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Öğe Biochemical and Histopathological Investigation of the Protective Effect of Lutein in Rat Kidney Exposed to Cisplatin(2022) Bilgiç, Sedat; Gür, Fatih Mehmet; Aktaş, İbrahimAim: The aim of this study was to determine the protection of lutein (L) (100 mg/kg, orally) against kidney damage caused by cisplatin (Cis) (10mg/kg, intraperitoneal). Materials and Methods: Twenty-eight rats aged 8 weeks were used and divided into four equal groups (n=7): Control, L, Cis, and Cis + L. Results: As a result of biochemical findings, serum Cr and BUN levels of the Cis group were significantly increased compared to the control group (pÖğe Cüce Hamsterlerin (Phodopus roborovskii) Genital Dokularında Androjen Reseptör Lokalizasyonu(2018) Gür, Fatih Mehmet; Timurkaan, Sema; Baygeldi, Saime Betül; Özkan, Zait Ender; Kanmaz, Yeşim Aslan; Gençer, Berrin Tarakçı; İlgün, RamazanAndrojenler hedef dokulardaki etkilerini esas olarak androjen reseptör (AR) aracılığıylagösterirler. Bu çalışmada dişi ve erkek cüce hamster genital dokularında ARlokalizasyonu araştırıldı. Mevcut çalışmada 6 adet dişi, 6 adet erkek hayvan kullanıldı.Anestezi sonrası, çalışılacak dokular, hızlı bir şekilde vücuttan uzaklaştırılarak %10’luktamponlu nötral formalinde tespitin ardından parafine gömüldü. Kesitler, mikrodalgaışınımlı “antijen retrieval” tekniği uygulandıktan sonra immunohistokimyasalyöntemlerle boyandı. AR pozitif immunboyanma testis, kaput epididimis, prostat bezi vevezikula seminalis dokularında yalnızca hücre çekirdeklerinde gözlenirken, ovaryum veovidukt dokularında hücrelerin bir kısmında sitoplazmada bir kısmında ise çekirdektegözlendi. Cüce hamster erkek ve dişi genital dokularındaki AR lokalizasyonu, diğertürlerle benzerdi. Genital dokularda AR varlığı, androjenlerin bu dokular için elzemolduğu görüşünü desteklemektedir.Öğe Immunoexpression of androgen receptor in genital tissues of male BALB/c mice(2022) Gür, Fatih MehmetAim: Androgens are hormones that are essential for the differentiation and development of male genital organs, preserving their structural features and maintaining their functions. These hormones exert their effects on target tissues mainly through androgen receptors (AR). The aim of this study was to determine the AR immunoexpression pattern in the genital tissues of male mice. Materials and Methods: A total of 6 BALB/c male mice were anesthetized with ether, and the genital tissues, which were quickly removed from the body, were fixed in Bouin’s solution for 18 hours. Tissues that underwent routine histological procedures after fixation were embedded in paraffin. Tissue sections cut with a thickness of 5 ?m from paraffin blocks were taken on a slide and examined by staining with immunohistochemical methods. Results: AR-positive immunostaining in the testis was observed only in somatic cells such as Sertoli, peritubular myoid cells, and Leydig cells. Germ cells were AR-negative. In the caput epididymis, ductus deferens, and seminal vesicle, AR positive immunoexpression was observed in stromal cells, especially epithelial cells. Although AR-positive staining was observed in some of the epithelial and stromal cells in these organs, some of them were AR-negative. Conclusion: In the current study, it was determined that the AR expression pattern in the genital tissues of male BALB/c mice was similar to that in other species, although there were minor differences. The findings support the hypothesis that androgens exert their effects in testis mainly through somatic cells, and their effects in other genital tissues through epithelial cells, and the fact that androgens/ARs are essential for maintaining the structures and functions of male genital organs.Öğe Investigation of the potential teratogenic effects of fructose on the embryo using the rat whole embryo culture model(Elsevier Ltd, 2024) Gür, Hatice Emel; Balcıoğlu, Esra; Patat, Dilara; Uçar, Sümeyye; Gür, Fatih Mehmet; Yalçın, Betül; Nisari, MehtapExcessive consumption of fructose-sweetened foods and beverages is a growing concern worldwide. Studies have demonstrated that fructose consumption before and during pregnancy can result in adverse outcomes such as decreased decidualization, increased fetal losses, and low birth weight. The study investigated the teratogenic effects of fructose on rat embryos during organogenesis using whole embryo culture. Within the scope of the study, 4 groups were formed as control, low, medium, and high-dose fructose (HDF) with 10 embryos in each group. The 9.5-day-old rat embryos were cultured with different concentrations of fructose (1, 5 and 10 mM) for 48 h and the possible effects of fructose were examined using morphological scoring, histochemistry, immunofluorescence, and TUNEL methods. According to the analyses, protein synthesis and proliferation were decreased, vascular formation was suppressed, and apoptosis was increased in embryos exposed to fructose, especially at concentrations of 5 mM and above. According to the morphological scoring results, it was determined that heart, hind limb, and somite development were retarded in all experimental groups compared to the control group, developmental retardation increased in direct proportion to fructose concentration, and also significant malformations were observed in all parameters examined