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Öğe Characterization of spider silks weaved by different species living in the Black sea region of Turkey(Int Union Crystallography, 2008) Batat, Pinar; Ide, Semra; Turkes, Tuncay; Mengen, Orhan; Ozcan, Yusuf; Celik, Omer; Kriechbaum, Manfred[Abstract Not Available]Öğe In vitro and in vivo investigation of Argiope bruennichi spider silk-based novel biomaterial for medical use(Wiley, 2024) Turan, Secil Karahisar; Suloglu, Aysun Kilic; Ide, Semra; Turkes, Tuncay; Barlas, NurhayatAs a natural and biocompatible material with high strength and flexibility, spider silk is frequently used in biomedical studies. In this study, the availability of Argiope bruennichi spider silk as a surgical suture material was investigated. The effects of spider silk-based and commercial sutures, with and without Aloe vera coating, on wound healing were evaluated by a rat dorsal skin flap model, postoperatively (7th and 14th days). Biochemical, hematological, histological, immunohistochemical, small angle x-ray scattering (SAXS) analyses and mechanical tests were performed. A. bruennichi silk did not show any cytotoxic effect on the L929 cell line according to MTT and LDH assays, in vitro. The silk materials did not cause any allergic reaction, infection, or systemic effect in rats according to hematological and biochemical analyses. A. bruennichi spider silk group showed a similar healing response to commercial sutures. SAXS analysis showed that the 14th-day applications of A. bruennichi spider silk and A. vera coated commercial suture groups have comparable structural results with control group. In conclusion, A. bruennichi spider silk is biocompatible in line with the parameters examined and shows a healing response similar to the commercial sutures commonly used in the skin.Öğe Nano scale structural analyses on Turkey/Taiwan originated spiders' cocoons(Int Union Crystallography, 2017) Ide, Semra; Orujalipoor, Ilghar; Chuang, Wei-Tsung; Jeng, U-Ser; Ozcan, Yusuf; Turkes, Tuncay[Abstract Not Available]Öğe Nuclear Magnetic Resonance (NMR) study of Palm Kernel Stearin: Effects of cooling rate on crystallization behaviour(Elsevier, 2022) Okur, Ilhami; Ozel, Baris; Ucbas, Derya; Grunin, Leonid; Okur, Purlen Sezer; Alpas, Hami; Ide, SemraIn this study, effects of different cooling rates (0.5,3.3,4.7 and 6.9 degrees C/min) on the crystallization behavior of palm-kernel-stearin (PKS) were studied by low-field NMR relaxometry. According to results, solid fat content (SFC), longitudinal relaxation time (T1), second moment (M2) and degree of crystallinity (%) of the samples increased with increase in cooling rate from 0.5 to 6.9 degrees C/min. In contrast, transverse relaxation time (T2) demonstrated an opposite behavior with respect to T1 and decreased when the cooling rate increased. Additionally, effects of cooling rate on the changes of polymorph structures were detected by X-ray measurements. Degree of crystallinity showed high Pearson correlation values (alpha <= 0.05) with SFC (r = 0.771) and T1(r = 0.932). Changes in the crystal polymorphs could also be explained by NMR parameters to some extent as can be observed by the strong correlation between the 13 crystal content and T2 (r = 0.927). At the highest cooling rate, 13 ' crystals were the dominant polymorphic form and constituted 75(%) of the total crystals present. Results of this study suggested that NMR relaxometry could be used as a complementary tool to interpret the crystallization behavior of PKS.Öğe Structural characterization of a variety of spider silks from Turkey using different biophysical techniques(IOS PRESS, 2011) Ide, Semra; Bayari, Sevgi Haman; Turkes, Tuncay; Mergen, Y. Orhan; Celik, Omer; Butun, Vural; Kriechbaum, ManfredVarious experimental methods (XRD, FTIR, SAXS, DLS and SEM) were used to investigate structures of the silk samples weaved by spiders in Turkey. Silk samples were collected from the natural habitats belonging to the spider species (i.e., Near Salt Lake/Sereflikochisar,. Ivriz Dam/Eregli-Konya, Karatas Lake/Burdur and several locations in Black Sea Region of Turkey). Among all collected species, Araneidae (Araneus angulatus, Argiope bruennichi, Argiope lobata, Larinoides cornutus), Eresidae (Eresus cinnaberinus), Agelenidae (Agelena labyrinthica), Oecobidae (Uroctea durandi), Miturgidae (Cheiracanthium erraticum), Salticidae (Heliophanus flavipes, Philaeus chrysops), Theridiidae (Steatoda paykulliana) and Gnaphosidae were found significant amongst other spider species. Structural differences and similarities of these species were reported. Diameter range of draglines, inter crystallite distance of nano-size structures, mean size range of nano-structure aggregations, crystallite size range, information about protein content were obtained. The mechanical properties of some silk fibers were also investigated.Öğe X-ray Scattering and Diffraction Studies on Spider Silk Cocoons(Int Union Crystallography, 2009) Soylu, Elif Hilal; Ide, Semra; Turkes, Tuncay; Kocatepe, Neslihan; Mergen, Orhan; Celik, Omer[Abstract Not Available]
