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Öğe Blood parasites detected from Anatolian squirrel, Spermophilus xanthophrymnus (Rodentia: Sciuridae) in Nigde Province, Turkey [Nigde yöresinde anadolu tarla sincabi, Spermophilus xanthophrymnus (Rodentia: Sciuridae)'da bulunan kan parazitleri](2009) Çiçek H.; Karatepe M.; Çakir M.; Eser M.The study was conducted on 105 Anatolian squirrel, Spermophilus xanthophrymnus (Rodentia: Sciuridae) in Nigde which is province of Turkey from April 2003 to August 2003. Trypanosoma lewisi and Babesia microti were detected in the blood smears of 5 and 2 squirrels, respectively. This study reports the presence of T. lewisi and B. microti in Anatolian squirrel in Turkey for the first time.Öğe Eimeria species (Apicomplexa: Eimeriidae) detected from the Anatolian ground squirrel, Spermophilus xanthophrymnus (Rodentia: Sciuridae) in Nigde province, Turkey [Nigde yöresinde Anadolu tarla sincabi{dotless}, Spermophilus xanthophrymnus (Rodentia: Sciuridae)'da bulunan Eimeria Türleri](2010) Çiçek H.; Karatepe M.; Karatepe B.; Cakir M.; Eser M.Intestinal contents of 105 Anatolian ground squirrels (Spermophilus xanthophrymnus) caught from Nigde province were examined to determine the types and prevalence of eimerian species. Eimerian oocysts were found in 27.6% of all the samples examined by sucrose-flotation. Four species were recovered from Anatolian ground squirrels, including Eimeria callospermophili (prevalence: 25.7%), E. morainensis (2.8%), E. pseudospermophili (1.9%), and E. lateralis (0.9%).Öğe Investigation of Wolbachia spp. and Spiroplasma spp. in Phlebotomus species by molecular methods(Nature Publishing Group, 2018) Karatepe B.; Aksoy S.; Karatepe M.The aim of this study was to determine the presence of Wolbachia spp. and Spiroplasma spp. in natural populations of sand flies in Turkey by molecular methods. A total of 40 Phlebotomus specimens (19 female and 21 male) were used in this study. Genomic DNA from whole sand flies was isolated and Wolbachia spp. infection prevalence was investigated by using Wolbachia gene specific primer sets (wsp and GroEL). In addition, the DNA were analyzed for the presence of Spiroplasma infections utilizing bacterium specific 16 S rDNA PCR-amplification primers. Results of this analysis showed a Wolbachia infection prevalence of 70% (28/40). There was no sex-bias in infection prevalence, being 76% (16/21) and 63% (12/19) in males and females, respectively. Analysis of Spiroplasma infections indicated that 26% (5/19) of female sand flies were positive for infection, while none of the screened males (0/21) were positive. Of the 40 sand fly samples, only 2 were found to be positive for both Wolbachia spp. and Spiroplasma spp. The present study demonstrates the presence of Wolbachia and Spiroplasma infections in the natural sand fly populations in Turkey. This is the first report on Spiroplasma infection in the sand flies from Turkey. © 2018 The Author(s).Öğe Prevalence of Toxoplasma gondii antibodies in domestic (Columba livia domestica) and wild (Columba livia livia) pigeons in Nigde region, Turkey.(2011) Karatepe M.; Kiliç S.; Karatepe B.; Babür C.The present study was conducted to investigate the prevalence of Toxoplasma gondii specific antibodies in domestic (Columba livia domestica) and wild (Columba livia livia) pigeons between October 2003-June 2004. Blood samples were collected from 216 pigeons, consisting of 105 (55 female, 50 male) domestic pigeons and 111 (53 female, 58 male) wild pigeons. The sera were tested for T. gondii antibodies using the Sabin Feldman Dye Test (SFDT). One of the 105 (0.95%) domestic pigeon and one of the 111 (0.90%) wild pigeon were found to be seropositive for T. gondii antibodies at the titer of 1:16. This is the first serological study on toxoplasmosis in the domestic and wild pigeon in the Nigde region of Turkey.Öğe Seroprevalence of hypodermosis in cattle in Nigde Province of Turkey by comparison of commercial and indirect-ELISA method(2013) Karatepe M.; Simsek S.; Karatepe B.; Cayvaz M.; Sevgili M.; Balkaya I.The aim of this study was to determine the seroprevalence of hypodermosis in cattle of Nigde province, located in the centre of Turkey. A total of 183 serum samples were collected between December 2009 and February 2010 for this goal. The serum samples were analyzed with a commercial ELISA kit (Pourquier ELISA Hypodermosis bovine serum screening) and an indirect-ELISA (using antigen Hypodermin C) methods. The results of the tests with the commercial ELISA kit showed that 53 (28.9%) out of the 183 cattle were seropositive for hypodermosis. Eleven (18.3%) out of the 60 female and 42 (34.1%) out of the 123 male, were found to be seropositive. The seropositivity rate was calculated as 21.8% (40/183) by indirect- ELISA, and the seroprevalence was 18.3% (11/60) and 23.5% (29/123) in female and males, respectively. A statistically significant correlation was observed when the two test methods for detecting seropositivity of Hypoderma were compared (p<0.001). This study is to the best of our knowledge the first serological survey on hypodermosis in cattle from the Nigde province of Turkey.Öğe Seroprevalence of neospora caninum in goats in nigde province [Nigde yöresi keçilerinde neospora caninum'un seroprevalansi{dotless}](2011) Cayvaz M.; Karatepe M.This study was performed to investigate seroprevalence of Neospora caninum in goats in Nigde province between February 2010-May 2010. A total of 181 goats which were one year old or more than one year old, were selected from eight diferent study sites (Bahçeli, Centrum-Tepeköy, Bor, Çamardi{dotless} Celaller Village, Ki{dotless}zi{dotless}lca, Çukurkuyu, Uluki{dotless}şla, ve Central Çamardi{dotless}) as the study sample and N. caninum antibodies were tested in these goats serum using ELISA. The 47 out of 181 sera (25.9%) were found to be seropositive for N. caninum. In the serologic examinations, the seropositivity rate was highest in 5 years old (42.3%) while it was lowest in 3 years old (13.8%) in goats. The 6 (37.5%) out of 16 aborted goats and 41 (24.8%) out of 165 non-aborted were detected as seropositive. The highest seropositivity rate of study sites was determined in Çamardi{dotless} as 54.5% in addition to this, none of examined goats in Bahçeli and Ki{dotless}zi{dotless}lca detected seropositivity. The diference between the seropositivity rates to N.caninum among age groups and study sites was statistically significant (P<0.05, P<0.01, respectively) while the diference to N. caninum among aborted and non-aborted goats was statistically insignificant (P>0.05). As a result, this is the first serologic survey for N. caninum performed on goats in Nigde province and neosporosis should be taken into consideration in abortion cases.