in the HDF group. In addition, analysis of yolk sac diameter, head length, crown rump length and somite numbers showed that these parameters were significantly decreased in all experimental groups. End of the study, it was concluded that fructose at concentrations of 1 mM and above may induce embryonic development retardation and other anomalies by decreasing protein synthesis and cell proliferation, suppressing vascular formation, and increasing apoptosis in embryonic tissues. © 2024 Elsevier LtdÖğe Macro-anatomical and Morphometric Investigation of the Tongue and Lingual Papillae in the Guinea fowl (Numida meleagridis)#(2019) İlgün, Ramazan; Kuru, Nilgün; Bölükbaş, Ferhan; Gür, Fatih MehmetThe aim of this study was to investigate the macroanatomy and morphometric of the tongue of theguinea fowl. Six chicks (6-7 weeks), six layer hens (9-13 weeks) and six studs guinea fowl wereused to study and determine the anatomical features of the tongue of the guinea fowl. Papillae of thetongue were examined photos of the general anatomic structures were taken. Morphometry wascalculated by statistical analysis. The tongue was triangular shaped, and consisted of apex, corpus,and radix sections. The papillae linguales caudales were on both sides of the corpus and radixlinguae. Conical papillae were found to be sequentially V shaped at the radix. Thus, in this study,the anatomy and morphometric of the tongue of the guinea fowl tongue were examined in detailsand the similarities and differences between the tongue of the guinea fowl and the tongue of other poultry species were investigated.Öğe Silimarin’in Paklitaksel Kaynaklı Nefrotoksisiteye Karşı Koruyucu Etkileri(2022) Gür, Fatih Mehmet; Aktaş, İbrahimKanser tedavisinde kullanılan ilaçların etkileri yalnızca kanser hücrelerine özgü olmayıp sağlıklı\rhücreleri de olumsuz etkilemektedir. Bu çalışmada antikanser bir ilaç olan paklitaksel’in (PAX)\rböbrek dokusundaki olası zararlı etkilerine karşı antioksidan etkileriyle öne çıkan silimarin’in (SLY)\rolası koruyucu etkilerinin ortaya konulması amaçlandı. Toplam 28 adet Sprague-Dawley cinsi dişi\rsıçandan rastgele bir seçimle: Kontrol, PAX, SLY ve PAX + SLY olmak üzere dört grup (n=7)\roluşturuldu. PAX grubuna 2 mg/kg intraperitoneal yolla PAX, SLY grubuna oral gavaj sonda ile 100\rmg/kg SLY, PAX + SLY grubuna ise önceki gruplarla aynı doz ve yolla PAX ve SLY uygulandı.\rDeneysel işlemlerin sonunda hayvanlardan alınan kan ve böbrek dokularında yapılan biyokimyasal\ranalizlerde PAX’ın, böbrek dokusunda oksidatif stresi, serumda ise kreatinin (Cr) ve kan üre azotu\r(blood urea nitrogen, BUN) seviyelerini artırdığı tespit edildi. Böbrek dokusunda yapılan\rhistopatolojik incelemelerde PAX’ın renal korpüskül atrofisi, fırçamsı kenarda hasar, vakuolar\rdejenerasyon ve deskuamasyon gibi patolojik değişikliklere yol açtığı gözlendi. Antienflamatuar,\rantiapoptotik ve antioksidan etkilere sahip bir farmakolojik ajan olan SLY uygulanması ise, PAX\rkullanımı sonucu meydana bu gelen patolojik değişiklikleri büyük oranda engelledi. Mevcut\rçalışmada elde edilen sonuçlar PAX ile yapılacak tedavilerde böbrek dokusunda şekillenecek zararlı\retkilere karşı koruyucu olarak SLY’nin kullanılabileceğini gösterdi.Öğe The localization of ER? and ERß in rat testis and epididymis(2020) Gür, Fatih Mehmet; Aktaş, İbrahimAim: It is an indisputable fact that estrogens are essential for the normal functioning of the male reproductive system. Although there are many studies investigating the localization of estrogen receptor ? (ER?) and estrogen receptor ß (ERß) in the testis and epididymis, there is no consensus between the results of these studies. Therefore, in this study, it was aimed to investigate the ER? and ERß expression pattern in testis and epididymis using immunohistochemical methods. Material and Methods: The testis and epididymis tissues removed from 3 adult rats after anesthetized with ether. Then the tissues fixed in Bouin's fixative. The tissues embedded in paraffin were cut 5 ?m thickness with microtomeand stained with immunohistochemical methods. Results: In the testis, ER?-positive immunostaining was observed in the nuclei of peritubular myoid cells and in the cytoplasm of Leydig cells. The germ cells other than spermatogonium and spermatid were ER?-negative. ER?-positive immunoreaction was detected in the nuclei of spermatogonia, peritubular myoid cells and Leydig cells. The cells in which ER? and ER? positive immunostaining were seen most intensively were spermatogonia. ER (? and ß) positive immunostaining in the epididymis was observed in epithelial cells and interstitial stromal cells. Although most of the epithelial cells (principal, basal, apical) were ER? and ER ? positive staining, others were negative.Conclusion: The results obtained in this study showed that ER? and ERß are localized in somatic and germ cells in the testis and epithelial and stromal cells in the epididymis. In this study, it was observed that ER? and ERß staining intensity in spermatogoniums was higher than other cells. This result can be interpreted that estrogens perform their effects on the testis mainly through spermatogonia. The presence of ER (? and ß) in testis and epididymis, support the view that estrogens play an important role in the development and maintenance of male reproductive functions and fertility.
