the medication (LEV, VA, Carbamazepine) used by the participants and their 8-iso-PGF2a and 8-NG levels (p> 0.05). However, 8-iso-PGF2a and 8-NG were significantly higher in the participants in the intervention than in the participants in the control group (p< 0.001). Conclusion: Our study demonstrated that there was an increase in oxidative and nitrosative stres markers in patients with epilepsy. There was no significant difference between the 8-iso-PGF2a and 8-NG levels of the participants taking three different AEDs.Öğe Effects of agmatine, glutamate, arginine, and nitric oxide on executive functions in children with attention deficit hyperactivity disorder(Springer Wien, 2020) Sari, Seda Aybuke; Ulger, Dilara; Ersan, Serpil; Bakir, Deniz; Uzun Cicek, Ayla; Ismailoglu, FiratIn this study, we aimed to investigate the effects of agmatine, nitric oxide (NO), arginine, and glutamate, which are the metabolites in the polyamine pathway, on the performance of executive functions (EF) in attention deficit hyperactivity disorder (ADHD). The ADHD group included 35 treatment-naive children (6-14 years old) who were ewly diagnosed with ADHD. The control group consisted of 35 healthy children with the same age and sex, having no previous psychiatric disorders. In the study groups, Stroop test (ST) and trail making test (TMT) were used to monitor EF, and blood samples were collected to measure agmatine with ultra-high-performance liquid chromatography and NO, glutamate, and arginine with enzyme-linked immunosorbent assay (ELISA). The EFs were significantly impaired in the ADHD group. The agmatine and arginine levels of the ADHD group were significantly higher than their peers. The NO and glutamate levels were also higher in the ADHD group compared to the control group, but these differences did not reach statistical significance. Children with ADHD had more difficulties during EF tasks compared to healthy children. The elevated NO and glutamate levels may be related with the impairment during EF tasks. Therefore, agmatine and arginine may increase to improve EF tasks through its inhibitory effect on the synthesis of NO and glutamate. Further studies are needed about polyamine pathway molecules to shed light on the pathophysiology of ADHD.Öğe Effects of Hypnotherapy on Weight Loss and thus on Serum Leptin, Adiponectin, and Irisin Levels in Obese Patients(Mary Ann Liebert, Inc, 2020) Ersan, Serpil; Ersan, Etem ErdalObjective:This study aimed to investigate effects of hypnotherapy on weight loss and thus on leptin, adiponectin (ADP), and irisin levels in obese patients who presented to the psychiatry outpatient clinic to participate in hypnotherapy sessions to develop a healthy and balanced diet. Methods:The study sample included 32 individuals with a body mass index (BMI) of >= 30 and who completed the 10-week hypnotherapy. First, the Personal Information Form was handed out to the participants and then each participant's weight (in the morning on an empty stomach) and height were measured and BMI was determined. Five-milliliter blood samples were drawn before the first session, and then irisin, leptin, and ADP levels were measured using the enzyme-linked immunosorbent assay method. Then, they participated in hypnotherapy sessions once a week for 10 weeks. At the end of the 10-week hypnotherapy, 5 mL of blood was taken again and the aforementioned biochemical analyses were performed. BMIs were measured again. Results:The mean BMI values of the patients were 33.43 +/- 5.28 and 31.45 +/- 4.98 at the beginning and end of the hypnotherapy sessions, respectively. Serum leptin, ADP, and irisin levels, which were 9.48 +/- 5.48, 6.73 +/- 3.27, and 1.43 +/- 1.14, respectively, at the beginning of the hypnotherapy sessions, were 6.47 +/- 2.69, 7.68 +/- 3.34, and 1.53 +/- 1.21, respectively, at the end of the hypnotherapy sessions. The results showed that BMI and serum leptin levels decreased significantly after the hypnotherapy sessions, whereas serum ADP and irisin levels increased significantly. Conclusions:This study indicates that hypnotherapy in obesity treatment leads to weight loss in obese patients and thus to considerable changes in leptin, ADP, and irisin levels. Hypnotherapy is easy to apply, cheap, and effective; has no potential for side effects; and can be applied both alone and in combination with other treatments. However, to confirm its effects, further studies should be conducted on this issue.