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Öğe Are follistatin-like protein 1 and follistatin-like protein 3 associated with inflammatory processes in patients with familial Mediterranean fever?(Kare Publ, 2023) Kaplan, Huseyin; Calis, Mustafa; Yazici, Cevat; Gunturk, Inayet; Cuce, Isa; Senel, Abdurrahman SonerOBJECTIVE: Follistatin-like protein 1 (FSTL-1) and follistatin-like protein 3 (FSTL-3) are glycoproteins whose associations with inflammatory cytokines were reported in previous studies. However, it is not yet known whether they have an effect on the pathogenesis of familial Mediterranean fever (FMF). We aimed to detect the FSTL-1 and FSTL-3 levels and to determine their relationship to the attack status and mutation types in patients with FMF. METHODS: Fifty-six FMF patients and 22 healthy controls (HCs) were included in the study. Serum FSTL-1 and FSTL-3 levels were measured with the enzyme-linked immunosorbent assay method from collected serum samples. In addition, the MEditerranean FeVer (MEFV) gene mutation types of the patients were noted. RESULTS: Serum FSTL-1 levels were significantly higher in FMF patients than in HCs (p=0.005). However, there was no significant difference in FSTL-1 levels between patients in the attack period (n=26) and in the attack-free period (n=30). FSTL-3 levels were similar between FMF patients and HCs or patients in the attack period and in the attack-free period. Furthermore, the MEFV mutation type and attack status had no significant effect on FSTL-1 and FSTL-3 levels (p>0.05). CONCLUSION: Our results suggest that FSTL-1 may be associated with the pathogenesis of FMF, rather than FSTL-3. However, neither serum FSTL-1 nor FSTL-3 seems to be good markers to reflect inflammatory activity.Öğe Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney(Cukurova Univ, Fac Medicine, 2022) Gunturk, Inayet; Seydel, G. Seyda; Dagli, Fatma; Yay, Arzu; Yazici, Cevat; Kose, KaderPurpose: Cisplatin is one of the most potent and widely used chemotherapeutic agents for the treatment of a wide variety of solid organ cancers. However, due to various side-effects such as nephrotoxicity, its therapeutic applications are limited. In the current study, it was aimed to investigate the effects of N-acetylcysteine (NAC), which is an effective antioxidant and anti-inflammatory agent, on cisplatin-induced apoptosis in rat kidneys. Materials and Methods: Twentyfour male Wistar rats were separated into 4 equal groups: Control, NAC-250, cisplatin (CP), and CP+NAC groups. Rats in the experimental groups were treated with intraperitoneally (i.p.) single-dose cisplatin (10 mg/kg) and NAC (i.p., 250 mg/kg) for 3 days. Results: At the end of the experiment, nephrotoxicity was confirmed by blood urea nitrogen and creatinine levels, and the apoptotic changes were demonstrated by TdT-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) and caspase-3 levels in rat kidneys. The number of TUNEL-positive cells and caspase-3 levels were significantly increased by cisplatin. Treating the rats with NAC significantly decreased TUNEL-positive cells and caspase-3 levels. Conclusion: These data suggest that apoptotic cell death is involved in the pathogenesis of cisplatin-induced nephrotoxicity, and that the inhibition of apoptosis plays a central role in the beneficial effects of NAC.Öğe Evaluation of physical and vital signs and the effect of carnosine in experimental hyperthyroidism(Cukurova Univ, Fac Medicine, 2022) Dagli, Fatma; Gunturk, Inayet; Seydel, Gonul Seyda; Yazici, CevatPurpose: This study aims to investigate the effects of experimental hyperthyroidism and carnosine which is known to have antioxidant properties on physical and vital findings in rats, and to determine the relationship between these parameters and free T3 (FT3) levels. Materials and Methods: Rats were analyzed in 7 groups (each containing 12 animals); control (CONT), hyperthyroidism-1 (T:10-day L-thyroxine (L-T4) administration), hyperthyroidism-2 (T-T: 20-day L-T4 administration), Carnosine (10 day carnosine administration), Hyperthyroidism-1 + Carnosine (T-C), Hyperthyroidism-2 + Carnosine (T-TC), and Carnosine + Hyperthyroidism-1 (C-T). In order to create a hyperthyroidism model, L-thyroxine (L-T4) doses of 300 mu g/kg rat weight/day and carnosine doses of 300 mu g/kg rat weight/ day were intraperitoneally (ip) administered to the rats. Results: After 10 and 20 days of thyroxine administration, FT3 levels (T:3.64 +/- 0.51pg/mL, T-T: 4.06 +/- 0.91pg/mL) and body temperature (T:37.1 +/- 0.3oC, T-T: 37.6 +/- 0.3oC), significantly increased while body weight decreased (T:240.7 +/- 22.0g, T-T:263 +/- 0.28.7g). Carnosine administration only prevented the increase of FT3 levels, but had no effect on other parameters. Conclusion: The increased FT3 levels observed with LT4 administration were consistent with the physical and vital findings, but carnosine administration did not reflect the expected effects on the physical findings observed in the hyperthyroid condition.Öğe Evaluation of the relationship between disease severity and the leptin, adiponectin, and chemerin levels in overweight and obese COVID-19 patients(Bayrakol Medical Publisher, 2024) Seydel, Gonul Seyda; Gunturk, Inayet; Yazici, Cevat; Topaloglu, Ulas Serkan; Eren, Esma Eryilmaz; Yuksel, Recep CivanAim: Overweight and obesity are substantial risk factors in the severity of COVID-19 disease. This study aimed to assess the relationship between the disease severity and leptin, adiponectin, and chemerin levels in overweight and obese COVID-19 patients. Material and Methods: The study involved 60 COVID-19 patients (patient group) and 30 healthy controls with BMI >= 25. The patient group was split into two subgroups based on disease severity (30 mild/moderate and 30 severe/critical patients). The levels of leptin, adiponectin, and chemerin in plasma were determined using the ELISA technique. Results: Our study revealed that leptin levels were considerably increased in both groups of COVID-19 patients compared to the healthy controls, while chemerin levels were decreased. In adiponectin levels, there was no statistically significant difference between the groups. Discussion: Plasma leptin and chemerin levels are associated with the progression and/or severity of disease in overweight and obese COVID-19 patients.Öğe Examination of the relationship between variants in the gene region encoding soluble epoxy hydrolase enzyme hydrolytic activity and type 2 diabetes(Bayrakol Medical Publisher, 2024) Ozmen, Esma; Ayan, Durmus; Onder, Cagatay Emir; Akin, Dilara Fatma; Kose, Burcu; Sari, Ismail; Yazici, CevatAim: Epoxyeicosanoids function as signal mediators in critical biological processes such as platelet aggregation, vasodilation, and anti -inflammation. With all these properties, Epoxyeicosanoids have been associated with many diseases. Metabolism of epoxyeicosanoids is carried out by soluble epoxide hydrolase enzymes, and as a result dihydroxyeicosatrienoic acids, which is a less active form than epoxyeicosanoids, are formed. In our study, SNP/mutation analysis was performed in the gene region responsible for the hydrolase activity of EPHX2, which encodes the soluble epoxide hydrolase enzyme. Material and Methods: The study consisted of two groups: a healthy group with 30 individuals and a T2DM patient group with 40 individuals. SNP/mutation analysis in the gene region responsible for the hydrolase activity of EPHX2 in both groups was performed by Sanger sequencing using appropriate primers. Result: A total of 12 mutations were detected in both groups as a result of Sanger sequencing. Two of the 12 detected mutations were missense mutations (p.Asn359Thr and p.Ser412Arg). It was determined that the pathogenic scores of these mutations were close to 1 for Poly-Phen2 and 0-100 for SNAP. In addition, two (c.1058+165C>T and c.1058+146G>A) SNPs were detected in the intron we observed in the T2DM group, which has not been detected and defined before in our study. Discussion: We believe that the mutations detected in our study, especially those that cause amino acid changes, may cause T2DM susceptibility in healthy individuals and progression of the disease pathogenesis in the T2DM group. We think that the detection of c.1058+165C>T and c.1058+146G>A mutations for the first time in our study will guide the next studies.