Yazar "Evlice, Emre" seçeneğine göre listele
Listeleniyor 1 - 7 / 7
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Characterization of Potato Golden Cyst Nematode Populations (Globodera rostochiensis) in Turkey(Friends Science Publ, 2020) Toktay, Halil; Evlice, Emre; Imren, Mustafa; Ozer, Goksel; Ali, Muhammad Amjad; Dababat, AbdelfattahGolden potato cyst nematode, Globodera rostochiensis (Wollenweber) Behrens, is one of the most important soilborne pathogens causing economic losses in potato. The nematode is known to occurin several countries including Turkey and has a worldwide regulatory concern. In this study, identification and genetic diversity of G. rostochiensis specimens obtained from the main potato producing areas of Turkey were determined. Twenty -five of 35 soil samples collected from the provinces of Izmir, Nevsehir and Nigde were found to contain G. rostochiensis. The variation between G. rostochiensis populations was determined when examined according to ecological and pathogenic characteristics of nematode in Turkey. The cysts of G. rostochiensis were identified by measuring the morphological characters using perennial patterns, vulval cone, vulval basin and juveniles. Phylogenetic analysis of the Large Sub Unit (LSU) region of rDNA sequences was used to assess the inter or intra phylogenetic relationships between the nematode populations. The phylogenetic analyses demonstrated that the nematode specimens from Turkey cluster with Globodera spp. and signified the presence of single species of G. rostochiensis. As a result, morphological, morphometric and molecular methods were successfully combined for identification and characterization of G. rostochiensis. The frequency of G. rostochiensis in regulatory samples frompotato -producing areas is becoming increasingly important. The morphological characterization has several complications in the detection of this quarantine nematode, using of this combination is beneficial for a reliable and quick diagnostic for these nematodes which is crucial for regulatory services and growers. The results might help to investigate different ecotypes of G. rostochiensis for comprehensive understanding about physiology, ecology , and biology of the genus Globodera for its effective management in Turkey. (C) 2020 Friends Science PublishersÖğe molecular characterization of Heterodera cruciferae Franklin, 1945 from cabbage felds in Nigde province, Turkey (Sep, 10.1007/s11033-022-07860-w, 2022)(Springer, 2023) Toktay, Halil; G. Akyol, Badel; Evlice, Emre; Imren, Mustafa[Abstract Not Available]Öğe Molecular characterization of Heterodera cruciferae Franklin, 1945 from cabbage fields in Nigde province, Turkey(Springer, 2022) Toktay, Halil; Akyol, Badel G.; Evlice, Emre; Imren, MustafaBackground The aim of this study was to identified cyst nematodes in the cabbage production areas in Nigde Province by molecular methods to underpin decision making for field control. The sequences of ribosomal DNA region (rDNA-ITS) and cytochrome oxidase subunit 1 (mtDNA-COI) were used for the first time for identification Heterodera cruciferae (cabbage cyst nematode) in Turkey. Methods and Results Heterodera cruciferae populations extracted from cabbage growing areas of Nigde Province and investigated using sequences of rDNA-ITS and mtDNA-COI. Similarities and differences between 13 geographic populations of H. cruciferae were detected in surveys during 2020-2021. DNA from single cysts was successfully amplified, and genetic variability was revealed within nematode populations. Based on these results, H. cruciferae is reported for the first time from Nigde Province, Turkey. This study showed clear discrimination among the sampled populations of H. cruciferae. Conclusion This finding is important for control and managing cabbage cyst nematode in cabbage fields in Turkey as more than one Heterodera spp. can occur. Future studies should investigate the population dynamics and control of H. cruciferae in fields in the sampled districts.Öğe Phytophagous nematodes in cereal fields in Nigde Province, Turkey(Entomological Soc Turkey, Ege Univ, 2020) Toktay, Halil; Imren, Mustafa; Akyol, Badel G.; Evlice, Emre; Riley, Ian T.; Dababat, Abdelfattah A.This study evaluated the occurrence and incidence of phytophagous nematodes and identified the cereal cyst nematode species by morphological and molecular tools in the main cereal-growing areas in Nigde in 2018-2019. Phytophagous nematodes within twelve genera were detected in 95% of soil samples. The most common phytophagous nematodes in cereal soil were in the genera Heterodera, Ditylenchus, Merlinius, Pratylenchus, Aphelenchus, Aphelenchoides, Tylenchus, Helicotylenchus, Trophurus, Pratylenchoides, Filenchus and Xiphinema (in decreasing order of incidence). In particular, 75% of the soil samples from surveyed fields were infested with the cereal cyst nematodes (Heterodera spp.). Morphological characteristics of cysts and second-stage juveniles were calculated within the expected ranges for Heterodera filipjevi (Madzhidov, 1981) Stelter, 1984, however, two populations from Camardi was determined as Heterodera latipons Franklin, 1969 (Tylenchida: Heteroderidae). Intraspecific variation was not observed within the populations of H. filipjevi which could be in the same genotypic group. In addition to the high incidence of these Heterodera spp., intensive cereal cropping systems with/without non-cereal rotations in wheat production areas of Nigde also resulted in high incidence of root lesion nematode, Pratylenchus species.