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Öğe COATING OF CHITOSAN AND SALICYLIC ACID CAN MAINTAIN QUALITY CHARACTERISTICS OF TABLE GRAPES(Pakistan Agricultural Scientists Forum, 2023) Ozden, M.; Qaderi, R.International fresh table grape trade has expanded tremendously over the last few decades. However, fresh table grapes decay quickly due to pathogen infestation and water loss, which make it difficult to preserve without an effective treatment. Therefore, this study was conducted to investigate the effects of CT, with SA on maintaining quality characteristics of 'Red Globe' grapes during storage at 0.5 degrees C and 90-95% RH in pomology laboratories of the faculty in 2018-2019. The experiment was conducted using a completely randomized design with treatments [1% CT and two concentrations of SA (0, 1 and 2 mM)] fora 60 day of storage time. To determine the effects of the treatmentsat 15-day intervals, weight loss, fruit decay, chroma index, total soluble solids, titratable acidity (TA), pH, fruit flesh firmness and secondary metabolites such as total phenolic contents, total flavonoids and total anthocyanins were determined. Also, antioxidant activity and antioxidant capacity of the berry extracts determined by the DPPH and FRAP assays at each sampling time. Results revealed no effect on total soluble solids and total phenolics However, there were significant effects on the quality parameters fruit decay, weight loss, TA, pH, chroma index, and fruit flesh firmness. Chitosan coating was also found to be a potentially useful treatment for the phytochemical compounds and total antioxidant activity. The pre-storage IC50 value of the berry extract was 0.53 mg mL(-1) and by the end of storage period, the values were 0.49 mg mL(-1) in control, 0.50 mg mL(-1) in CT, 0.53 mg mL(-1) in CT + 1 mM SA and 0.46 mg mL(-1) in CT + 2 mM SA. Total antioxidant capacity of grapes was 23.7 mg BHT mL(-1) before storage and this increased during storage for both coated and uncoated grapes. Based on the findings, there was a strong relationship between total phenolic and flavonoid contents of the berries and antioxidant activity. It was concluded that the use of CT + 2 mM SA together with MAP is a potentially useful alternative to the use of SO2 in grape storage in commercial contexts.Öğe EFFECTS OF COLCHICINE APPLICATION AND PLOIDY LEVEL ON FRUIT SECONDARY METABOLITE PROFILES OF GOLDENBERRY (PHYSALIS PERUVIANA L.)(Aloki Applied Ecological Research And Forensic Inst Ltd, 2020) Comlekcioglu, N.; Ozden, M.The aim of this study was to investigate changes of phenolic accumulation and antioxidant potential of goldenberry fruits collected from diploid plants, under colchicine treatments of different doses and exposure time, including colchicine induced synthetic tetraploid plants. Three colchicine application methods were utilized; i) immersion of seeds in colchicine solution at concentrations of 0.0, 0.6, 0.9% for 24 h and 36 h; ii) keeping germinated seeds (with 2-3 mm long roots) in a colchicine solution with concentrations of 0.6% and 0.9% for 24 h iii) incubation of seeds on semi solid nutrient medium containing 0 6% and 0.9% colchicine for 14 and 21 days. Total antioxidant activity of fruits varied according to the dose and exposure time of colchicine applications. The most important change was observed in the fruits of plants that turned tetraploid as a result of colchicine applications. Tetraploid fruits generally produced higher amounts of secondary compounds compared to diploid progenitors and their antioxidant capacity was also higher. However, the results show that polyploidization is important but cannot be generalized as a strategy to increase secondary metabolite production. It was determined that the relationship between total anthocyanin and total phenolic content with antioxidant capacity of fruits was significant and parallel. We suggest the use of the lowest colchicine concentration tested. 0.6% colchicine to enhance metabolite production cannot be generalized.Öğe NEEM SEED OILCONTAINING METHYLCELLULOSE AS NEW POSTHARVEST ORGANIC EDIBLE COATING FOR TABLE GRAPES DURING COLD STORAGE(Aloki Applied Ecological Research And Forensic Inst Ltd, 2023) Olorunsogbon, S. T.; Ozden, M.; Riley, I. T.Table grapes have a high-water content and are non-climacteric. They are prone to degradation and infections. This study reveals the effect of neem seed oil combined with methylcellulose (NSOMC) at (control, 0.5%NSOMC, 2%NSOMC, 8%NSOMC, 32%NSOMC and 1% of methylcellulose (MC) prolonging the postharvest quality of Sultana table grapes during 60 days of storage at 0.5 & DEG;C. The different concentrations of the NSOMC were found effective compared to the control and 1%MC. NSOMC coated fruits, especially at 32% showed less decay incident (3.1%), weight loss (0.32%) and colour changes (8.49) compared to the other coated fruits and the control. 