Öğe Increased hip circumference in individuals with metabolic syndrome affects serum nesfatin-1 levels(Bmj Publishing Group, 2020) Tekin, Tuba; Cicek, Betul; Konyaligil, Nurefsan; Gunturk, Inayet; Yazici, Cevat; Karaca, Zuleyha; Unlusavuran, MeltemBackground This case-control study was conducted to investigate the relationship between serum nesfatin-1 levels and nutritional status and blood parameters in patients diagnosed with metabolic syndrome. Methods Thirty patients (case) diagnosed with metabolic syndrome according to National Cholesterol Education Program-Adult Treatment Panel III criteria were included. Thirty healthy subjects (control) matched with patients with metabolic syndrome in terms of age, gender and body mass index were included. Three-day food consumption records were obtained. Anthropometric indices were measured and body composition was determined by bioelectrical impedance method. Biochemical parameters and serum nesfatin-1 levels were measured after 8 hours of fasting. Results Serum nesfatin-1 levels were 0.245 +/- 0.272 ng/mL in the case group and 0.528 +/- 0.987 ng/mL in the control group (p>0.05). There was a positive significant correlation between serum nesfatin-1 levels and body weight, waist and hip circumferences in the case group (p<0.05). Each unit increase in hip circumference measurement affects the levels of nesfatin by 0.014 times. In the control group, there was a positive significant correlation between body weight and serum nesfatin-1 levels (p<0.05). A significant correlation was detected between HbA1c and serum nesfatin-1 levels in the case group (p<0.05). A significant relationship was detected between dietary fibre intake and the serum nesfatin-1 levels in the case group (p<0.05). Conclusions Anthropometric indices and blood parameters were correlated with serum nesfatin-1 levels in patients with metabolic syndrome. More clinical trials may be performed to establish the relationship between serum nesfatin-1 levels and nutritional status.Öğe IS THER ANY ROLE OF THE URINE DICKOPPF-3/CREATININE RATIO IN EARLY DETECTION OF ACUTE KIDNEY INJURY IN PEDIATRIC INTENSIVE CARE UNIT?(Springer, 2022) Gokceli, Sefa Armagan; Gunay, Neslihan; Gunturk, Inayet; Dundar, Mehmet Akif; Akyildiz, Basak Nur; Yazici, Cevat; Yel, Sibel[Abstract Not Available]Öğe Response to chronic sustained hypoxia: increased cytosolic gelsolin and decreased plasma gelsolin levels(Springer, 2024) Gunturk, Inayet; Kuloglu, Nurhan; Seydel, Gonul Seyda; Yazici, Cevat; Basaran, Kemal Erdem; Yakan, Birkan; Karabulut, DeryaAn actin binding protein, gelsolin (GSN) has two isoforms, plasma (pGSN) and cytosolic (cGSN). Changes in pGSN and/or cGSN levels have been shown to be associated with the pathogenesis of several diseases. The aim of this study was to evaluate changes in intracellular and extracellular GSNlevels with HIF-1 in animals exposed to chronic sustained hypoxia (CSH), in addition to apoptosis and the cellular redox status. The rats in the Sham group were exposed to 21% O-2, and the rats in the hypoxia groups were exposed to 13 and 10% O-2, respectively. Plasma pGSN, HIF-1 alpha, Total Antioxidant Status (TAS) and Total Oxidant Status (TOS), and lung tissue pGSN, HIF-1 alpha, TAS, TOS, GSN levels, and apoptotic cell numbers were measured. HIF-1 alpha levels were found to increase significantly in the tissue, especially in the group with severe hypoxia, both in biochemical and histological examinations. pGSN levels were also significantly decreased in both plasma and tissue. Significant increases in tissue were observed in cGSN. It was observed that while the antioxidant activity was dominant in the tissue, the oxidant activity was dominant in the plasma. In particular, the response to hypoxia regulated by HIF-1 is very important for cellular survival. The results of this study showed that the increase in cGSN and TAS levels in the lung tissue together with HIF-1 alpha can be considered as the activation of mechanisms for cellular protection.