Yazar "Sajid, Qurat" seçeneğine göre listele

Listeleniyor 1 - 1 / 1
  • Küçük Resim Yok
    Öğe
    Investigation of Virus Diseases and Molecular Detection of Little Cherry Virus 1 on Cherry Plants at Niğde Province
    (2019) Sajid, Qurat; Elçi, Eminur
    To investigate the virus infections of sour and sweet cherries, various locations of Niğde provincewere examined during 2017. Ninety sweet and sour cherry leaf samples showing suspicious virussymptoms were collected and screened with virus-specific primers: Little cherry virus 1 (LChV1),Cherry necrotic rusty mottle virus (CNRMV), Prune dwarf virus (PDV), Prune necrotic ring spotvirus (PNRSV), Apple mosaic virus (ApMV), Cherry green ring mottle virus (CGRMV), Cherryleaf roll virus (CLRV), Cherry mottle leaf virus (CMLV), Plum bark necrotic stem pitting associatedvirus (PBNSPaV), Cherry twisted leaf virus (CTLV), Apple stem grooving virus (ASGV), Littlecherry virus 2 (LChV2), Cherry rusty leaf virus (CRLV), Apple stem pitting virus (ASPV), Applechlorotic leaf spot virus (ACLSV). Based on RT-PCR analysis, no amplification was observedexcept LChV1 amplifications, dsRNA analysis resulted in one suspicious profile. To validate thoseresults, more sensitive TaqMan Real-Time PCR analysis and sequence analysis were conducted andLChV1 was detected on 7 samples. It can be concluded that only a low quantity of LChV1 infectionswas observed on some of the screened cherry samples.