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Öğe Apoptotic and cytotoxic effect of origanum minutiflorum on NB2A neuroblastoma cells(University of Ankara, 2020) Sari, Ismail; Donmez Arat, Gonca; Ozkan, Oktay; Aydemir, Isil; Ozbey, Caner; Tu?lu, M. IbrahimObjective: The aim of the present study is to investigate the cytotoxic and apoptotic effects of oregano oil from Origanum minutiflorum, which is an endemic medicinal plant in Turkey, on NB2a neuroblastoma cells. Material and Method: Cell proliferation, apoptosis, and expression of inducible and endothelial nitric oxide synthase have been determined by MTT, TUNEL, and immunohistochemistry, respectively. Result and Discussion: The cell viability was significantly decreased gradually in NB2a cells when the concentrations of both oregano oil and doxorubicin were increased. IC50doses were found to be10.75 ?l/ml for oregano oil and 5?M for doxorubicin. Furthermore, expression of inducible and endothelial nitric oxide synthase, also the number of apoptotic cells was significantly enhanced in NB2a cells after treatment with oregano oil and doxorubicin. There was a remarkable increase in inducible and endothelial nitric oxide synthase expression levels and in the number of apoptotic cells by the dual application of these agents. In addition, oregano oil enhanced the apoptotic effect of doxorubicin. In conclusion, our results indicated that oregano oil of O. minutiflorum has a cytotoxic and apoptotic effect on NB2a cells and these effects may be related to its enhancing effects on inducible and endothelial nitric oxide synthase levels. © 2020 University of Ankara. All rights reserved.Öğe Case controlled study for determination of risk factors in abdominal wall endometriosis following a cesarean section(Sage Publications Ltd, 2022) Erdogan, Pinar; Erdogan, Aliriza; Bolat, Haci; Ozbey, CanerIntroduction: Abdominal wall endometriosis (AWE) is the presence of ectopic endometrial tissue in abdominal wall and is most frequently encountered in women with previous cesarean section (CS). The aim of this study is to evaluate the possible risk factors of AWE development. Methods: Women with previous CS and pathologically confirmed AWE were included into the study (n=33). Controls (n= 127) were randomly selected among women who had previous CS and absence of AWE were confirmed by physical examination. Clinical characteristics of the patient and the CS operation preceding AWE were recorded. Results: CS was performed before onset of labor in 87.9% in AWE and in 59.1 % of control group (p= 0.002). The antenatal BMI and weight gain during pregnancy were significantly higher in AWE patients (p < 0.0001; p = 0.002, respectively). In logistic regression model procedure duration (p= 0.039; OR= 1083), antenatal BMI (p =0.003; OR= 1254), weight gain (p=0.002; OR= 1171), and CS before spontaneous labor (p= 0.021; OR = 5169) were significant parameters for predicting AWE. Discussion: High antenatal BMI, weight gain during pregnancy, and longer duration of operation are all factors effecting subsequent AWE development. However, CS before spontaneous labor is by far the most powerful risk factor for AWE development.Öğe IN VIVO HEPATOPROTECTIVE EFFECTS OF Hypericum crenulatum BOISS ON NIMESULIDE-INDUCED TOXICITY IN MICE(Parlar Scientific Publications (P S P), 2022) Ozkan, Oktay; Ozbey, Caner; Arat, Gonca Donmez; Kart, Asim; Sari, Ismail; Aydemir, IsilPlants of Hypericaceae family including many species have been used for the treatment of various dieases such as melancholia, abdominal pain, wound, tumor and oxidative stress in folk medicine. Hypericum crenulatum Boiss is a member of this family and is found throughout Bolkar mount from Meydan Yayla to San Tepe. It is an endemic herbaceous perennial plant. It is aimed to investigate in this study whether this plant specie have a hepatoprotective properties against nimesulide-induced liver injury in mice. According to the results of the study, methanolic extract of Hypericum crenulatum Boiss showed significant differences on serum ALP and liver histopathology. However, serum AST and ALT values, TAS, TOS and OSI were not altered. Hypericum crenulatum Boiss significant improvement Boiss extract at low dose provided a in nimesulide-induced acute liver toxicity. The data indicate that methanolic extract of Hypericum crenulatum Boiss is considered to be effective against liver toxicity.Öğe Investigation of distribution of GSK-3ß signal pathway by age groups in cases of ulcerative colitis(Editura Acad Romane, 2023) Ozbey, Caner; Aydemir, IsilUlcerative colitis (UC), one of the inflammatory bowel diseases, has been reported to increase in recent years. Although the exact cause is unknown, disruptions in the molecular pathways are thought to trigger UC. We aimed to examine the distributions of glycogen synthase kinase-3beta (GSK-3 ss), nuclear factor-kappa B (NF-kappa B) and wingless/int-1 (Wnt-1) in different age groups diagnosed with UC. Patients diagnosed with UC were divided into four groups according to their ages: Group 1, aged 18-30 (n=20); Group 2, aged 31-45 (n=20); Group 3, aged 46-60 (n=20); Group 4, aged 61-75 ( n=20). Tissue sections were histochemically stained to examine the parameters of epithelial cell height, length of crypt, thickness of muscularis mucosa and extent of submucosal fibrosis. The immunohistochemistry assay was performed using cell survival and for GSK-3 ss, NF-.B and Wnt-1 cell growth markers. Immunoreactivities were evaluated using H-score and analyzed using the one-way analysis of variance (ANOVA) test for statistics. It was detected a decrease in the histopathological parameters whereas the immunoreactivities of GSK-3 ss, NF-kappa B and Wnt-1 were increased with increasing age. The levels of GSK-3 ss immunoreactivity were similar in both epithelium and submucosa in all groups. NF-.B immunoreactivity was higher in submucosa of Groups 1, 2 and 3, while Wnt-1 was enhanced in Groups 1 and 3. The results of histopathology showed that the integrity of the epithelial tissue in the colon deteriorated with increasing age. The expressions of GSK-3 ss, NF-kappa B and Wnt-1 were detected in all age groups. We thought that there was a synergistic activation between these three markers. Nevertheless, studies are needed to investigate this molecular pathway.Öğe Investigation of the effects of bisphenol-A exposure on lymphoid system in prenatal stage(Sage Publications Inc, 2020) Aydemir, Isil; Ozbey, Caner; Ozkan, Oktay; Tuglu, Mehmet Ibrahim; Kum, SadiyeBisphenol-A (BPA) used in the production of plastic materials is a temperature-soluble agent. It also has a steroid hormone-like activity; therefore, it poses a danger to human health. In our study, we aimed to investigate the effects of BPA on lymph node and spleen in male rats exposed to this agent during prenatal stage. The pregnant female rats were divided into four groups: control, sham, low dose (300 mu g/kg BPA), and high dose (900 mu g/kg BPA). BPA was dissolved in 1 mL of corn oil and administered to the pregnant rats every day during pregnancy. On the 21st and 45th day after the birth, male rats' lymph node and spleen samples were taken and histopathological examination was performed. Samples were stained with hematoxylin and eosin to determine the general histological appearance, and with CD3 and CD20 immunohistochemically. The results of staining were evaluated by H-score, and statistical analysis was performed. In the samples, BPA applications were not found to cause significant tissue damage. But there was a significant decrease in the immunoreactivities of CD3 and CD20 after BPA applications in both 21st and 45th day samples. After high dose BPA administration, decreased CD3 immunoreactivity was statistically significant. It is thought that BPA does not cause histologically significant tissue damage, but it may impair organ function at cellular level. The investigation of molecules involved in organ function will be useful in revealing the mechanisms that will cause dysfunction.