Öğe Serum visfatin levels are positively correlated with dietary carbohydrate and polyunsaturated fatty acid intakes in type 2 diabetes mellitus patients(Elsevier Science Inc, 2023) Ozturk, Nurefsan Konyaligil; Cicek, Betul; Tekin, Tuba; Gunturk, Inayet; Yazici, Cevat; Karaca, Zuleyha; Sonmez, MeltemAims: To investigate the relationship between dietary intake and biochemical parameters and anthropometric measurements and serum visfatin concentrations. Study design: A case-control study. Methods: The study was carried out in 30 type 2 diabetes mellitus (T2DM) and 30 sex, age and body mass index (BMI) matches healthy control subjects. Biochemical parameters (glycemic and lipid profile, insulin resistance), anthropometric measurements (weight and bioimpedance) and dietary intake evaluation were obtained. Visfatin was assayed with ELISA method. Results: The mean BMI of the case group was 31.36 +/- 4.37 kg/m(2) and 29.80 +/- 3.53 kg/m(2) in the control group (p = 0.134). The results revealed a significant increase in the weight, waist circumference, waist/hip ratio, visceral fat ratio, fasting glucose level, HbA1c and fasting insulin as well as in insulin resistance (HOMA-IR) among T2DM patients when compared with controls (p < 0.05). Serum visfatin levels were higher in the subjects with T2DM than healthy control subjects (p < 0.05). There was no significant correlation between visfatin levels and biochemical parameters and anthropometric measurements in patients with T2DM. Serum visfatin level was positively correlated with carbohydrate (CHO) and polyunsaturated fatty acid (PUFA) in T2DM patients (r = 0.406, p = 0.026; r = 0.404, p = 0.027, respectively). Conclusion: T2DM patients compares with healthy control group increased serum visfatin levels. PUFA and CHO intake was found to be positively associated with visfatin levels.Öğe The effect of N-acetylcysteine on inflammation and oxidative stress in cisplatin-induced nephrotoxicity: a rat model(Tubitak Scientific & Technological Research Council Turkey, 2019) Gunturk, Inayet; Yazici, Cevat; Kose, Kader; Dagli, Fatma; Yucel, Bilal; Yay, ArzuBackground/Aim: Cisplatin is a highly effective chemotherapeutic agent used in the treatment of solid organ cancers. Besides its chemotherapeutic effectiveness, cisplatin administration is associated with numerous side effects. Of those, the most clinically significant and common effect is nephrotoxicity. Recent studies reported that oxidative stress and inflammation are probably the most important mechanisms that contribute to the nephrotoxicity. N-acetylcysteine (NAC) is an antioxidant and antiinflammatory agent. In the present study, the effects of NAC on cisplatin-induced nephrotoxicity were investigated. Materials and methods: Rats were divided into four groups each including eight rats: CONT, NAC-250, CP, and CP+NAC. Rats in experimental groups were treated intraperitoneally (i.p.) with a single dose of cisplatin (10 mg/kg body weight) and i.p. with NAC (250 mg/kg body weight) for three consecutive days. Nephrotoxicity was determined by plasma BUN and creatinine levels. In tissue samples, myeloperoxidase (MPO), nuclear factor-kappa B (NF-kB), high mobility group box-1 (HMG B-1), total oxidant status (TOS), and total antioxidant status (TAS) levels were measured. Kidneys were analyzed histopathologically as well. Results: It was revealed that cisplatin was not effective on MPO, HMGB-1 and NF-kB levels but did increase TOS levels and decrease TAS levels in tissue samples. Interestingly, NAC elevated MPO and HMGB-1 levels significantly. Nevertheless, NAC ameliorated histological and functional changes in kidney tissues. Conclusion: It is suggested that inflammation has a limited effect on cisplatin nephrotoxicity in this experimental design, and, as reflected by decreased BUN and creatinine levels, NAC can be used as an additional therapeutic agent in standard cisplatin treatment protocols.