Yazar "Gazel M." seçeneğine göre listele

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    Evaluation of the susceptibility of different Prunus rootstocks to natural infection of plum pox virus-T
    (2013) Caglayan K.; Serce C.U.; Gazel M.; Kaya K.; Cengiz F.C.; Vidal E.; Cambra M.
    Plum pox virus (PPV) has been observed in Turkey since 1968, but was not widespread except in apricot and plum trees in home gardens and ornamental parks in restricted areas. Susceptibility of six different Prunus rootstocks to strain PPV-T was assessed under natural inoculum pressure in the Izmir-Aegean region during 2010-2011. Aphid populations were monitored from the first week of April to the middle of June by the stickyplant method one year after the rootstock plantation was established. Aphids collected from different rootstocks were tested individually by squash real-time RT-PCR and all rootstocks were regularly tested by DASI-ELISA. The largest aphid populations were observed at the end of May and the most abundant aphid species as averages over the two years were Myzus persicae (20.15%), Hyalopterus pruni (18.64%), Aphis craccivora (9.04%) and Aphis gossypii (8.36%). In 2011, the highest percentage of viruliferous aphids was found in M. persicae (34.78%), followed by H. pruni (32.50%), Macrosiphum euphorbiae (25.00%), A. gossypii (23.80%), A. spiraecola (12.50%) and A. craccivora (10.00%). Of the six Prunus rootstocks tested, only Nemaguard and Myrobalan 29C were infected by PPV-T, infection rate in 2010 being 6.0% (Nemaguard) and 4.0% (Myrobalan 29C). The infection rate increased to 16.0% for Nemaguard and 14.0% for Myrobalan 29C in 2011. However, the other rootstocks, Prunus marianna GF8.1, Docera6, GF677 and Garnem tested negative for PPV-T throughout 2011. PPV isolates obtained from naturally infected apricot trees (inoculum source) and from infected rootstocks in the experimental plot were characterized as PPV-T and had more than 99.5% nucleotide sequence identity.
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    Identification and characterization of a novel Robigovirus species from sweet cherry in turkey
    (MDPI AG, 2019) Çağlayan K.; Roumi V.; Gazel M.; Elçi E.; Acioğlu M.; Plesko I.M.; Massart S.
    High throughput sequencing of total RNA isolated from symptomatic leaves of a sweet cherry tree (Prunus avium cv. 0900 Ziraat) from Turkey identified a new member of the genus Robigovirus designated cherry virus Turkey (CVTR). The presence of the virus was confirmed by electron microscopy and overlapping RT-PCR for sequencing its whole-genome. The virus has a ssRNA genome of 8464 nucleotides which encodes five open reading frames (ORFs) and comprises two non-coding regions, 5' UTR and 3' UTR of 97 and 296 nt, respectively. Compared to the five most closely related robigoviruses, RdRp, TGB1, TGB2, TGB3 and CP share amino acid identities ranging from 43–53%, 44–60%, 39–43%, 38–44% and 45–50%, respectively. Unlike the four cherry robigoviruses, CVTR lacks ORFs 2a and 5a. Its genome organization is therefore more similar to African oil palm ringspot virus (AOPRV). Using specific primers, the presence of CVTR was confirmed in 15 sweet cherries and two sour cherries out of 156 tested samples collected from three regions in Turkey. Among them, five samples were showing slight chlorotic symptoms on the leaves. It seems that CVTR infects cherry trees with or without eliciting obvious symptoms, but these data should be confirmed by bioassays in woody and possible herbaceous hosts in future studies. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
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    Incidence, distribution and limited genetic variability among Turkish isolates of Grapevine Pinot gris virus from different grapevine cultivars
    (Springer Berlin Heidelberg, 2018) Elçi E.; Gazel M.; Roumi V.; Çağlayan K.
    Grapevine Pinot gris virus (GPGV) was firstly identified in northern Italy by deep sequencing from grapevine cv. Pinot gris, exhibiting mottling and deformation of the leaves. The objective of this study was to investigate the prevalence and genetic variability of GPGV isolates obtained from different local and imported grapevine cultivars in Turkey based on partial coat protein, movement protein and RNA-dependent RNA polymerase (RdRp) domain of the replicase (Rep) gene. Two hundred and one grapevine samples from different provinces were tested by RT-PCR assays, approximately 25% of which were found to be infected by GPGV. The PCR products were sequenced and based on the phylogenetic analysis, RdRp gene was found to be most conserved region. The phylograms of three genomic regions revealed correlation between geography and genetic structure. Furthermore, nucleotide diversity studies revealed a low divergence from the homologous sequences from GenBank and some variations within the groups were detected. The results presented in this study provide a better understanding of genetic variation and phylogenetic of GPGV isolates worldwide. © 2018, Deutsche Phytomedizinische Gesellschaft.