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Öğe Changes of photosynthetic pigments and phytol content at different levels of nitrogen fertilizer in Italian ryegrass fresh herbage and hay(Wiley, 2022) Lv, Renlong; Elsabagh, Mabrouk; Obitsu, Taketo; Sugino, Toshihisa; Kurokawa, Yuzo; Kawamura, KensukeThe content of photosynthetic pigments in herbage not only has a strong antioxidant function, but also can improve the health of animals and the quality of livestock products after being ingested by ruminants. Increasing the content of photosynthetic pigments can improve the feed value of herbage, which is of great significance in the process of animal feeding. The experiment aimed to investigate the effect of nitrogen (N) fertilizer levels on the contents of photosynthetic pigments and phytol in fresh Italian ryegrass (IR) herbages and their changes after drying under natural condition over two consecutive seasons. IR variety: Ace (for 2013-2014 season) and Inazuma (for 2015-2016 season) were planted in 3 blocks (4 x 6 m) from two separate fields (>= 1 ha), three plots (2 x 2 m) were arranged in each block and three fertilization treatments, control: 0 kg N/ha, 60 N: 60 kg N/ha, and 120 N: 120 kg N/ha, were applied for each block. The results revealed that the crude protein, ether extract, photosynthetic pigments and phytol in IR (fresh herbage and hay) were increased with increasing N fertilizer levels. Photosynthetic pigments decreased obviously by hay preparation (chlorophylls: 40%-70%, phytol: 25%-47%, beta-carotene: 72%-90%, lutein: 31%-69%). The decomposition of phytol and beta-carotene was not affected by the growing year and N fertilizer levels. However, the decomposition of chlorophyll and lutein was affected by growing year, which was higher in 2014 than that in 2016. Also, the contents of total phytol and lutein of the fresh herbage and hay harvested in 2016 were higher (p < .05) than those harvested in 2014. In conclusion, higher N fertilizer levels could increase the photosynthetic pigments and phytol content in IR fresh herbage which contributes to improving the feed value. However, the content of photosynthetic pigments and phytol in IR hay decreased significantly. IR variety may also affect the nutrient content and their degree of decomposition during drying.Öğe Effect of phytol in forage on phytanic acid content in cow's milk(Asian-Australasian Assoc Animal Production Soc, 2021) Lv, Renlong; Elsabagh, Mabrouk; Obitsu, Taketo; Sugino, Toshihisa; Kurokawa, YuzoObjective: Bioactive compounds in ruminant products are related to functional compounds in their diets. Therefore, this study aimed to explore the effect of forage sources, Italian ryegrass (IR) silage vs corn silage (CS) in the total mixed ration (TMR), on milk production, milk composition, and phytanic acid content in milk, as well as on the extent of conversion of dietary phytol to milk phytanic acid. Methods: Phytanic acid content in milk was investigated for cows fed a TMR containing either IR silage or CS using 17 cows over three periods of 21 days each. In periods 1 and 3, cows were fed CS-based TMR (30% CS), while in period 2, cows were fed IR silage-based TMR (20% IR silage and10% CS). Results: The results showed that there were no differences in fat, protein, lactose, solidsnot-fat, somatic cell count, and fatty acid composition of milk among the three experimental periods. There were no differences in the plasma concentration of glucose, triglycerides, total cholesterol, and nonesterified fatty acids among the three experimental periods, while the blood urea nitrogen was higher (p<0.05) in period 2. The milk phytanic acid content was higher (p<0.05) in period 2 (13.9 mg/kg) compared with periods 1 (9.30 mg/kg) and 3 (8.80 mg/kg). Also, the phytanic acid content in the feces was higher (p<0.05) in period 2 (1.65 mg/kg dry matter [DM]) compared with period 1 (1.15 mg/kg DM), and 3 (1.17 mg/kg DM). Although the phytol contents in feces did not differ among the three feeding periods, the conversion ratio from dietary phytol to milk phytanic acid was estimated to be only 2.6%. Conclusion: Phytanic acid content in cow's milk increases with increasing phytol content in diets. However, phytol might not be completely metabolized in the rumen and phytanic acid, in turn, might not be completely recovered into cow's milk. The change of phytanic acid content in milk may be positively correlated with the change of phytol in the diet within a short time.Öğe Effect of varying fermentation conditions with ensiling period and inoculum on photosynthetic pigments and phytol content in Italian ryegrass (Lolium multiflorum Lam.) silage(Wiley, 2020) Lv, Renlong; Elsabagh, Mabrouk; Obitsu, Taketo; Sugino, Toshihisa; Kurokawa, Yuzo; Kawamura, KensukeThis study aimed to investigate the effect of an ensiling period (Experiment 1) and adding lactic acid bacteria (LAB, Experiment 2) on the changes in carotenoid, chlorophyll, and phytol in ensiled Italian ryegrass (IR, Lolium multiflorum Lam.). In Experiment 1, the IR herbage ensiled into plastic bags was analyzed for the contents of photosynthetic pigments and phytol over a 5-week period. During the ensiling process, the beta-carotene content decreased (p < .05), whereas the lutein content did not change. Although the chlorophyll content decreased (p < .05) after ensiling, the phytol content barely changed until week 5. In Experiment 2, IR herbage was ensiled without additive, as a Control, or with LAB for 60 days. The pH was lower (p < .05) and lactic acid content was higher (p < .05) for the LAB silage than for the Control. The chlorophyll content in silage was not affected by the LAB; however, the beta-carotene content was higher (p < .05) for the LAB silage than for the Control. Phytol and lutein contents in the herbage did not change after ensiling. These results indicate that phytol and lutein in IR herbage can be preserved well in silage, irrespective of their fermentation condition.












