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    CRISPR/Cas9: A New Genome Editing Tool to Accelerate Cotton (Gossypium spp.) Breeding
    (Springer International Publishing Ag, 2019) Sattar, Muhammad N.; Iqbal, Zafar; Das Dangol, Sarbesh; Bakhsh, Allah
    Cotton has a tremendous economic value worldwide due to its high-quality fiber, edible oil and protein contents. However, the intensifying scenario of human population expansion and global environmental changes demand a proportionate increase in cotton production. In the past, several successful attempts have been made by introgression of many quality- and yield-related traits into elite cotton cultivars through conventional breeding. However, those measures are time consuming due to the reliance on introgression of naturally-existing genetic variation through extensive backcrossing. Nonetheless, plant breeding can be accelerated through modern genome editing (GE) tools. Various GE techniques including zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced palindromic repeats and CRISPR-associated proteins systems (CRISPR/Cas)-based approaches have been successfully employed for various crop plants. Among them, CRISPR/Cas-based approaches hold great GE potential due to their simplicity, competency and versatility. In cotton, this system can regulate the gene expression associated with quality traits, to circumscribe phytopathogens and/or to stack molecular traits at a desired locus. In gene stacking through site-specific endonucleases, the desired genes can be introduced in close proximity to a specific locus in the cotton genome with a low risk of segregation. However, such executions are tedious to achieve through classical breeding techniques. Moreover, through the CRISPR/Cas-based approaches, transgene-free cotton plants can easily be produced by selfing or backcrossing to meet the current genetically modified organisms (GMO) guidelines. In this chapter, we address the potential application of CRISPR/Cas-based approaches in available whole cotton genomes to sustain cotton productivity, and achieve genetic improvement, pathogen resistance and agronomic traits. Future prospects of GE applications in cotton breeding are also addressed.
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    Diversity and Management of Plant Viruses Infecting Rice
    (wiley, 2022) Iqbal, Zafar; Sattar, Muhammad Naeem; Naqqash, Muhammad Nadir
    Rice (Oryza sativa L., family Poaceae) is the leading cereal crop that is widely cultivated across the globe. Asia is the largest producer of rice with over 7.05 billion tons production in 2018, followed by the United States (38 million tons [MT]), Africa (33 MT), Europe (4 MT), and Oceania (0.65 MT). While the leading rice-producing countries are China (148.5 million MT) followed by India (116.42 million MT), Indonesia (36.7 million MT), Bangladesh (34.91 MT), and Vietnam (27.77 million MT). Rice supplies 21% of energy and 15% of protein to humans and plays a crucial role in the global food chain. However, rice cultivation is under continuous stress due to several biotic and abiotic constraints. Among the biotic constraints, rice-infecting viruses (RIVs) and their insect vectors cause enormous yield losses to worldwide rice production. RIVs encompass huge genomic diversity and include single-stranded, double-stranded, negative-sense single-stranded, negative-sense double-stranded, positive-sense single-stranded, and ambisense viruses. More than 15 RIVs are known and 10 of these RIVs pose a significant threat to Asian rice production. To sustain the global food security, it is of dire need to curb the RIVs and their insect vectors simultaneously. Several conventional to modern approaches have been employed to sustain the rice production against RIVs. Nonetheless, the contemporary CRISPR-Cas-based approaches and its expanding toolkit can offer unlimited utilities to improve rice yield and control RIVs and their insect vectors via transgene-free genome-editing capabilities. The importance of rice, RIVs, and their control strategies are discussed. © The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2022.