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Öğe A significant association between rs2295190 polymorphism of the ESR1 gene and fibromyalgia syndrome(C M B Assoc, 2022) Kaydok, Ercan; Ozmen, Esma; Sari, Ismail; Sen Cakiroglu, Gozde; Tas, Ayca; Silig, Yavuz; Hayta, EmrullahFibromyalgia syndrome (FMS) is a multifactorial disease characterized by chronic diffuse pain. Genetic factors are also involved in the etiology. However, there is not enough information on the genetic factors that play a role in the pathogenesis of FMS. This study aims to investigate the relationship between estrogen receptor 1 gene (ESRI) 594G>A (rs2228480) and 325C>G (rs2295190) polymorphisms and FMS. A total of 294 women, 146 of who were FMS patients and 148 of whom were healthy controls, were enrolled in the study. The instruments used to collect data from patients included patient follow-up forms, Visual Analog Scale (VAS), and Fibromyalgia Impact Questionnaire (FIQ). Genotyping of ESR1 594G>A and 325C>G polymorphisms in the extracted DNA samples was performed using an RT-PCR device and TaqMan hydrolysis probes. it was found that, for rs2295190 polymorphism, patients with CO and GO genotypes versus CC genotypes showed a decreased risk for FMS (OR: 0.442; 95% CI: 0.234-0.833). But there were no significant differences were found in the genotype distribution of rs2228480 polymorphism between the FMS patients and controls. The intragroup evaluation of FMS patients revealed no significant association between symptoms, pain score, FIQ score, and polymorphisms (p>0.05). We believe that there is a significant association between ESR1 rs2295190 polymorphism and FMS and that this polymorphism may be protective against FMS. However, there is a need for comprehensive studies on different populations to obtain clearer data as well as further studies to elucidate the possible mechanism of association. Copyright: (C) 2022 by the C.M.B. Association. All rights reserved.Öğe Alteration of butyrylcholinesterase level in cholelithiasis patients after laparoscopic cholecystectomy: Do butyrylcholinesterase levels affect lipid metabolism?(Bayrakol Medical Publisher, 2021) Ayan, Durmus; Bolat, Haci; Ozmen, Esma; Sari, IsmailAim: Cholelithiasis (gallbladder stone) is a disease with a high incidence worldwide. The disease is multifactorial and various factors such as gender, age, obesity and use of oral contraceptives are held responsible for the development of the disease. In addition, lipid disorder is observed in more than 50% of patients with cholelithiasis. Laparoscopic cholecystectomy (LC) is one of the most frequently used surgical methods in the treatment of cholelithiasis, and there are data indicating that lipid profile changes and metabolic syndrome (MetS) develop after the operation. In this study, we aimed to investigate whether there is a change in lipid profile and Butyrylcholinesterase (BChE) activity, which affects lipid metabolism, in cholelithiasis patients after LC. Material and Methods: In our study, 31 patients (obese and non-obese) who applied to the general surgery clinic of Nigde Omer Halisdemir University Training and Research Hospital were included. Blood samples were taken from the patients before LC and 6 months after the operation, and the lipid levels and BChE enzyme activity were examined using spectrophotometric method. Results: According to the results, it was determined that the total cholesterol (p=0.015) and LDL (p=0.010) levels significantly decreased after LC, while no significant difference was found in the other parameters examined (p>0.05). In addition, it was observed that there was no significant correlation between the lipid profile examined both before and after LC and BChE activity level. Discussion: According to the data obtained, the significant decrease in LDL and total cholesterol after LC indicates that this operation causes a positive change at least within a period of 6 months in the lipid profile of patients.