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    Antioxidant capacity and chemical properties of selected barberry (Berberis vulgaris L.) fruits
    (KOREAN SOC HORTICULTURAL SCIENCE, 2012) Ozgen, Mustafa; Saracoglu, Onur; Gecer, Esma Nur
    Six purple-black barberry accessions (Berberis vulgaris L.) were obtained from various sites in Sivas province of Turkey. Fruits were analyzed for their total phenolic (TP), total monomeric anthocyanin (TMA) contents and for their antioxidant capacity by the ferric reducing antioxidant power (FRAP) and TEAC assays. Sugar and organic acid composition were also determined by HPLC. TP and TMA contents were measured using the Folin-Ciocalteu reagent and pH differential methods, respectively. Variability between the accessions was not high. Variability among accessions was greatest for FRAP content (C.V. 17.9%); individuals ranged from 41.0 to 65.6 mu mol TE center dot g(-1) on a fresh weight basis. Variation among the accessions was also evident for TP, TMA, and TEAC values (C.V. 16.21%, 14.85%, and 7.13%, respectively). The major sugars of barberry fruits were glucose (8.84 g center dot 100 mL(-1)) and fructose (6.12 g center dot 100 mL(-1)) and dominant organic acids were malic (7.59 g center dot 100 mL(-1)) and citric (1.34 g center dot 100 mL(-1)). Within this limited study of six different accessions, chemical profile and antioxidant capacity of barberry fruits have rich source of natural antioxidant substances.
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    Antioxidant potential of Turkish pepper (Capsicum annuum L.) genotypes at two different maturity stages
    (TUBITAK SCIENTIFIC & TECHNICAL RESEARCH COUNCIL TURKEY, 2016) Keles, Davut; Ozgen, Senay; Saracoglu, Onur; Ata, Atilla; Ozgen, Mustafa
    Improved phytochemicals and antioxidant properties in crops are becoming important traits in many breeding programs. In this study, along with several other horticultural attributes, total phenolic content (TP) and antioxidant capacity (ferric reducing ability of plasma (FRAP) and Trolox equivalent antioxidant capacity (TEAC)) of 52 superior pepper genotypes from the Alata Pepper Breeding Program were examined. The fruits from these plants were harvested at immature and mature stages. The genotypes greatly varied for TP, FRAP, TEAC, soluble solids, vitamin C content, and fruit color as determined by L, a and b values. The range for TP was 319-4047 mu g GAE/g fresh weight (fw), while FRAP and TEAC varied between 0.22 and 0.56 mu mol of TE/g fw and 0.08 and 1.88 mu mol TE/g fw. All these characteristics were considerably variable between immature and mature stages. These characteristics were also found to be significantly correlated. Principal component analyses conducted for all the characters used in the study and constructed separately for immature and mature stages demonstrated no obvious patterns for pepper types. Therefore, our overall results suggest that individual pepper cultivars having high total phenolic and antioxidant capacity can be utilized in developing new pepper cultivars with rich phytochemical content.
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    LESS KNOWN VACCINIUM: ANTIOXIDANT AND CHEMICAL PROPERTIES OF SELECTED CAUCASIAN WHORTLEBERRY (Vaccinium arctostaphylos) FRUITS NATIVE TO BLACK SEA REGION OF TURKEY
    (WYDAWNICTWO AKAD ROLNICZEJ W LUBLINIE, 2014) Ozgen, Mustafa; Celik, Huseyin; Saracoglu, Onur
    There is an increasing interest in the commericalization of native fruits for food and medicinal extract utilization. This study was undertaken to determine the antioxidant and chemical properties of selected caucasian whortleberry from the native region of Northeastern Turkey. Caucasian whortleberry (Vaccinium arctostaphylos L.), a close relative to cultivated blueberries, were harvested from various sites of Black sea region of Turkey and were analyzed for their total phenolic (TP), anthocyanin (TA) contents, and their antioxidant capacity by FRAP and TEAC assays. Specific sugar and organic acid composition were also determined by the help of HPLC. ARC-1, and ARC-6 displayed the highest TP contents (5780 and 5754 mu g GAB g(-1) fw). The TA values were spectrophotometrically estimated and they were varied between 74.7 (ARC-6) and 194.4 (ARC-4) mu g del-3-glu g(-1) fresh weight basis. ARC-1 had the highest amount (19.5 and 23.4 mu mol TE g(-1) fw) of antioxidant capacity or both TEAC and FRAP assays. Fructose and glucose were found to be predominant sugars in all genotypes analyzed. The concentrations of fructose and glucose were averaged at 45.1 and 41.2 g kg(-1) fw respectively. The organic acid distribution of berries was dominated by citric acid (mean of 9.85 g kg(-1) fw). These results may provide evidence that Caucasian whortleberry has strong antioxidant capacity. Also, results of this study may be used for utilization of selected genotypes in the breeding studies of close relative vaccinium plants.