Öğe Evaluation of Glucagon-like Peptide-1, Adropin, and Desnutrin Levels and Related Factors in Patients with Bipolar Disorder(Aves, 2021) Ersan, Serpil; Kurt, AydinObjective: It is known that impaired energy metabolism contributes to the neuropathology of bipolar disorder (BD). This study aimed to compare the levels of glucagon-like peptide-1 (GLP-1), adropin, and desnutrin, which have many metabolic functions besides the regulation of energy metabolism, between patients with BD and healthy controls and to investigate the related factors. Methods: In the study group, 73 age- and sex-matched participants were included. Of them, 35 were patients diagnosed with BD and 38 were healthy individuals. In the blood samples, in addition to routine biochemistry lipid parameters, the levels of adropin, desnutrin, and GLP-1 were determined. Results: Adropin, desnutrin, and GLP-1 levels were significantly lower in patients with BD than in healthy controls (P < 0.001). In contrast, body mass index, total cholesterol, triglyceride, and low-density lipoprotein levels were significantly higher in patients with BD than in healthy controls (respectively P < 0.001, P= 0.001, P= 0.002, P= 0.001). It was observed that adropin levels decreased significantly as the duration of the disease increased. Conclusion: The low levels of adropin, desnutrin, and GLP-1 that we determined in patients with BD indicate that these peptides may be important in BD pathophysiology.Öğe EVALUATION OF OXIDATIVE STRESS, 3-NITROTYROSINE, AND HMGB-1 LEVELS IN PATIENTS WITH WET TYPE AGE-RELATED MACULAR DEGENERATION(Soc Medical Biochemists Serbia, 2022) Zor, Kursad Ramazan; Sari, Ismail; Bicer, Gamze Yildirim; Gunturk, Inayet; Kucuk, Erkut; Ersan, Serpil; Seydel, Gonul SeydaBackground: This study aims to compare serum HMGB-1, 3-nitrotyrosine (3-NT), TAS, TOS, and OSI levels in Wettype Age-Related Macular Degeneration (wAMD) patients and healthy controls to determine the correlation of these parameters with each other. Methods: Thirty patients with Wet-type Age-Related Macular Degeneration (wAMD) and 27 healthy adults, as controls were enrolled in the study. We determined the TAS and TOS levels in serum samples of both groups using commercial kits on a microplate reader. Serum HMGB-1 and 3-NT levels were measured with the enzyme-linked immunosorbent assay method. Results: HMGB-1 levels were significantly higher in the patient group (137.51 pg/mL, p=0.001), while there was no difference between the two groups in serum 3-NT levels (p = 0.428). A statistically significant difference found in the levels of TOS and OSI (p = 0.001 and p = 0.045, respectively) between the patients and controls, however, no significant difference was observed between the groups in terms of TAS levels (p = 0.228). Conclusions: Oxidative stress and HMGB-1 levels were increased in wAMD patients and enhanced oxidative stress may be associated with increased tissue necrosis and inflammation. Thus administration of antioxidant treatment in addition to routine therapy should be considered in wAMD.Öğe Oxidative, nitrosative and glycosative stress levels in Crimean-Congo hemorrhagic fever disease(Bayrakol Medical Publisher, 2020) Ersan, Serpil; Bakir, Sevtap; Engin, Aynur; Bakir, MehmetAim: This study aimed to investigate Oxidative/Nitrosative/Glycosative stress (OS/NS/GS) biomarkers levels in CCHF disease, their levels in the course of the disease, and to benefit from the results obtained in the pathogenesis and treatment of the disease. Material and Methods: In the study, serum OS, NS, and GS biomarkers levels of the participants in the CCHF (n = 60) and control (n = 35) groups were compared. In addition, the participants with CCHF were classified as mild, moderate, and severe infection subgroups according to the Severity Grading Score (SGS). A commercial enzyme-linked immunosorbent test kit was used to measure the levels of 8-OHdG, 3-NT, 8-NG, NO, CML, 8-iso-PGF2a in serum samples obtained from the participants in the CCHF and control groups. MDA levels were measured in serum samples by a spectrophotometric method. Total Antioxidant Status and Total Oxidant Status levels were determined using commercial kits. Results: On the whole, the mean OS/NS/GS biomarkers levels in the participants in the CCHF group were significantly higher than were those in the control group (p <0.005). Accordingly, it was found that in the participants with CCHF, as the severity of the disease increased so did the biomarker levels. Discussion: Consequently, in addition to routine laboratory tests, the presence of unbalanced OS/NS/GS in CCHF should be taken into account in the followup of patients. Considering that the main factor in CCHF treatment is supportive therapy, adding antioxidant agents to the treatment can contribute to the improvement of the prognosis.