Öğe Apoptotic and cytotoxic effect of origanum minutiflorum on NB2A neuroblastoma cells(University of Ankara, 2020) Sari, Ismail; Donmez Arat, Gonca; Ozkan, Oktay; Aydemir, Isil; Ozbey, Caner; Tu?lu, M. IbrahimObjective: The aim of the present study is to investigate the cytotoxic and apoptotic effects of oregano oil from Origanum minutiflorum, which is an endemic medicinal plant in Turkey, on NB2a neuroblastoma cells. Material and Method: Cell proliferation, apoptosis, and expression of inducible and endothelial nitric oxide synthase have been determined by MTT, TUNEL, and immunohistochemistry, respectively. Result and Discussion: The cell viability was significantly decreased gradually in NB2a cells when the concentrations of both oregano oil and doxorubicin were increased. IC50doses were found to be10.75 ?l/ml for oregano oil and 5?M for doxorubicin. Furthermore, expression of inducible and endothelial nitric oxide synthase, also the number of apoptotic cells was significantly enhanced in NB2a cells after treatment with oregano oil and doxorubicin. There was a remarkable increase in inducible and endothelial nitric oxide synthase expression levels and in the number of apoptotic cells by the dual application of these agents. In addition, oregano oil enhanced the apoptotic effect of doxorubicin. In conclusion, our results indicated that oregano oil of O. minutiflorum has a cytotoxic and apoptotic effect on NB2a cells and these effects may be related to its enhancing effects on inducible and endothelial nitric oxide synthase levels. © 2020 University of Ankara. All rights reserved.Öğe Are VEGF and SCUBE1 gene expressions increased in diabetic retinopathy?(Walter De Gruyter Gmbh, 2023) Ayan, Durmus; Zor, Kuersad Ramazan; Ozmen, Esma; Bicer, Gamze Yildirim; Onder, Cagatay Emir; Sari, IsmailObjectives: In the current study, we synergistically evaluated vascular endothelial growth factor (VEGF) gene expression levels and signal peptide-CUB-EGF domain-containing protein 1 (SCUBE1) gene expression levels in diabetic patients without retinopathy, non-proliferative diabetic retinopathy (NPDR), and proliferative diabetic retinopathy (PDR).Methods: 94 blood samples from 26 healthy controls, 29 non-DR, 22 NPDR, and 17 PDR patients were collected in sterile EDTA tubes. Total RNA was obtained from these samples without waiting and then converted to cDNA. The expression levels of the VEGF and SCUBE1 genes were determined by quantitative real-time polymerase chain re-action (qPCR).Results: SCUBE1 gene expression levels were 2.15 (p=0.015), 1.75 (p=0.799), 2.37 (p=0.037) times higher, and VEGF gene expression levels were 1.71 (p=0.023), 1.75 (p=0.012), 1.85 (p=0.031) times higher in the non-DR, NPDR, and PDR groups compared to the control group, respectively. VEGF gene expression levels were significantly higher in participants with HbA1c levels >= 5.7% compared to those with < 5.7. SCUBE1 and VEGF gene expression levels were significantly higher in participants with fasting plasma glucose (FPG) levels >= 126 mg/dL than those with < 126 mg/dL.Conclusions: As a result, SCUBE1 gene expression levels are higher than VEGF gene expression levels, especially in the PDR group. Therefore, SCUBE1 may contribute to the pathology of DR just like VEGF by generating angiogenesis. However, we believe there isa need for experimental animal model studies with DR examining SCUBE1 gene expression levels in tissue samples.