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Öğe Detection of Theileria and Babesia species in sheep and goats by microscopy and molecular methods in Nigde province, Turkey(Ecole Nationale Veterinaire Toulouse, 2019) Karatepe, B.; Ozubek, S.; Karatepe, M.; Aktas, M.The present study was conducted to investigate presence and prevalence of Theileria and Babesia in sheep and goats of Nigde by microscopic and molecular methods. For this purpose, it was chosen a total of 690 small ruminants including 520 sheep and 170 goats between 1-10 year olds belonging to 28 flocks in Nigde province, in Central Anatolia of Turkey between April-July 2014. Blood smears were prepared from the blood samples, stained with Giemsa, and examined under the light microscope for Theileria and Babesia piroplasms. The piroplasm DNAs of Theileria and Babesia species was amplified using the RLB-F2 ve RLB-R2 primers. The polymerase chain reaction (PCR) products were hybridized to the membrane connected species-specific probes. The ticks were removed from sheep and goats and they were identified. Out of blood samples examined microscopically, 119 sheep (17.2%) were positive for Theileria spp. or Babesia spp., whereas no goats were positive. Of 690 animals 344 (49.8%) were positive for Theileria or Babesia by RLB method. Theileria ovis was the most common blood parasites in sheep and goats in the region with 46.9%, this is followed by B. ovis with 3.5% and Theileria sp. MK with 1.4%. The other Theileria species (T. lestoquardi, T. luwenshuni, T. uilenbergi) and genotypes (Theileria sp. OT1, Theileria sp. OT3) and Babesia species (B. motasi, B. crassa) were not detected in sheep and goats in the region. A total of 1361 ticks were collected in sheep and goats and they identified as Rhipicephalus bursa, R. turanicus, Hyalomma marginatum, Hy. excavatum, Hy. anatolicum, Haemophsalis parva, Hae. punctata, Hae. sulcata and Dermacentor marginatus. In determining blood parasites in sheep and goats, it was determined that PCR and RLB results were more susceptible than microscopy (P < 0.05,chi(2) = 105.823; P < 0.05,chi(2) = 164.548), and RLB than to PCR.Öğe Epidemiology of Oestrus ovis L. infestation in sheep in Nigde province, Turkey(ECOLE NATIONALE VETERINAIRE TOULOUSE, 2014) Karatepe, B.; Karatepe, M.; Guler, S.The objective of this study is to examine the prevalence of oestrosis caused by the Oestrus ovis L. in the sheep of Nigde province, Turkey. The study examines the presence of O. ovis larvae in 364 sheep heads collected from Nigde slaughterhouses between August 2010 and July 2011 with an average of about 30 per month. Oestrus ovis larvae were detected in the 82 (22.52%) out of the 364 sheep heads tested. Prevalence of O. ovis in the sheep was identified with regards to the months and seasons 0-60% and 11.11-49.47%, respectively. In addition, the infestation rate in the 6-9 months old sheep was found to be 23.28%, and 21.71% in the 1-2 years old sheep. While differences in the rates of infestation in terms of months and seasons were determined to be statistically significant (p<0.01), in terms of age groups it was found statistically insignificant (p>0.05). A total of 474 larvae were encountered in the infested sheep, 49 (10.33%) of those larvae were identified as L-1, 236 (49.78%) were identified as L-2, and 189 (39.87%) were identified as L-3.Öğe Prevalence of anti-neospora caninum antibodies in sheep in Nevşehir province, Turkey(Israel Veterinary Medical Association, 2020) Karatepe, M.; Karatepe, B.This study was carried out in order to investigate the presence of anti-Neospora caninum antibodies in sheep in the Nevşehir province in Turkey. Blood samples were taken from randomly selected 180 sheep, which were between 1 and 7 years of age and from different locations in Nevşehir province. All of the serum samples were ELISA tested for N. caninum antibodies. Fourteen (7.8%) of serum samples were found to be seropositive for N. caninum antibodies by using ELISA. According to the age, antibodies to N. caninum were found in six (7.7%) of 78 sheep at the age of 1-2 years of age, in four (7.3%) of 55 sheep at the age of 3-4 years of age, and in four (8.5%) of 47 sheep at the age of 5-7 years of age. Anti-N. caninum antibodies were detected in 13 (8.0%) of 162 ewes and 1 (5.6%) of 18 rams. Moreover, 34.8%, the highest seropositivity rate in this study was found in the central regions of Nevşehir, while the no antibodies were detected in Kozaklı, Acıgöl, Hacıbektaş and Derinkuyu regions. The seropositivity rates of N. caninum were not statistically significant with regard to age groups (P=0.342), gender (P=0.261) and in relation to the study centers (P=0.173). This is the first serologic survey for N. caninum antibodies performed on sheep in Nevşehir province. © 2020, Israel Veterinary Medical Association. All rights reserved.