Öğe The Relationship Between Plasma Gelsolin Levels and Myeloperoxidase in Patients Undergoing Hemodialysis: A Prospective, Observational, Controlled Study(Erciyes Univ Sch Medicine, 2024) Tural, Haci Huseyin; Kose, Kader; Gunturk, Inayet; Kocyigit, Ismail; Yazici, CevatObjective: Given the association of inflammatory conditions with the development of comorbidities, particularly cardiovascular diseases, it is crucial to monitor inflammation in hemodialysis patients. This study aimed to evaluate plasma gelsolin and myeloperoxidase (MPO) levels before and after dialysis sessions and to assess their relationship with inflammation. Materials and Methods: The study included 16 healthy volunteers and 30 patients receiving regular hemodialysis treatment. Along with routine biochemical analyses, plasma gelsolin and MPO levels were measured in blood samples taken from the study group before and after the sessions. Results: Plasma gelsolin levels were found to be statistically higher both before and after dialysis compared to the control group (p=0.000); however, there was no significant change during the session (p=0.094). Conversely, plasma MPO activity, which was significantly higher before dialysis, increased at the end of the session (p=0.000). Conclusion: It can be concluded that elevated levels of gelsolin are associated with a chronic inflammatory response, as indicated by high -sensitivity C -reactive protein (hsCRP) and MPO levels. Consequently, gelsolin could be considered a supportive treatment strategy for these patients.Öğe The Systemic Immune-Inflammation Index Predicts In-Hospital Mortality in Patients Who Underwent On-Pump Cardiac Surgery(Arquivos Brasileiros Cardiologia, 2024) Gunturk, Inayet; Ozmen, Rifat; Ozocak, Okan; Gunturk, Ertugrul Emre; Dagli, Fatma; Yazici, CevatBackground: Systemic immune-inflammation index (SII), a new inflammatory index calculated using platelet, neutrophil, and lymphocyte counts, has been demonstrated to be an independent risk factor for the identification of high-risk coronary artery disease in patients undergoing percutaneous coronary intervention and cardiovascular surgery with cardiopulmonary bypass (CPB). The relationship between SII and CPB-related mortality rates remains unclear. Objective: This research was designed to investigate the use of SII to predict in-hospital mortality in patients undergoing cardiac surgery with CPB. Methods: Four hundred eighty patients who underwent a cardiac procedure involving CPB over 3 years, were obtained from the hospital's database. The demographic data, comorbidities, hematological and biochemical profiles, and operative data of the groups were compared. Multiple logistic regression analyses were done to determine independent predictors of mortality. Prognostic factors were assessed by multivariate analysis, and the predictive values of SII, neutrophil-lymphocyte ratio (NLR), and platelet-lymphocyte ratio (PLR) for mortality were compared. A p-value <0.05 was considered significant. Results: Of 480 patients, 78 developed in-hospital mortality after cardiac surgery. SII was an independent predictor of in-hospital mortality (Odds ratio: 1.003, 95% confidence interval: 1.001-1.005, p<0.001). The cut-off value of the SII was >811.93 with 65% sensitivity and 65% specificity (area under the curve: 0.690). The predictive values of SII, PLR, and NLR were close to each other. Conclusion: High preoperative SII scores can be used for early determination of appropriate treatments, which may improve surgical outcomes of cardiac surgery in the future.Öğe URINE DKK3 AND CHRONIC KIDNEY DISEASE PROGRESSION IN CHILDREN WITH CAKUT, IS IT A RELIABLE BIOMARKER?(Springer, 2022) PinarbaSi, Ayse Seda; GUnay, NeslIhan; GUntUrk, Inayet; Keti, Didem Barlak; Yel, Sibel; Ulueren, Sekure Rabia; Yazici, Cevat[Abstract Not Available]