Öğe ASSOCIATION OF THE SEH GENE PROMOTER POLYMORPHISMS AND HAPLOTYPES WITH PREECLAMPSIA(Soc Medical Biochemists Serbia, 2020) Sari, Ismail; Okten, Hatice; Aktan, Cagdas; Cihan, EsraBackground: The epoxyeicosatrienoic acids (EETs) have antihypertensive, anti-inflammatory, and organ protective properties and their circulation levels are related to hypertension, diabetes mellitus, cardiovascular diseases, and preeclampsia. Soluble epoxide hydrolase (sEH) catalyses the degradation of EETs to less biologically active dihydroxyeicosatrienoic acids. Here, we sequenced the promoter region of EPHX2 to investigate the association between promoter sequence alterations that we thought to affect the expression levels of the enzyme and preeclampsia (PE). Methods: Nucleotide sequencing of the promoter region of the EPHX2, spanning from position -671 to +30, was performed on 100 pregnant women with PE and, 20 or more weeks pregnant normotensive, healthy women (n=100). Results: Pregnant women who carry rs4149235, rs4149232, rs73227309, and rs62504268 polymorphisms have 4.4, 2.4, 2.3, and 2.8 times significantly increased risk of PE, respectively. CCGG (OR: 3.11; 95% CI: 1.12-8.62) and CCCA (OR: 0.45; 95% CI: 0.36-0.55) haplotypes were associated with an increased and decreased risk of PE, respectively. Conclusions: Four SNPs (rs4149232, rs4149235, rs73227309, and rs62504268) in the promoter region of the EPHX2, and CCGG and CCCA haplotypes of these 4 SNPs were significantly associated with PE. These SNPs in the promoter region may affect sEH expression and enzyme activity and may play a role in PE pathogenesis by causing individual differences in EET levels. However, future studies are needed to confirm our findings and examine the effect of these SNPs on the sEH expression and/or enzyme activity.Öğe Effect of ovarian stimulation on the expression of piRNA pathway proteins(Public Library Science, 2020) Sari, Ismail; Gumus, Erkan; Taskiran, Ahmet Sevki; Sokmensuer, Lale KarakocPIWI-interacting RNAs (piRNAs) play an important role in gametogenesis, fertility and embryonic development. The current study investigated the effect of different doses of pregnant mare serum gonadotrophin/human chorionic gonadotrophin (PMSG/hCG) and repeated ovarian stimulation (OS) on the expression of the Mili, Miwi, Mael, Tdrd1, Tdrd9, qnd Mitopld genes, which have crucial roles in the biogenesis and function of piRNAs. Here, we found that after treatment with 7.5 I.U. PMSG/hCG and two repeated rounds of OS, both the mRNA and protein levels of Tdrd9, Tdrd1 and Mael showed the greatest decrease in the ovarian tissue, but the plasma E-2 levels showed the strongest increases (p< 0.05). However, we found that the Mitopld, Miwi and Mili gene levels were decreased significantly after treatment with 12.5 I.U. PMSG/hCG. Our results suggested that exogenous gonadotropin administration leads to a significant decrease in the expression of the Mili, Miwi, Mael, Tdrd1, Tdrd9 and Mitopld genes, which are critically important in the piRNA pathway, and the changes in the expression levels of Tdrd9, Tdrd1 and Mael may be associated with plasma E-2 levels. New comprehensive studies are needed to reduce the potential effects of OS on the piRNA pathway, which silences transposable elements and maintains genome integrity, and to contribute to the safety of OS.Öğe EVALUATION OF OXIDATIVE STRESS, 3-NITROTYROSINE, AND HMGB-1 LEVELS IN PATIENTS WITH WET TYPE AGE-RELATED MACULAR DEGENERATION(Soc Medical Biochemists Serbia, 2022) Zor, Kursad Ramazan; Sari, Ismail; Bicer, Gamze Yildirim; Gunturk, Inayet; Kucuk, Erkut; Ersan, Serpil; Seydel, Gonul SeydaBackground: This study aims to compare serum HMGB-1, 3-nitrotyrosine (3-NT), TAS, TOS, and OSI levels in Wettype Age-Related Macular Degeneration (wAMD) patients and healthy controls to determine the correlation of these parameters with each other. Methods: Thirty patients with Wet-type Age-Related Macular Degeneration (wAMD) and 27 healthy adults, as controls were enrolled in the study. We determined the TAS and TOS levels in serum samples of both groups using commercial kits on a microplate reader. Serum HMGB-1 and 3-NT levels were measured with the enzyme-linked immunosorbent assay method. Results: HMGB-1 levels were significantly higher in the patient group (137.51 pg/mL, p=0.001), while there was no difference between the two groups in serum 3-NT levels (p = 0.428). A statistically significant difference found in the levels of TOS and OSI (p = 0.001 and p = 0.045, respectively) between the patients and controls, however, no significant difference was observed between the groups in terms of TAS levels (p = 0.228). Conclusions: Oxidative stress and HMGB-1 levels were increased in wAMD patients and enhanced oxidative stress may be associated with increased tissue necrosis and inflammation. Thus administration of antioxidant treatment in addition to routine therapy should be considered in wAMD.