Yazar "Gokce, Zahide Neslihan Ozturk" seçeneğine göre listele
Listeleniyor 1 - 9 / 9
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Combined drought and heat stresses trigger different sets of miRNAs in contrasting potato cultivars(Springer Heidelberg, 2021) Gokce, Zahide Neslihan Ozturk; Aksoy, Emre; Bakhsh, Allah; Demirel, Ufuk; Caliskan, Sevgi; Caliskan, Mehmet EminMicroRNAs are small, non-coding RNAs that are responsible for regulation of gene expression during plant growth and development. Although there are many studies on miRNAs in other plants, little work has been done to understand the role of miRNAs in abiotic stress tolerance in potatoes. This study investigates changes in miRNA profiles of two different potato cultivars (tolerant, Unica and susceptible, Russet Burbank) in response to heat, drought and their combination. Transcriptomic studies revealed that miRNA profiles depend on the susceptibility and tolerance of the cultivar and also the stress conditions. Large number of miRNAs were expressed in Unica, whereas Russet Burbank indicated lesser number of changes in miRNA expression. Physiological and transcriptional results clearly supported that Unica cultivar is tolerant to combined drought and heat stress compared to Russet Burbank. Moreover, psRNATarget analysis predicted that major miRNAs identified were targeting genes playing important roles in response to drought and heat stress and their important roles in genetic and post-transcriptional regulation, root development, auxin responses and embryogenesis were also observed. This study focused on eight miRNAs (Novel_8, Novel_9, Novel_105, miR156d-3p, miR160a-5p, miR162a-3p, miR172b-3p and miR398a-5p) and their putative targets where results indicate that they may play a vital role at different post-transcriptional levels against drought and heat stresses. We suggest that miRNA overexpression in plants can lead to increased tolerance against abiotic stresses; furthermore, there should be more emphasis on the studies to investigate the role of miRNAs in combined abiotic stress in plants.Öğe Comparative transcriptomics of drought stress response of taproot meristem region of contrasting purple carrot breeding lines supported by physio-biochemical parameters(Springer Heidelberg, 2022) Gokce, Zahide Neslihan Ozturk; Gokce, Ali Fuat; Junaid, Muhammad Daniyal; Chaudhry, Usman KhalidCarrot is one of the nutritious vegetable crops sensitive to drought stress resulting in loss of quality and yield. There are a lot of studies on detailed molecular mechanisms of drought stress response of main crops; however, very little information available on vegetables, including carrots. Hence, in this study, we investigated root transcriptome profiles from the meristematic region of two contrasting purple carrot (B7262A, drought tolerant; P1129, drought sensitive) lines under varying stress levels (85% and 70%) by using RNA-Seq technique. The morpho-physiological and biochemical response of B7262A line exhibited tolerance behavior to both DS (85% and 70%). RNA-Seq analysis revealed that 15,839 genes were expressed commonly in both carrot lines. The carrot line B7262A showed regulation of 514 genes in response to 85% DS, whereas P1129 showed differential regulation of 622 genes under 70% DS. The B7262A carrot line showed higher upregulation of transcripts that suggested its resilient behavior contrary to P1129 line. Furthermore, validation of transcript gene by qRT-PCR also confirmed the RNA-Seq analysis resulting in elevated expression levels of MYB48 transcription factor, MAPK mitogen-activated protein kinase ANP1, GER geraniol 8-hydroxylase, ABA ABA-induced in somatic embryo 3, FBOX putative F-box protein, FRO ferric reduction oxidase, and PDR probable disease resistance protein. Current study provided unprecedented insights of purple carrot lines that can be potentially exploited for the screening and development of resilient carrot.