32%NSOMC coated fruits retained their total soluble content and titratable acidity with the lowest mean pH value (3.43). Phenolic compounds (total phenolic and total flavonoid content) had the highest mean and the antioxidant activity was reduced in 32%NSOMC coated fruits. Therefore, our findings suggested that the neem seed oil combined with methylcellulose is safe and could be used as a commercial treatment to increase the shelf life of table grapes. The higher the concentrations of the neem seed oil, the greater the efficiency of the treatments.Öğe Polyploidy induction by colchicine treatment in golden berry (Physalis peruviana), and effects of polyploidy on some traits(Pakistan Agricultural Scientists Forum, 2019) Comlekcioglu, N.; Ozden, M.Goldenberry (Physalis peruviana L) an important small fruit that is growing rapidly around the globe. Different colchicine treatments (0.0, 0.3, 0.6, and 0.9% w/v) were used to induce tetraploidy in goldenberry plants. Three colchicine treatment methods were evaluated; i) immersion of seeds in colchicines solution, ii) immersion of germinated seeds (with roots about 2 mm long) in colchicine and iii) incubation of seeds on semi solid Murashige and Skoog (MS, 1962) medium containing colchicines. Experiments were set up with three factors factorial experimental design with three replicates, where the colchicine application method was the main factor of variation, the concentrations was the secondary factor and the exposure time was the third factor. Twenty seeds for each treatment, with three replications were used. Diploid plants grown from untreated seeds were used as controls. The incubation of seeds on colchicine containing MS medium was found to be the only effective method and tetraploid plants were produced in this way. The highest tetraploidy was achieved by adding 0.06 and 0.09% colchicine to MS medium. An exposure time 21-30 d was sufficient to produce tetraploid plants. Ploidy determination was made by flow cytometry. The polyploidization rate ranged between 4.1-58.8%. Tetraploid plant could not be obtained from other treatments. Statistical analysis was not performed since data could not be obtained from the lowest groups. The induced tetraploid plants were with larger plants, fruits, leaves, and stomata, higher chlorophyll content and lower stomata density compared to diploid control plants. The results have shown that colchicine should be added to the in vitro culture medium at least 0.6% and 0.9% doses and the seeds should be cultured for at least 30 days. An analysis of variance (two sample T-test) was performed at the 5% significance level to comparison of the morphologic characteristics of diploid and tetraploid plants. © 2019, Pakistan Agricultural Scientists Forum. All rights reserved.Öğe POLYPLOIDY INDUCTION BY COLCHICINE TREATMENT IN GOLDEN BERRY (PHYSALIS PERUVIANA), AND EFFECTS OF POLYPLOIDY ON SOME TRAITS(Pakistan Agricultural Scientists Forum, 2019) Comlekcioglu, N.; Ozden, M.Goldenberry (Physalis peruviana L) an important small fruit that is growing rapidly around the globe. Different colchicine treatments (0.0, 0.3, 0.6, and 0.9% w/v) were used to induce tetraploidy in goldenberry plants. Three colchicine treatment methods were evaluated; i) immersion of seeds in colchicines solution, ii) immersion of germinated seeds (with roots about 2 mm long) in colchicine and iii) incubation of seeds on semi solid Murashige and Skoog (MS, 1962) medium containing colchicines. Experiments were set up with three factors factorial experimental design with three replicates, where the colchicine application method was the main factor of variation. the concentrations was the secondary factor and the exposure time was the third factor. Twenty seeds for each treatment, with three replications were used. Diploid plants grown from untreated seeds were used as controls. The incubation of seeds on colchicine containing MS medium was found to be the only effective method and tetraploid plants were produced in this way. The highest tetraploidy was achieved by adding 0.06 and 0.09% colchicine to MS medium. An exposure time 21-30 d was sufficient to produce tetraploid plants. Ploidy determination was made by flow cytometry. The polyploidization rate ranged between 4.1-58.8%. Tetraploid plant could not be obtained from other treatments. Statistical analysis was not performed since data could not be obtained from the lowest groups. The induced tetraploid plants were with larger plants, fruits, leaves, and stomata, higher chlorophyll content and lower stomata density compared to diploid control plants. The results have shown that colchicine should be added to the in vitro culture medium at least 0.6% and 0.9% doses and the seeds should be cultured for at least 30 days. An analysis of variance (two sample T -test) was performed at the 5% significance level to comparison of the morphologic characteristics of diploid and tetraploid plants.