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    Investigating effect of miR160 through overexpression in potato cultivars under single or combination of heat and drought stresses
    (Springer, 2021) Sanli, Beyazit Abdurrahman; Ozturk Gokce, Zahide Neslihan
    Potato (Solanum tuberosum L.) is one of the major tuber crops which is widely grown in the world. Potato growth and development as well as yield is negatively affected by heat and drought stress alone, but combined stress has devastating effect. MicroRNAs are group of small RNAs which play vital role in gene regulation at transcriptional and post-transcriptional level by repressing or inhibiting protein expression of corresponding target. Current study aimed to understand the function of miR160a-5p and its influence on its target gene ARF16 under applied stresses. Potato cultivars tolerant (Unica) and sensitive (Russet Burbank) were tested under drought, heat, and their combination of stresses. The expression of miR160a-5p was revealed to be higher along with the suppression of corresponding target gene ARF16 gene. Transgenic plants also showed improved physiological, and biochemical traits, with lower accumulation of H2O2 and higher levels of proline solely under drought stress conditions. It was also observed that overexpression of miR160a-5p could alter mechanisms for auxin response factor pathways which enabled transgenic plants to have higher tolerance to abiotic stress conditions. Based on our findings, transgenic Unica and Russet Burbank plants showed increased tolerance to drought and heat stress as compared to wild-type plants. This study can be helpful in understanding the role of miR160a-5p in improved tolerance to abiotic stresses.
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    Investigation of the effects of overexpression of Novel_105 miRNA in contrasting potato cultivars during separate and combined drought and heat stresses
    (Tubitak Scientific & Technological Research Council Turkey, 2021) Yalcin, Melis; Ozturk Gokce, Zahide Neslihan
    Potato (Solanum tuberosum L.) growth, development and production are sensitive to abiotic stress conditions, yet studies about investigation of stress response of potato plants in regulation of gene expression level are limited. The aim of this study was to identify the role of newly identified Novel_105 miRNA in contrasting potato cultivars (tolerant Unica and sensitive Russet Burbank) in response to heat, drought and combined heat and drought stresses using transgenic approach. Transgenic plants of Unica and Russet Burbank were generated with overexpression of pre-miRNA of Novel_105 and both wild type and transgenic T0 lines were subjected to stress. Physiological parameters (gaseous exchange, leaf temperature, chlorophyll content and relative water content) were observed along with biochemical parameters including proline, malondialdehyde and hydrogen peroxide. The overexpression of Novel_105 improved physiobiochemical functions of the both cultivars under single or combined stress conditions. The increase in Novel_105 miRNA expression was observed along with a decreased expression in its predicted target E3 ubiquitin-protein ligase XBAT35, that is responsible for cell death in potato. This is a preliminary study for the development of abiotic stress resilient potato cultivars proving the overexpression of Novel_105 miRNA increased abiotic stress tolerance presumably by repressing the expression of XBAT35 responsible from promoting cell death.