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    Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney
    (Cukurova Univ, Fac Medicine, 2022) Gunturk, Inayet; Seydel, G. Seyda; Dagli, Fatma; Yay, Arzu; Yazici, Cevat; Kose, Kader
    Purpose: Cisplatin is one of the most potent and widely used chemotherapeutic agents for the treatment of a wide variety of solid organ cancers. However, due to various side-effects such as nephrotoxicity, its therapeutic applications are limited. In the current study, it was aimed to investigate the effects of N-acetylcysteine (NAC), which is an effective antioxidant and anti-inflammatory agent, on cisplatin-induced apoptosis in rat kidneys. Materials and Methods: Twentyfour male Wistar rats were separated into 4 equal groups: Control, NAC-250, cisplatin (CP), and CP+NAC groups. Rats in the experimental groups were treated with intraperitoneally (i.p.) single-dose cisplatin (10 mg/kg) and NAC (i.p., 250 mg/kg) for 3 days. Results: At the end of the experiment, nephrotoxicity was confirmed by blood urea nitrogen and creatinine levels, and the apoptotic changes were demonstrated by TdT-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) and caspase-3 levels in rat kidneys. The number of TUNEL-positive cells and caspase-3 levels were significantly increased by cisplatin. Treating the rats with NAC significantly decreased TUNEL-positive cells and caspase-3 levels. Conclusion: These data suggest that apoptotic cell death is involved in the pathogenesis of cisplatin-induced nephrotoxicity, and that the inhibition of apoptosis plays a central role in the beneficial effects of NAC.
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    The effect of N-acetylcysteine on inflammation and oxidative stress in cisplatin-induced nephrotoxicity: a rat model
    (Tubitak Scientific & Technological Research Council Turkey, 2019) Gunturk, Inayet; Yazici, Cevat; Kose, Kader; Dagli, Fatma; Yucel, Bilal; Yay, Arzu
    Background/Aim: Cisplatin is a highly effective chemotherapeutic agent used in the treatment of solid organ cancers. Besides its chemotherapeutic effectiveness, cisplatin administration is associated with numerous side effects. Of those, the most clinically significant and common effect is nephrotoxicity. Recent studies reported that oxidative stress and inflammation are probably the most important mechanisms that contribute to the nephrotoxicity. N-acetylcysteine (NAC) is an antioxidant and antiinflammatory agent. In the present study, the effects of NAC on cisplatin-induced nephrotoxicity were investigated. Materials and methods: Rats were divided into four groups each including eight rats: CONT, NAC-250, CP, and CP+NAC. Rats in experimental groups were treated intraperitoneally (i.p.) with a single dose of cisplatin (10 mg/kg body weight) and i.p. with NAC (250 mg/kg body weight) for three consecutive days. Nephrotoxicity was determined by plasma BUN and creatinine levels. In tissue samples, myeloperoxidase (MPO), nuclear factor-kappa B (NF-kB), high mobility group box-1 (HMG B-1), total oxidant status (TOS), and total antioxidant status (TAS) levels were measured. Kidneys were analyzed histopathologically as well. Results: It was revealed that cisplatin was not effective on MPO, HMGB-1 and NF-kB levels but did increase TOS levels and decrease TAS levels in tissue samples. Interestingly, NAC elevated MPO and HMGB-1 levels significantly. Nevertheless, NAC ameliorated histological and functional changes in kidney tissues. Conclusion: It is suggested that inflammation has a limited effect on cisplatin nephrotoxicity in this experimental design, and, as reflected by decreased BUN and creatinine levels, NAC can be used as an additional therapeutic agent in standard cisplatin treatment protocols.
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    The Relationship Between Plasma Gelsolin Levels and Myeloperoxidase in Patients Undergoing Hemodialysis: A Prospective, Observational, Controlled Study
    (Erciyes Univ Sch Medicine, 2024) Tural, Haci Huseyin; Kose, Kader; Gunturk, Inayet; Kocyigit, Ismail; Yazici, Cevat
    Objective: Given the association of inflammatory conditions with the development of comorbidities, particularly cardiovascular diseases, it is crucial to monitor inflammation in hemodialysis patients. This study aimed to evaluate plasma gelsolin and myeloperoxidase (MPO) levels before and after dialysis sessions and to assess their relationship with inflammation. Materials and Methods: The study included 16 healthy volunteers and 30 patients receiving regular hemodialysis treatment. Along with routine biochemical analyses, plasma gelsolin and MPO levels were measured in blood samples taken from the study group before and after the sessions. Results: Plasma gelsolin levels were found to be statistically higher both before and after dialysis compared to the control group (p=0.000); however, there was no significant change during the session (p=0.094). Conversely, plasma MPO activity, which was significantly higher before dialysis, increased at the end of the session (p=0.000). Conclusion: It can be concluded that elevated levels of gelsolin are associated with a chronic inflammatory response, as indicated by high -sensitivity C -reactive protein (hsCRP) and MPO levels. Consequently, gelsolin could be considered a supportive treatment strategy for these patients.