Öğe Plant Parasitic and Virus Vector Nematodes Associated with Vineyards in the Central Anatolia Region of Turkey(ZOOLOGICAL SOC PAKISTAN, 2014) Kepenekci, Ilker; Toktay, Halil; Evlice, EmreGrapevines is host for many plant parasitic nematode species and virus transmission species. Surveys were undertaken in 15 vineyards in Turkey. Samples were taken from each vineyard older than 10 years, from depth of 0-30 cm and 30-60 cm. Totally 300 subsamples were examined. In this study, 22 species were identified belonging to 16 genus within Tylenchida, Aphelenchida, Dorylaimida and Triplonchida orders from soil and root samples of vine (Vitis vinifera L.) in the Central Anatolia Region (Nevsehir, Karaman, Konya, Isparta and Burdur provinces). Malenchus fusiformis, Aphelenchoides clarus, A. confusus, Rotylenchus (R.) colbrani, Xiphinema diversicaudatum and Trichodorus similis were found for the first time in Turkey. The species most frequently encountered were X pachtaicum and Helicotylenchus crenacauda.Öğe Population fluctuations of root-knot nematodes Meloidogyne chitwoodi and M. hapla under field conditions(Springer, 2022) Evlice, Emre; Toktay, Halil; Yatkin, Gokhan; Erdogus, Fatma Dolunay; Imren, MustafaMeloidogyne chitwoodi Golden, O'Bannon, Santo & Finley, 1980 and M. hapla Chitwood, 1949 are important pests of potato in Turkey. This study was conducted in three experimental areas where conventional agronomic practices have been done in Nigde (Center district) and Nevsehir (Center and Derinkuyu districts) provinces. Nematodes were extracted by modified Oostenbrink dish from soil samples taken from 0 to 30 cm depths every month between 2014 and 2017 to assess the population fluctuations of these root-knot nematodes under field conditions. The whole suspension was checked for Meloidogyne juveniles which were counted under an inverted microscope. Morphological and molecular identification results showed that two experimental areas were infested by M. chitwoodi while one experimental area (Derinkuyu) was infested with both M. chitwoodi and M. hapla. Our study showed that M. chitwoodi population level decreased during wheat cultivation and were maintained under rye cultivation, whereas M. chitwoodi and M. hapla population levels increased rapidly under potato and bean cultivation. It was found that M. chitwoodi population increased more when potatoes were planted, while M. hapla increased more in bean. The best sampling time for M. chitwoodi and M. hapla was observed to be right after harvest, when the population was highest, but both could be detected in soil samples even when the ambient temperature was below zero. These results demonstrated that farmers should be careful in their choice of rotational crop with potatoes in areas infested with M. chitwoodi and M. hapla.Öğe Türkiye'deki Altın Kist Nematodu (Globodera rostochiensis) (Tylenchida: Heteroderidae) Popülasyonlarının Morfolojik ve Morfometrik Karakterizasyonu(2021) Evlice, Emre; Yatkın, Gökhan; Öcal, Atilla; Güvel, Civan; Toktay, HalilPatates kist nematodları (PKN) Altın kist nematodu olarak isimlendirilen Globodera rostochiensis(Wollenweber, 1923) Skarbilovich, 1959 ve Beyaz kist nematodu olarak isimlendirilen G. pallida Stone, 1973(Tylenchida: Heteroderidae), tüm dünyada hemen hemen tüm patates alanlarında önemli verim kayıplarınaneden olan yaygın türlerdir. Önemli karantina etmenleri olan PKN’larının doğru ve hızlı teşhisi mücadelesiaçısından çok önemlidir. Bu iki türün oluşturduğu risk nedeniyle, Türkiye'de bu etmenlerin varlığı sertifikalı veyemeklik patates alanlarında ulusal survey programı kapsamında taranmaktadır. Çalışma kapsamında Niğde,Nevşehir, İzmir, Afyon ve Bolu illerine ait 10 farklı lokasyondan elde edilen PKN popülasyonlarına ait kist veikinci dönem larvalar morfolojik ve morfometrik karakterlerine göre teşhis edilmiştir. Çalışma sonucundapopülasyonların tamamı G. rostochiensis olarak teşhis edilmiştir. Türkiye’deki bulaşık alanların tamamınıkapsayacak şekilde ilk kez yapılan bu çalışmayla ülkemizdeki G. rostochiensis popülasyonlarının morfolojikolarak teşhisinin yapılabildiği, ölçüm değerlerinin referans literatürlerle uyumlu olduğu belirlenmiştir. Ancakrutin analizlerde tüm dünyada en önemli karantina etmenlerinden biri olan Patates kist nematodlarınınteşhisinde morfolojik ve moleküler yöntemlerinin bir arada kullanılmasının daha doğru olacağıdüşünülmektedir.
