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    GENOTYPIC IDENTIFICATION AND CHARACTERISATION OF PROBIOTIC PROPERTIES OF LACTIC ACID BACTERIA FROM NIGDE CHEESE
    (SCIBULCOM LTD, 2016) Ilk, S.; Canpolat, E.; Ozgen, M.; Saglam, N.; Sultana, N.
    Probiotics are live microorganisms having profound health benefit on its host and among them lactic acid bacteria (LAB) are common group found in human food. As this is a very vast group of microorganisms having different strains, therefore genotypic identification has been performed by primers that are specific for 16s ribosomal DNA gene on a total of 24 exopolysaccharides (EPS) producing LAB: those are Lactobacillus, Enterococcus and Pediococcus isolated from Nigde cheese of Turkey. Subsequently, characterization of probiotic properties of LAB was studied by final pH, acidification, and amount of EPS concentration and aggregation properties. EPS productions by the strains were found between 54.1-93.6 mg/l. The highest EPS production was detected in the Pediococcus pentosaceus EC18 and the lowest EPS were estimated in the Enterococcus faecium EC26. All LAB strains showed auto- and coaggregation ability with Escherichia coli (E. coli) ATCC 25923 and Salmonella enterica serotype typhmurium (S. typhmurium) SL 1344. Results showed that the aggregation property is specific for each strain and dependent on EPS production. Our results indicate that the ability to autoaggregate, together with exopolysaccharide and final pH properties and coaggregation abilities with E. coli and S. typhmurium strains, can be used for preliminary screening in order to identify potentially probiotic bacteria suitable for human.
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    Genotypic identification and characterisation of probiotic properties of lactic acid bacteria from nigde cheese
    (Scibulcom Ltd., 2016) Ilk, S.; Canpolat, E.; Ozgen, M.; Saglam, N.; Sultana, N.
    Probiotics are live microorganisms having profound health benefit on its host and among them lactic acid bacteria (LAB) are common group found in human food. As this is a very vast group of microorganisms having different strains, therefore genotypic identification has been performed by primers that are specific for 16s ribosomal DNA gene on a total of 24 exopolysaccharides (EPS) producing LAB: those are Lactobacillus, Enterococcus and Pediococcus isolated from Nigde cheese of Turkey. Subsequently, characterization of probiotic properties of LAB was studied by final pH, acidification, and amount of EPS concentration and aggregation properties. EPS productions by the strains were found between 54.1–93.6 mg/l. The highest EPS production was detected in the Pediococcus pentosaceus EC18 and the lowest EPS were estimated in the Enterococcus faecium EC26. All LAB strains showed auto-and coaggregation ability with Escherichia coli (E. coli) ATCC 25923 and Salmonella enterica serotype typhmurium (S. typhmurium) SL 1344. Results showed that the aggregation property is specific for each strain and dependent on EPS production. Our results indicate that the ability to autoaggregate, together with exopolysaccharide and final pH properties and coaggregation abilities with E. coli and S. typhmurium strains, can be used for preliminary screening in order to identify potentially probiotic bacteria suitable for human. © 2016, Scibulcom Ltd. All rights reserved.
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    Performance of 'Fuji' Apple on M.9 Rootstock in Different Tree Training Systems for the First Five Years
    (TARBIAT MODARES UNIV, 2016) Ozkan, Y.; Yildiz, K.; Kucuker, E.; Cekic, C.; Ozgen, M.; Akca, Y.
    The effects of five training systems on tree growth, yield, and some fruit characteristics were assessed for 'Fuji' apple grafted on M.9 rootstock for the first five years in Tokat, Turkey. The trees were trained in one of five ways: Slender Spindle (SS, 4762 trees ha(-1)), Vertical Axis (VA, 2857 trees ha(-1)), HyTec (HT, 1904 trees ha(-1)) and two different tree densities of super spindle (L-Sup S with 5,000 trees ha(-1); H-Sup S with 10.000 trees ha(-1)). Trunk Cross-sectional Area (TCA), canopy diameter and canopy volume were higher in low tree density systems (HT and VA) than in high tree density systems (SS, L-Sup S and H-Sup S). Annual and cumulative yields per tree over the first cropping years were higher in VA and HT than in SS, L-Sup S or H-Sup S. Yield per unit area was the highest in H-Sup S in every year due to the higher number of trees per hectare. Yield efficiency (yield cm(-2) TCA) was higher in VA and HT than in SS, L-Sup S or H-Sup S in every year. HT produced the largest fruit among the training systems in every year. VA had the second largest fruit in 2008, 2010, and 2011.