Öğe Prevalence of Anti-Neospora caninum Antibodies in Sheep in Nevsehir Province, Turkey(Israel Veterinary Medical Assoc, 2020) Karatepe, M.; Karatepe, B.This study was carried out in order to investigate the presence of anti-Neospora caninum antibodies in sheep in the Nevsehir province in Turkey. Blood samples were taken from randomly selected 180 sheep, which were between 1 and 7 years of age and from different locations in Nevsehir province. All of the serum samples were ELISA tested for N. caninum antibodies. Fourteen (7.8%) of serum samples were found to be seropositive for N. caninum antibodies by using ELISA. According to the age, antibodies to N. caninum were found in six (7.7%) of 78 sheep at the age of 1-2 years of age, in four (7.3%) of 55 sheep at the age of 3-4 years of age, and in four (8.5%) of 47 sheep at the age of 5-7 years of age. Anti-N. caninum antibodies were detected in 13 (8.0%) of 162 ewes and 1 (5.6%) of 18 rams. Moreover, 34.8%, the highest seropositivity rate in this study was found in the central regions of Nevsehir, while the no antibodies were detected in Kozakli, Acigol, Hacibektas and Derinkuyu regions. The seropositivity rates of N. caninum were not statistically significant with regard to age groups (P=0.342), gender (P=0.261) and in relation to the study centers (P=0.173). This is the first serologic survey for N. caninum antibodies performed on sheep in Nevsehir province.Öğe Seroprevalence of Hypodermosis in Cattle in Nigde Province of Turkey by Comparison of Commercial and Indirect-ELISA Methods(ISRAEL VETERINARY MEDICAL ASSOC, 2013) Karatepe, M.; Simsek, S.; Karatepe, B.; Cayvaz, M.; Sevgili, M.; Balkaya, I.The aim of this study was to determine the seroprevalence of hypodermosis in cattle of Nigde province, located in the centre of Turkey. A total of 183 serum samples were collected between December 2009 and February 2010 for this goal. The serum samples were analyzed with a commercial ELISA kit (Pourquier ELISA Hypodermosis bovine serum screening) and an indirect-ELISA (using antigen Hypodermin C) methods. The results of the tests with the commercial ELISA kit showed that 53 (28.9%) out of the 183 cattle were seropositive for hypodermosis. Eleven (18.3%) out of the 60 female and 42 (34.1%) out of the 123 male, were found to be seropositive. The seropositivity rate was calculated as 21.8% (40/183) by indirect-ELISA, and the seroprevalence was 18.3% (11/60) and 23.5% (29/123) in female and males, respectively. A statistically significant correlation was observed when the two test methods for detecting seropositivity of Hypoderma were compared (p<0.001). This study is to the best of our knowledge the first serological survey on hypodermosis in cattle from the Nigde province of Turkey.Öğe Seroprevalence of Neospora caninum in Cattle in Nigde Province, Turkey(ISRAEL VETERINARY MEDICAL ASSOC, 2016) Karatepe, B.; Karatepe, M.This study was carried out in order to investigate the presence of Neospora caninum antibodies in local cattle breeds in the Nigde province. The animals used in this study consisted of cattle of at least one year of age slaughtered at a local abattoir in Nigde province of Turkey. Blood samples were obtained from 264 cattle (207 male, 57 female) and serum samples were tested for antibodies to N. caninum using commercial competitive ELISA (c-ELISA) (VMRD, Pulman, WA, USA). According to the serological examination, 70 (26.51%) of 264 cattle were seropositive for N. caninum. Anti-N. caninum antibodies were detected in 63 (30.43%) of 207 male cattle and 7 (12.28%) of 57 female cattle. There was a statistically significant differences in seropositivity between genders (p < 0.05). This is the first serologic survey for N. caninum antibodies performed on cattle in Nigde province.Öğe Seroprevalence of toxoplasmosis in horses in Nigde Province of Turkey(SPRINGER, 2010) Karatepe, B.; Babur, C.; Karatepe, M.; Kilic, S.The present study was conducted to investigate the prevalence of Toxoplasma gondii specific antibodies in local horses from four districts of Nigde in the middle of Turkey, between April-June 2004. Serum samples were obtained a total of 125 horses which consisted of 81 (50 female, 31 male) 1-10 years old and 44 (25 female, 19 male) 11-20 years old and tested for antibodies to T. gondii using the Sabin Feldman Dye Test (SFDT). According to the results of the SFDT, antibodies to T. gondii were found by the SFDT in 9 (7.2%) of 125 sera with the titers of 1:16 (8 horses) and 1:64 (1 horse). Antibodies to T. gondii were found in 6 (7.40%) of 81 horses (1-10 years old) and 3 (6.81%) of 44 horses (11-20 years old). From the 5 (10%) out of 50 male horses and the 4 (5.33%) out of 75 female horses were detected anti-T. gondii antibodies. No statistically significant difference in age groups (p > 0.01) and genders (p > 0.005) were observed between the seropositive and seronegative horses using the x(2) test. Seropositivity rates ranged from 2.85% to 11.42%, depending on the study sites. In regard to study sites, there was no statistically significant difference was found (p > 0.005). This is the first serological report on toxoplasmosis in horses from Nigde of Turkey.
