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Öğe Contributions to the Karyology and Distribution Areas of Cytotypes of Nannospalax leucodon (Rodentia: Spalacidae) in Western Anatolia(INST ZOOLOGY, BAS, 2010) Kankilic, Teoman; Kankilic, Tolga; Seker, Perincek Seckin; Colak, Reyhan; Selvi, Engin; Colak, ErcuementMole rats of the genus Nannospalax having high karyotypic variability (chromosome numbers ranging from 2n = 36 to 2n = 62) are dominant mammals occupying the subterranean niche in Anatolia. Respective distributional ranges of karyotypic forms within this taxon remain uncertain due to insufficient data. In the present study, karyological analyses of 36 specimens belonging to N. nehringi were examined from 10 sampling sites of Western Turkey. During the study, five karyotypic forms were recorded (2n = 36 NF = 68 from Aydin, 2n = 40 NF = 72 from Isparta, 2n = 56 NF = 72 from Usak, 2n = 60 NF = 78 from Isparta and 2n = 60 NF = 84 from Denizli and Burdur). When distribution areas of karyotipic forms are considered, these karyotypes are new records for this taxon in Turkey. In addition, this study aims to determine the most likely distribution areas of the four chromosome forms (2n = 36, 40, 56, 60) in Turkey paying attention on previously verified localities data.Öğe G-banded Karyotypes of Some Species in Gliridae (Mammalia: Rodentia) from Turkey(Adiyaman University, 2021) Kankiliç, Teoman; Şeker, Perinçek Seçkinozan; Selvi, Engin; Özkan, Beytullah; Yiğit, Nuri; Çolak, ErcümentThe results of a cytogenetic study on some representatives of Gliridae from Turkey were introduced. The G-, C-, and AgNOR banded karyotypes of Muscardinus avellanarius abanticus from Abant (Bolu), and the G-banded karyotype of Myomimus roachi from Thrace were presented for the first time. Additionally, the G-banded karyotypes of Dryomys nitedula, and Myoxus glis were analysed. Because of not including a secondary constriction and the smallest chromosome being metacentric instead of acrocentric in the autosomal set, the karyotype of M. a. abanticus was different from that of M. a. trapezius. With the comparison of obtained G-banded patterns belonging to the other Glirid species, it was detected that obtained karyotypes displayed consistency at a great extent with the previously determined karyotypes. © 2021, Adiyaman University. All rights reserved.Öğe Geographical Distribution Pattern of Mitochondrial DNA Cytochrome b Diversity in Populations of Arvicola amphibius (Linnaeus, 1758) (Mammalia: Rodentia) in Turkey as Determined by PCR-RFLP(INST ZOOLOGY, BAS, 2018) Seker, Perincek Seckinozan; Selvi, Engin; Kankilic, Teoman; Colak, ErcumentArvicola amphibius (Linnaeus, 1758) is a semiaquatic rodent living in and around wetland habitats covered by rich vegetation. It has a broad distribution in the Palearctic and three subspecies live in Turkey. In order to identify the geographical distribution of mitochondrial DNA cytochrome b diversity in A. amphibius populations in Turkey, the restriction fragment length polymorphism method was applied. The digestion patterns of four restriction enzymes (Alu I, Rsa I, Sau3A I and EcoR V) in 100 samples of A. amphibius from 20 localities in Turkey brought out four mtDNA lineages [Thrace (THR), central Anatolia (CA), southern Anatolia (SA) and eastern Anatolia (EA)], each having high genetic diversity. In contrast to the three lineages in THR, CA and SA, respectively, corresponding to the known subspecies A. a. cernjavskii, A. a. persicus and A. a. hintoni, separate populations constituting the fourth A. amphibius lineage in EA were determined. The results proved that the variable topography and geomorphological conglomeration process of Anatolia lead to an allopatric differentiation and had a large impact on the appearance and shaping of intraspecific genetic variations in water voles. Moreover, the existing high genetic variability in Turkish water vole populations propounds the importance of Anatolia as a potential refuge in the Pleistocene.