Öğe Relationship between chronic obstructive pulmonary disease and telomerase, agmatine, deubiquitinase and sirtuin in the pathway of aging(Bayrakol Medical Publisher, 2023) Kurucay, Deniz; Ozmen, Zeliha Cansel; Seyfikli, Zehra; Ersan, SerpilAim: Chronic Obstructive Pulmonary Disease (COPD) is a progressive disease that has both chronic bronchitis-related changes in airways and differences of emphysema in the lung parenchyma. Aging is a complex and irreversible process involving cells, tissues, organs and systems resulting from numerous endogenous and exogenous factors. Agmatine, telomerase, deubiquitinase and sirtuin molecules appear during aging process. A better understanding of the role of these molecules in aging process may lead to advancing therapies for COPD and comorbidities.In the present study, it was aimed to investigate whether there was a relationship between COPD, a chronic progressive disease, and agmatine, telomerase, sirtuin and deubiquitinase, which are molecules involved in the aging pathway.Material and Methods: Thirty-five patients with COPD and 35 healthy persons without chronic diseases participated in this study. Serum agmatine levels were measured by a fluorescence detector using a high-performance liquid chromatography method (HPLC). Serum Sirtuin, deuibiquitinase and telomerase levels were performed using the ELISA method.Results: A result of the statical analysis in the terms of the deubiquitinase showed no differences between patients and control groups (p>0.05). In the patient group, sirtuin values were lower than in controls (P<005). Telomerase and agmatine values were higher (P<0.05).Discussion: As a result, the increase in telomerase activity may be to prolong the telomere shortening in COPD patients. The increase in agmatine may also be caused by depression and hypoxia in COPD. Decreased sirtuin in COPD can be explained by the increase in aging and inflammation.Öğe Serum Irisin Levels and Its Relationship with Spasticity Severity in Chronic Stroke Patients(Thieme Medical Publ Inc, 2022) Kaydok, Ercan; Sari, Ismail; Ersan, Serpil; Kucuk, Esin BenliObjective Irisin is a myokine released from muscles by exercise and it has been shown to be a prognostic indicator in acute stroke patients. However, irisin's relationship with the chronic phase of stroke and spasticity has not been studied yet. We aimed to determine the serum level of irisin to examine its relationship with the functional status and severity of spasticity in patients with chronic stroke, and to compare these with healthy controls. Materials and Methods A total of 70 (35 chronic stroke and 35 control patients) patients were included in the study. The blood serum irisin levels of the patients and the controls were evaluated using enzyme-linked immunosorbent assay method, their functional status was evaluated with the modified Rankin scale (mRS), and spasticity severity using the modified Ashworth scale (MAS). Results The mean serum irisin levels of the stroke and the control groups were 6.20 +/- 2.2 and 5.45 +/- 2.3, respectively, and there was no statistically significant difference ( p > 0.05). No significant correlation was found between the serum level of irisin and the severity of spasticity and functional status, assessed by the mRS in stroke patients. Conclusion These results showed that irisin levels in chronic stroke patients were similar to controls, and there was no relationship between the severity of spasticity and functional status and irisin level.