Öğe Examination of the relationship between variants in the gene region encoding soluble epoxy hydrolase enzyme hydrolytic activity and type 2 diabetes(Bayrakol Medical Publisher, 2024) Ozmen, Esma; Ayan, Durmus; Onder, Cagatay Emir; Akin, Dilara Fatma; Kose, Burcu; Sari, Ismail; Yazici, CevatAim: Epoxyeicosanoids function as signal mediators in critical biological processes such as platelet aggregation, vasodilation, and anti -inflammation. With all these properties, Epoxyeicosanoids have been associated with many diseases. Metabolism of epoxyeicosanoids is carried out by soluble epoxide hydrolase enzymes, and as a result dihydroxyeicosatrienoic acids, which is a less active form than epoxyeicosanoids, are formed. In our study, SNP/mutation analysis was performed in the gene region responsible for the hydrolase activity of EPHX2, which encodes the soluble epoxide hydrolase enzyme. Material and Methods: The study consisted of two groups: a healthy group with 30 individuals and a T2DM patient group with 40 individuals. SNP/mutation analysis in the gene region responsible for the hydrolase activity of EPHX2 in both groups was performed by Sanger sequencing using appropriate primers. Result: A total of 12 mutations were detected in both groups as a result of Sanger sequencing. Two of the 12 detected mutations were missense mutations (p.Asn359Thr and p.Ser412Arg). It was determined that the pathogenic scores of these mutations were close to 1 for Poly-Phen2 and 0-100 for SNAP. In addition, two (c.1058+165C>T and c.1058+146G>A) SNPs were detected in the intron we observed in the T2DM group, which has not been detected and defined before in our study. Discussion: We believe that the mutations detected in our study, especially those that cause amino acid changes, may cause T2DM susceptibility in healthy individuals and progression of the disease pathogenesis in the T2DM group. We think that the detection of c.1058+165C>T and c.1058+146G>A mutations for the first time in our study will guide the next studies.Öğe Identification of potential microRNA markers related to Crimean-Congo hemorrhagic fever disease(Wiley, 2019) Arslan, Serdal; Engin, Aynur; Aydemir, Eylem Itir; Sahin, Nil Ozbilum; Bayyurt, Burcu; Sari, Ismail; Cosgun, YaseminCrimean-Congo hemorrhagic fever (CCHF) is a tick-borne disease caused by the arbovirus Crimean-Congo hemorrhagic fever virus (CCHFV). The CCHFV has a single-stranded RNA genome of negative sense. MicroRNAs (miRNAs) are key players in virus-host interactions and viral pathogenesis. We investigated the miRNA gene expression profiles in patients with CCHF using microarray for the first time in the world. Microarray analysis was performed using mirBase Ver 21 (Agilent Technologies, Santa Clara, CA). All statistical analyses were performed across the case-control, fatal-control, and fatal-nonfatal case groups using Genespring (Ver 3.0). Fifteen miRNAs were statistical significant in patients with CCHF compared with the controls (5 were upregulated, 10 were downregulated). Seventy-five and sixty-six miRNAs are in fatal compared with control and nonfatal case, respectively (fold change ([FC] >= 50) were statistically significant. In this study, the target genes of important miRNAs were identified and Gene Ontology analyses were performed across all groups. As a result of this study, we propose that the detection of miRNAs in patients with CCHF will allow the determination of therapeutic targets in diseases. CCHF is an important public health problem that can often be fatal. In this study, we investigated miRNA expression in case-control, fatal-control, and fatal-nonfatal case groups. Significant miRNAs associated with fatality were detected in CCHF. This study will serve as a source of data for the development of an antagomir-based therapy against CCHF using miRNAs in the future.