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Öğe A note on seroprevalence of toxoplasma gondii in Turkeys(2008) Babür C.; Karatepe B.; Karatepe S.K.M.; Ergün G.[No abstract available]Öğe Eimeria species (Apicomplexa: Eimeriidae) detected from the Anatolian ground squirrel, Spermophilus xanthophrymnus (Rodentia: Sciuridae) in Nigde province, Turkey [Nigde yöresinde Anadolu tarla sincabi{dotless}, Spermophilus xanthophrymnus (Rodentia: Sciuridae)'da bulunan Eimeria Türleri](2010) Çiçek H.; Karatepe M.; Karatepe B.; Cakir M.; Eser M.Intestinal contents of 105 Anatolian ground squirrels (Spermophilus xanthophrymnus) caught from Nigde province were examined to determine the types and prevalence of eimerian species. Eimerian oocysts were found in 27.6% of all the samples examined by sucrose-flotation. Four species were recovered from Anatolian ground squirrels, including Eimeria callospermophili (prevalence: 25.7%), E. morainensis (2.8%), E. pseudospermophili (1.9%), and E. lateralis (0.9%).Öğe Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods(Nature Publishing Group, 2018) Karatepe B.; Aksoy S.; Karatepe M.The aim of this study was to determine the presence of Wolbachia spp. and Spiroplasma spp. in natural populations of sand flies in Turkey by molecular methods. A total of 40 Phlebotomus specimens (19 female and 21 male) were used in this study. Genomic DNA from whole sand flies was isolated and Wolbachia spp. infection prevalence was investigated by using Wolbachia gene specific primer sets (wsp and GroEL). In addition, the DNA were analyzed for the presence of Spiroplasma infections utilizing bacterium specific 16 S rDNA PCR-amplification primers. Results of this analysis showed a Wolbachia infection prevalence of 70% (28/40). There was no sex-bias in infection prevalence, being 76% (16/21) and 63% (12/19) in males and females, respectively. Analysis of Spiroplasma infections indicated that 26% (5/19) of female sand flies were positive for infection, while none of the screened males (0/21) were positive. Of the 40 sand fly samples, only 2 were found to be positive for both Wolbachia spp. and Spiroplasma spp. The present study demonstrates the presence of Wolbachia and Spiroplasma infections in the natural sand fly populations in Turkey. This is the first report on Spiroplasma infection in the sand flies from Turkey. © 2018 The Author(s).Öğe Prevalence of Toxoplasma gondii antibodies in domestic (Columba livia domestica) and wild (Columba livia livia) pigeons in Nigde region, Turkey.(2011) Karatepe M.; Kiliç S.; Karatepe B.; Babür C.The present study was conducted to investigate the prevalence of Toxoplasma gondii specific antibodies in domestic (Columba livia domestica) and wild (Columba livia livia) pigeons between October 2003-June 2004. Blood samples were collected from 216 pigeons, consisting of 105 (55 female, 50 male) domestic pigeons and 111 (53 female, 58 male) wild pigeons. The sera were tested for T. gondii antibodies using the Sabin Feldman Dye Test (SFDT). One of the 105 (0.95%) domestic pigeon and one of the 111 (0.90%) wild pigeon were found to be seropositive for T. gondii antibodies at the titer of 1:16. This is the first serological study on toxoplasmosis in the domestic and wild pigeon in the Nigde region of Turkey.Öğe Seroprevalence of hypodermosis in cattle in Nigde Province of Turkey by comparison of commercial and indirect-ELISA method(2013) Karatepe M.; Simsek S.; Karatepe B.; Cayvaz M.; Sevgili M.; Balkaya I.The aim of this study was to determine the seroprevalence of hypodermosis in cattle of Nigde province, located in the centre of Turkey. A total of 183 serum samples were collected between December 2009 and February 2010 for this goal. The serum samples were analyzed with a commercial ELISA kit (Pourquier ELISA Hypodermosis bovine serum screening) and an indirect-ELISA (using antigen Hypodermin C) methods. The results of the tests with the commercial ELISA kit showed that 53 (28.9%) out of the 183 cattle were seropositive for hypodermosis. Eleven (18.3%) out of the 60 female and 42 (34.1%) out of the 123 male, were found to be seropositive. The seropositivity rate was calculated as 21.8% (40/183) by indirect- ELISA, and the seroprevalence was 18.3% (11/60) and 23.5% (29/123) in female and males, respectively. A statistically significant correlation was observed when the two test methods for detecting seropositivity of Hypoderma were compared (p<0.001). This study is to the best of our knowledge the first serological survey on hypodermosis in cattle from the Nigde province of Turkey.Öğe Seroprevalence of Toxoplasma gondii in Sheep from Nevşehir Province inTurkey(2017) Özmutlu Çakmak D.; Karatepe B.OBJECTIVE: The goal of this study was to investigate the seroprevalence of Toxoplasma gondii (T. gondii) in sheep from Nevşehir Province in Turkey.METHODS: Blood samples were taken from 180 sheep aged between 1 and 7 years, which were randomly selected from seven different study sites in Nevşehir Province. The enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies to T. gondii was performed on all the serum samples.RESULTS: Eighteen (10%) serum samples were found to be seropositive for T. gondii antibodies. The highest seropositivity rate (11.53%) was found in sheep aged between 1 and 2 years, whereas the lowest seropositivity rate (8.51%) was found in sheep aged between 5 and 7 years old. Eighteen of the 162 ewes (11.1%) were found to be seropositive, whereas none of the 18 tested rams were seropositive. In addition, considering the study site location, the highest seropositivity rate was in Avanos (32%), whereas the lowest seropositivity rate was in Kozakli (2.9%). The T. gondii seropositivity rates were statistically insignificant with regard to age groups and gender (p>0.05), whereas they were statistically significant (p<0.05) with regard to study centers.CONCLUSION: This is the first serological report on toxoplasmosis in sheep from Nevşehir Province in Turkey.