Öğe Drought and salt stress effects on biochemical changes and gene expression of photosystem II and catalase genes in selected onion cultivars(Springer, 2021) Chaudhry, Usman Khalid; Gokce, Zahide Neslihan Ozturk; Gokce, Ali FuatOnion is the main condiment vegetable crop that is grown and consumed due to its rich nutraceutical properties. Globally, drought and salt stress are the major hurdles for onion growth and pose greater threat to the future crop growth. Numerous studies reported the response of vegetable crops with the acclimatization to fluctuating environmental conditions but data for onion appears to be limited on biochemical and gene expression changes. In this study, we investigated the response of seven onion cultivars from Turkey for biochemical and gene expression responses to drought and salt stresses under greenhouse conditions. There is limited data available on NCBI for onion genes; therefore, degenerate PCR approach was used to amplify the catalase (CAT) and photosystem (PSII) genes of onion to observe correlation between their activity with transcript levels. Results of biochemical attributes indicated that the cultivars Perama, Inci, and Seyhan performed better compared to other cultivars with the exposure to salt and drought stress. Additionally, these cultivars also accumulated higher transcript level of CAT gene and showed least decrease in the degradation of PSII mRNA. Overall, results revealed that the cultivars Perama, Inci, and Seyhan were tolerant, whereas the cultivars Elit and Hazar were grouped as sensitive to salt and drought stress conditions. Results of this study can be used for screening of tolerant and susceptible cultivars. Moreover, the tolerant cultivars Perama, Inci, and Seyhan can be used for future abiotic stress breeding programs.Öğe Effects of Salinity and Drought Stresses on the Physio-Morphological Attributes of Onion Cultivars at Bulbification Stage(Friends Science Publ, 2020) Chaudhry, Usman Khalid; Gokce, Zahide Neslihan Ozturk; Gokce, Ali FuatOnion (Allium cepa L.) has huge importance due to its health benefits. Salinity and drought stress appear to be the major threats towards the productivity of crops and vegetables across the globe. Although in literature several studies summarize responses of agricultural crops to abiotic stresses but data for onion appears to be limited. In this greenhouse study, seven onion cultivars were compared for their morphological and physiological responses to salinity stress (SS) and drought stress (DS). Salinity stress was applied to SS group through irrigating with water containing increasing doses of NaCl (100, 125, 150, 175 and 200 mM) in 3-day intervals, whereas water was suspended for 20 days to DS group for drought stress application. Salinity and drought stress decreased photosynthetic rate, lower leaf number, leaf length and bulb yield. SS interfered with root length and diameter, whereas thicker and elongated root length was noticed in response to DS. The cultivars 'Elit', 'Hazar' and 'Sampiyon' exhibited the reduction in photosynthetic rate. Maximum damage to chlorophyll contents was observed in cultivars 'Elit', 'Hazar' and 'Sampiyon' under SS and DS conditions. The cultivar 'Perama' manifested the highest bulb weight under SS and DS condition. The cultivars 'Perama' and 'Seyhan' were referred to as tolerant based on their overall performance under SS and DS, supported by the results of principal component analysis (PCA), however, the cultivars 'Elit', 'Hazar', and 'Sampiyon' performed poorly, so grouped as sensitive cultivars. Results of this study can be helpful in screening of tolerant and susceptible onion cultivars which will be useful for future breeding programs. (C) 2020 Friends Science PublishersÖğe Evaluation of miRNA Mediated Networking and Feedback Against Drought, Heat and Combined Stress Tolerance in Potato (Solanum tuberosum L.)(Springer, 2020) Asim, Arslan; Demirel, Ufuk; Bakhsh, Allah; Gokce, Zahide Neslihan Ozturk[Abstract Not Available]Öğe Individual and combined effect of drought and heat stresses in contrasting potato cultivars overexpressing miR172b-3p(Tubitak Scientific & Technological Research Council Turkey, 2021) Asim, Arslan; Gokce, Zahide Neslihan Ozturk; Bakhsh, Allah; Cayli, Ilknur Tindas; Aksoy, Emre; Csliskan, Sevgi; Caliskan, Mehmet EminMicroRNAs (miRNAs) are essential players of plant defence systems because of their involvement in reprogramming gene expression under adverse environmental conditions including drought and heat, which are considered major players in limiting crop productivity. miR172b-3p was previously determined as a remarkable stress-responsive miRNA in our next-generation sequencing (NGS) analysis in potato. This study aims to understand the functions of miR172b-3p and its target (ERTF RAP2-7-like) under drought, heat, and combined treatments by overexpressing the miR172b-3p in stress-tolerant (Unica) and sensitive (Russet Burbank) potato cultivars. miR172b-3p overexpression in transgenic lines suppressed the ERTF RAP2-7-like expression leading to enhanced carbon fixation efficiency. Meanwhile, the accumulation of hydrogen peroxide (H2O2) was reduced in both cultivars, proving that it is involved in the front-line tolerance mechanism against individual drought, heat, and their combination. In conclusion, our results prove that the stress tolerance could be enhanced by miR172b-3p-mediated negative regulation of ERTF RAP2-7-like gene in potato under drought, heat, and their combination. Our findings represent the first step towards the improvement of tolerance against multiple abiotic stresses in potato.