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Influences of basal media, growth regulators, explant type and photoperiod on callus competency and pigmentation of Alkanna orientalis L.
(2021) Yaman, Cennet; Bakhsh, Allah; Uranbey, Serkan
The goal of this research was in-vitro callus induction and red pigment production of Alkanna orientalis as a medicinal herb which belongs to Boraginaceae family containing valuable naphthoquinone derivates. The two different explants (leaf and leaf base) were subjected to abiotic factors such as different nutrient media (MS, B5 and M9), light and plant growth regulators (IAA and IBA). High frequency reproducible, prolific and compact calli formation was obtained from MS and B5 media supplemented with IAA, whereas high pigmentation was found in leaf base explants on M9 medium. Leaf base explant and dark conditions were found more effective for both callus formation and pigment production. The pigmentation at IBA was more than IAA, and the maximum level of pigmentation was observed on M9 medium with a combination of 1.0 mg/l IBA under dark. The factors that may be the most influential in the production of callus and red color pigment from A. orientalis have been determined. A. orientalis may be considered to be alternative plants for the A/S (alkannin/shikonin) production in vitro.