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    Evaluation of some horticulturally important morphological traits of Turkish wild Vaccinium species
    (International Society for Horticultural Science, 2019) Bozdag, M.M.; Sultana, N.; Serçe, S.
    Turkey has rich gene pool of several economically important Vaccinium species which are biologically closely related with the cultivated blueberry species. Although fruit of those Vaccinium species have been collected from the nature by local inhabitants for millennium, they are not commercially cultivated. A study was carried out leading to the collection and characterization of morphological traits of those wild Vaccinium species having potential horticultural importance. Five morphologically different populations of Vaccinium wild species were collected from different habitat with diverse altitude during the land expedition of Eastern Black sea region of Turkey. After extensive literature search, focusing on their morphological characters, these Vaccinium populations were identified as Whortleberry (V. acrostaphylos), billberry (V. myrtillus) and bog bilberry (V. uliginosum). Among the five populations three were highbush type V. acrostaphylos and two lowbush type species identified as V. myrtillus and V. uliginosum. Twelve horticulturally important morphological traits like leaf weight, petiole length, leaf width, leaf length, leaf color (upper side), leaf color (lower side), fruit weight, fruit width, fruit length, fruit crown width, seed number and fruit color have been recorded and analyzed in details. Significant differences observed among the populations and species level regarding the analyzed traits. It is evident that our initial result could be highly valuable for further characterization and improvement of wild or cultivated Vaccinium species in Turkey. © 2019 International Society for Horticultural Science. All rights reserved.
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    GENOTYPIC IDENTIFICATION AND CHARACTERISATION OF PROBIOTIC PROPERTIES OF LACTIC ACID BACTERIA FROM NIGDE CHEESE
    (SCIBULCOM LTD, 2016) Ilk, S.; Canpolat, E.; Ozgen, M.; Saglam, N.; Sultana, N.
    Probiotics are live microorganisms having profound health benefit on its host and among them lactic acid bacteria (LAB) are common group found in human food. As this is a very vast group of microorganisms having different strains, therefore genotypic identification has been performed by primers that are specific for 16s ribosomal DNA gene on a total of 24 exopolysaccharides (EPS) producing LAB: those are Lactobacillus, Enterococcus and Pediococcus isolated from Nigde cheese of Turkey. Subsequently, characterization of probiotic properties of LAB was studied by final pH, acidification, and amount of EPS concentration and aggregation properties. EPS productions by the strains were found between 54.1-93.6 mg/l. The highest EPS production was detected in the Pediococcus pentosaceus EC18 and the lowest EPS were estimated in the Enterococcus faecium EC26. All LAB strains showed auto- and coaggregation ability with Escherichia coli (E. coli) ATCC 25923 and Salmonella enterica serotype typhmurium (S. typhmurium) SL 1344. Results showed that the aggregation property is specific for each strain and dependent on EPS production. Our results indicate that the ability to autoaggregate, together with exopolysaccharide and final pH properties and coaggregation abilities with E. coli and S. typhmurium strains, can be used for preliminary screening in order to identify potentially probiotic bacteria suitable for human.
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    Genotypic identification and characterisation of probiotic properties of lactic acid bacteria from nigde cheese
    (Scibulcom Ltd., 2016) Ilk, S.; Canpolat, E.; Ozgen, M.; Saglam, N.; Sultana, N.
    Probiotics are live microorganisms having profound health benefit on its host and among them lactic acid bacteria (LAB) are common group found in human food. As this is a very vast group of microorganisms having different strains, therefore genotypic identification has been performed by primers that are specific for 16s ribosomal DNA gene on a total of 24 exopolysaccharides (EPS) producing LAB: those are Lactobacillus, Enterococcus and Pediococcus isolated from Nigde cheese of Turkey. Subsequently, characterization of probiotic properties of LAB was studied by final pH, acidification, and amount of EPS concentration and aggregation properties. EPS productions by the strains were found between 54.1–93.6 mg/l. The highest EPS production was detected in the Pediococcus pentosaceus EC18 and the lowest EPS were estimated in the Enterococcus faecium EC26. All LAB strains showed auto-and coaggregation ability with Escherichia coli (E. coli) ATCC 25923 and Salmonella enterica serotype typhmurium (S. typhmurium) SL 1344. Results showed that the aggregation property is specific for each strain and dependent on EPS production. Our results indicate that the ability to autoaggregate, together with exopolysaccharide and final pH properties and coaggregation abilities with E. coli and S. typhmurium strains, can be used for preliminary screening in order to identify potentially probiotic bacteria suitable for human. © 2016, Scibulcom Ltd. All rights reserved.