Öğe Investigation of drought induced biochemical and gene expression changes in carrot cultivars(Springer, 2023) Junaid, Muhammad Daniyal; Gokce, Zahide Neslihan Ozturk; Gokce, Ali FuatBackground Carrot is the most important vegetable in Apiaceae family, and it is consumed globally due to its high nutritional quality. Drought stress is major environmental constraint for vegetables especially carrot. Limited data is available regarding the mechanisms conferring drought tolerance in carrot. Methods and Results Eight commercial carrot cultivars were used in this study and subjected to drought stress under semi-controlled greenhouse conditions. Biochemical, antioxidant enzymatic activity and changes in transcript level of drought related genes was estimated, the gene expression analysis was done by using qRT-PCR in comparison with reference gene expression Actin (Act1). Results revealed that cultivars Coral Orange, Tendersweet and Solar Yellow were tolerant to drought stress, which was supported by their higher transcript levels of catalase gene (CAT), superoxide dismutase genes (Cu/ZN-SOD, Cu/Zn-SDC) in these cultivars. The downregulation of PDH1 gene (Proline dehydrogenase 1) was also observed that was associated with upregulation of proline accumulation in carrot plants. Moreover, results also suggested that PRT genes (Proline transporter genes) played a key role in drought tolerance in carrot cultivars. Conclusion Among the cultivars studied, Coral Orange showed overall tolerance to drought stress conditions, whereas cultivars Cosmic Purple and Eregli Black were sensitive based on their biochemical and gene expression levels. According to our knowledge, this is the first comparative study on drought tolerance in several carrot cultivars. It will provide a background for carrot breeding to understand biochemical and molecular responses of carrot plant to drought stress and mechanisms behind it.Öğe Morphological, physiological, and biochemical responses of onion (Allium cepa L.) breeding lines to single and combined salt and drought stresses(Springer, 2022) Gokce, Zahide Neslihan Ozturk; Gokce, Ali Fuat; Junaid, Muhammad Daniyal; Chaudhry, Usman KhalidAbiotic stresses deteriorate plant growth resulting in devastating yield losses. Salt stress solely cause ionic toxicity and disturbed homeostasis, whereas combined salt and drought stress has more pronounced effects on plants. This study aimed to screen 32 Turkish onion breeding lines and commercial cultivars based on their morpho-physiological and biochemical responses after exposure to drought, salt, and salt + drought stresses at the bulbification stage under greenhouse conditions. Physiological characteristics, such as gaseous exchange traits, chlorophyll index, leaf temperature, and morphological traits including the number of leaves, length, and diameter of leaf were measured during stress treatment, and yield response of the onions was quantified by measuring bulb length, bulb diameter, and bulb weight after harvest. Proline and malondialdehyde accumulation was estimated for the biochemical effect of stress on onion genotypes. All genotypes responded differentially to the applied single and combined stresses. Overall results revealed that in the breeding lines K25, U6, U17 and commercial cultivar K58, the bulb weight (41.71-47.93 g) was significantly (p <= 0.05) lower, therefore they were grouped as sensitive across all stresses; whereas in the breeding lines K41, U47, U49 and commercial cultivar K52, the bulb weight (96.75-106.31 g) was significantly (p <= 0.05) higher among all the tested breeding lines and commercial cultivars and therefore found to be the most stable upon stress. These resilient genotypes can be used as breeding material for future abiotic stress studies.Öğe Response of Contrasting Potato Cultivars to Single and Combined Abiotic Stresses Harboring miR156d-3p(Springer, 2023) Asim, Arslan; Demirel, Ufuk; Bakhsh, Allah; Gokce, Zahide Neslihan Ozturk[Abstract Not Available]