Öğe IN VIVO HEPATOPROTECTIVE EFFECTS OF Hypericum crenulatum BOISS ON NIMESULIDE-INDUCED TOXICITY IN MICE(Parlar Scientific Publications (P S P), 2022) Ozkan, Oktay; Ozbey, Caner; Arat, Gonca Donmez; Kart, Asim; Sari, Ismail; Aydemir, IsilPlants of Hypericaceae family including many species have been used for the treatment of various dieases such as melancholia, abdominal pain, wound, tumor and oxidative stress in folk medicine. Hypericum crenulatum Boiss is a member of this family and is found throughout Bolkar mount from Meydan Yayla to San Tepe. It is an endemic herbaceous perennial plant. It is aimed to investigate in this study whether this plant specie have a hepatoprotective properties against nimesulide-induced liver injury in mice. According to the results of the study, methanolic extract of Hypericum crenulatum Boiss showed significant differences on serum ALP and liver histopathology. However, serum AST and ALT values, TAS, TOS and OSI were not altered. Hypericum crenulatum Boiss significant improvement Boiss extract at low dose provided a in nimesulide-induced acute liver toxicity. The data indicate that methanolic extract of Hypericum crenulatum Boiss is considered to be effective against liver toxicity.Öğe Serum Irisin Levels and Its Relationship with Spasticity Severity in Chronic Stroke Patients(Thieme Medical Publ Inc, 2022) Kaydok, Ercan; Sari, Ismail; Ersan, Serpil; Kucuk, Esin BenliObjective Irisin is a myokine released from muscles by exercise and it has been shown to be a prognostic indicator in acute stroke patients. However, irisin's relationship with the chronic phase of stroke and spasticity has not been studied yet. We aimed to determine the serum level of irisin to examine its relationship with the functional status and severity of spasticity in patients with chronic stroke, and to compare these with healthy controls. Materials and Methods A total of 70 (35 chronic stroke and 35 control patients) patients were included in the study. The blood serum irisin levels of the patients and the controls were evaluated using enzyme-linked immunosorbent assay method, their functional status was evaluated with the modified Rankin scale (mRS), and spasticity severity using the modified Ashworth scale (MAS). Results The mean serum irisin levels of the stroke and the control groups were 6.20 +/- 2.2 and 5.45 +/- 2.3, respectively, and there was no statistically significant difference ( p > 0.05). No significant correlation was found between the serum level of irisin and the severity of spasticity and functional status, assessed by the mRS in stroke patients. Conclusion These results showed that irisin levels in chronic stroke patients were similar to controls, and there was no relationship between the severity of spasticity and functional status and irisin level.Öğe Serum malondialdehyde, monocyte chemoattractant protein-1, and vitamin C levels in wet type age-related macular degeneration patients(Sage Publications Ltd, 2020) Zor, Ramazan Kursad; Ersan, Serpil; Kucuk, Erkut; Yildirim, Gamze; Sari, IsmailPurpose: The purpose of this study was to investigate the serum levels of malondialdehyde (MDA) which is a marker of oxidative stress, monocyte chemoattractant protein-1 (MCP-1) which has an important role in inflammation, and vitamin C which has antioxidant properties in patients with wet age-related macular degeneration (wAMD). Methods: Thirty patients with wAMD were included in the study and serum levels of MDA, MCP-1, and vitamin C were compared with healthy participants (n = 30). Serum vitamin C and MDA levels were measured using a spectrophotometric method. Serum MCP-1 levels were determined by the ELISA method. Results: MCP-1 and MDA levels were higher in patients with wAMD compared with the control group (p < 0.05). Serum vitamin C levels were lower in patients with wAMD compared with the control group (p < 0.05). Conclusions: The increase in the MCP-1 levels in patients with wAMD may be associated with increased inflammation in wAMD. Decreased serum vitamin C and elevated MDA levels in patients with wAMD suggest increased oxidative stress in wAMD patients. These results indicate that the increased oxidative stress and inflammation can play a role in the pathogenesis of wAMD.