Öğe NUCLEAR AND ORGANELLE GENES BASED PHYLOGENY OF DRYOMYS (GLIRIDAE, RODENTIA, MAMMALIA) FROM TURKEY(Hungarian Natural History Museum, 2019) Kankilic, Teoman; Seker, Perincek Seckinozan; Aydin, Burcu; Altunbas, Derya; Selvi, Engin; Yigit, Nuri; Colak, ErcumentMolecular phylogeny, phylogeography and genetic structure of the genus Dryomys Thomas, 1906 from Turkey were identified by using partial sequences of beta-fibrinogen intron 7 and mitochondrially encoded 12S ribosomal RNA genes and also combined data of two genes. Within Dryomys nitedula species, both, nuclear and mitochondrial genes coherently separated the Thrace lineage from the other lineages in Anatolia. Contrary to this, complex and incomprehensible phylogenies were recovered for Anatolian populations of this species. The analysis of the combined data of these two genes resolved mentioned complexity and incongruity and made phylogeny compatible with the results of past studies for the relative position of the Anatolian lineages. Thus, the presence of four different lineages (one in Thrace and three in Anatolia) within D. nitedula in the localities exemplified across Turkey was confirmed. Genetic differentiation (K2P distances) between the lineages were moderate at the level of intraspecific diversity. In addition to this, genetic distance (K2P = 5.5%) determined between D. nitedula and D. laniger conformed the distance suggested for the separate species of mammals. Evolutionary divergence time estimations demonstrated that the probable divergence between D. laniger and D. nitedula and among its detected lineages started in the border of Late Miocene and Pliocene (5.3 Mya) and lasted to the beginning of the Calabrian Stage of Pleistocene (1.8 Mya) in line with the previous results obtained from fossil and molecular data.Öğe Phylogenetic Inference from 16S rRNA Gene Sequencing in Chromosome Races of the Genus Nannospalax Species (Rodentia: Spalacidae)(Pleiades Publishing Inc, 2024) Kankilic, Teoman; Celikbilek, Habibe Didem; Kankilic, Tolga; Seker, Perincek Seckinozan; Selvi, Engin; Civelek, IlkayIn this study, we aimed to develop a species-level phylogeny for the Nannospalax genus, identify cryptic species that are difficult to differentiate using standard methods, and further evaluate the relationships across chromosomal forms of several species. 16S rRNA gene sequences from 81 Turkish regions were evaluated and statistical analyzes were conducted. Results are as follows; (i) Nannospalax cilicicus was found to be monophyletic and sister in relation to Nannospalax xanthodon; (ii) We suggested for the first time that the Central-Anatolian populations should be classified under the species name Nannospalax cilicicus (stat. n.); (iii) the unidentified 2n = 52 cytotypes from Bolu and Nannospalax leucodon cytotypes were reciprocally monophyletic, these data support the notion that the 2n = 52 cytotypes from Bolu belong to an unidentified taxon; (iv) study also offered evidence for the monophyly of N. tuncelicus for the first time; (v) Nannospalax xanthodon (2n = 36, 38, and 40) and Nannospalax nehringi composed several species-specific clades, which form polytomy structure, so relationships between these species are still unclear.Öğe Variation in the Conventional and Banded Karyotypes among Populations of Arvicola amphibius (L., 1758) (Mammalia: Rodentia) from Turkey(INST ZOOLOGY, BAS, 2018) Seker, Perincek Seckinozan; Arslan, Atilla; Selvi, Engin; Kankilic, Teoman; Zima, JanChromosomal characteristics of water voles (Arvicola amphibius) were studied in ten populations including 33 samples from Anatolia, Turkey. The C-banding pattern and NORs distribution were analysed in four samples from Eastern Anatolia. The conventionally stained karyotypes showed the standard complement of the species (2n = 36, NFa = 60-62, NF = 64-66). Variation in the number of autosomal arms originated from the alternative presence of a subtelocentric or an acrocentric autosomal pair. C-banding provided further support for differentiation of the amount and distribution of C-heterochromatin between populations from Central Europe and Asia Minor. Chromosomal variation among Turkish populations was manifested by the number of autosomal arms, positive C-bands and NOR-carrying autosomes.