Öğe Serum malondialdehyde, monocyte chemoattractant protein-1, and vitamin C levels in wet type age-related macular degeneration patients(Sage Publications Ltd, 2020) Zor, Ramazan Kursad; Ersan, Serpil; Kucuk, Erkut; Yildirim, Gamze; Sari, IsmailPurpose: The purpose of this study was to investigate the serum levels of malondialdehyde (MDA) which is a marker of oxidative stress, monocyte chemoattractant protein-1 (MCP-1) which has an important role in inflammation, and vitamin C which has antioxidant properties in patients with wet age-related macular degeneration (wAMD). Methods: Thirty patients with wAMD were included in the study and serum levels of MDA, MCP-1, and vitamin C were compared with healthy participants (n = 30). Serum vitamin C and MDA levels were measured using a spectrophotometric method. Serum MCP-1 levels were determined by the ELISA method. Results: MCP-1 and MDA levels were higher in patients with wAMD compared with the control group (p < 0.05). Serum vitamin C levels were lower in patients with wAMD compared with the control group (p < 0.05). Conclusions: The increase in the MCP-1 levels in patients with wAMD may be associated with increased inflammation in wAMD. Decreased serum vitamin C and elevated MDA levels in patients with wAMD suggest increased oxidative stress in wAMD patients. These results indicate that the increased oxidative stress and inflammation can play a role in the pathogenesis of wAMD.Öğe The effect of acute step-aerobic exercise on adiponectin and leptin levels in premenopausal women(Edizioni Minerva Medica, 2021) Sari, Ismail; Habipoglu, Sedef; Seydel, G. Seyda; Ersan, Serpil; Gunturk, InayetBACKGROUND: It is well known that the prevalence of obesity, cardiovascular disease and metabolic syndrome is increasing during the transition to menopausal stages. The aim of this study was to investigate the effects of acute step-aerobic exercise on levels of leptin and adiponectin, which are adipose tissue-derived adipocytokines, associated with obesity, hypertension and other diseases in sedentary premenopausal women. METHODS: Twenty-four sedentary premenopausal volunteer women between the ages of 40-45 (premenopause group) and 24 healthy adults between the ages of 21-39 (control group) were enrolled in this study. Moderate intensity step-aerobic exercise (50-60% of HRmax) was applied for 60 min to the premenopausal subjects. Venous blood samples were obtained before and after the exercise. Adiponectin and leptin levels were determined using the ELISA method. RESULTS: No statistically significant difference was found in terms of BMI values, and leptin levels between the premenopause and control group, while differences of adiponectin were statistically significant. Plasma leptin levels of the premenopausal women decreased whereas adiponectin levels increased meaningfully after the acute aerobic exercise (P<0.05). When the premenopausal women were grouped according to BMI, there were no significant differences between BMI <= 30 and >30 groups in terms of leptin and adiponectin levels measured before and after exercise. CONCLUSIONS: Our results may suggest that acute step aerobic exercise in premenopausal women alters leptin and adiponectin levels in favor of the organism. However, further studies are needed.Öğe The impact of Dicer, Drosha, and Exportin-5 levels in polycystic ovary syndrome (PCOS) diagnosis and phenotyping(Via Medica, 2022) Duman, Gulhan; Sariakcali, Baris; Ersan, Serpil; Bakir, SevtapIntroduction: Polycystic ovary syndrome (PCOS) is a very common heterogeneous endocrine and gynaecological disease in reproductive women. Early identification and treatment of patients are necessary to prevent future cardiometabolic and reproductive complications. In our study, we aimed to investigate whether Drosha, Exportin-5 (XPO5), and Dicer, which are involved in miRNA formation, are useful markers in the diagnosis of the disease. Material and methods: Patients who presented to our clinic with complaints such as menstrual irregularity, hirsutism, and acne were diagnosed with polycystic ovary after excluding other possible diagnoses, and if they meet two-thirds of the Rotterdam diagnostic criteria, they were included in the study. Thirty patients with polycystic ovaries and 35 healthy controls were included in this study. Results: The mean values of XPO5, Drosha, and Dicer markers were significantly higher in the PCOS group when compared with the control group. With an XPO5 value > 1.70, we found the PCOS with 94% probability, 86.7% sensitivity, and 91.4% specificity. Moreover, if the Drosha value was > 0.166, it was expected that the patient would be diagnosed as PCOS with a probability of 75%, with 66.7% sensitivity and 71.4% specifidty. A statistically significant cut-off value could not be obtained for Dicer. Conclusions: In our study, the levels of all three markers were found to be significantly higher in the PCOS group compared to the control group. It suggests that they can be used in the early diagnosis of PCOS patients without full-blown disease. However, this preliminary study should be supported by larger-scale studies.