Öğe Shrinking Lung Syndrome in Primary Sjogren Syndrome(Galenos Publ House, 2020) Uslu, Sadettin; Avsar, Aydan Koken; Erez, Yesim; Sari, Ismail[Abstract Not Available]Öğe Significant association between MDM2 T309G polymorphism and colorectal cancer(Imprimatur Publications, 2019) Atabey, Mustafa; Tas, Ayca; Sari, Ismail; Bostanci, Meric Emre; Topcu, Omer; Silig, YavuzPurpose: The Murine double minute 2 (MDM2) gene plays a crucial role in regulating and suppressing the function of apoptotic pathway. We investigated the relationship between MDM2 gene SNP309 (T309G) (rs2279744) polymorphism and colorectal cancer (CRC) in a Turkish population. Methods: The polymorphism T309G (rs2279744) in the MDM2 gene was studied in patients with colorectal cancer (n=135) and healthy control subjects (n=145) using the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method The findings were evaluated using logistic regression and x(2) tests. Results: When CRC cases and controls were evaluated based on different habits and family cancer histories, a statistically significant relationship was found between CRC and alcohol consumption (x(2) =4.07, p=0.044). Cancer cases and controls had statistically significant different family histories of cancer (x(2) =6.82, p =0.009). There was also significant difference in TG genotype distribution in the MDM2 T309G polymorphism between those with and without cancer (OR=1.98, 95% CI =1.98-3.91, x(2) =4.00, p =0.045). Conclusions: The SNP309 polymorphism of the MDM2 gene is associated with increased CRC risk in the Turkish population.Öğe The effect of acute step-aerobic exercise on adiponectin and leptin levels in premenopausal women(Edizioni Minerva Medica, 2021) Sari, Ismail; Habipoglu, Sedef; Seydel, G. Seyda; Ersan, Serpil; Gunturk, InayetBACKGROUND: It is well known that the prevalence of obesity, cardiovascular disease and metabolic syndrome is increasing during the transition to menopausal stages. The aim of this study was to investigate the effects of acute step-aerobic exercise on levels of leptin and adiponectin, which are adipose tissue-derived adipocytokines, associated with obesity, hypertension and other diseases in sedentary premenopausal women. METHODS: Twenty-four sedentary premenopausal volunteer women between the ages of 40-45 (premenopause group) and 24 healthy adults between the ages of 21-39 (control group) were enrolled in this study. Moderate intensity step-aerobic exercise (50-60% of HRmax) was applied for 60 min to the premenopausal subjects. Venous blood samples were obtained before and after the exercise. Adiponectin and leptin levels were determined using the ELISA method. RESULTS: No statistically significant difference was found in terms of BMI values, and leptin levels between the premenopause and control group, while differences of adiponectin were statistically significant. Plasma leptin levels of the premenopausal women decreased whereas adiponectin levels increased meaningfully after the acute aerobic exercise (P<0.05). When the premenopausal women were grouped according to BMI, there were no significant differences between BMI <= 30 and >30 groups in terms of leptin and adiponectin levels measured before and after exercise. CONCLUSIONS: Our results may suggest that acute step aerobic exercise in premenopausal women alters leptin and adiponectin levels in favor of the organism. However, further studies are needed.
