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    Determination of Subtypes, Serogroups, And Serotypes, Virulence, and/ or Toxigenic Properties of Escherichia coli Isolated From Cattle, Sheep, and Goat Feces by Multiplex PCR
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2024) Kizil, Sibel; Aydin, Fatma Esin; Onel, Aziz Utku; Yildirim, Murat; Gueneri, Cansu Onlen; Cecen, Efsun Melike
    In the study, rectal swabs taken from 300 ruminant animals including cattle (100), sheep (100), and goats (100) were inoculated into Mac Conkey Agar and incubated for 18 h at 37degree celsius. Escherichia coli isolates were confirmed by biochemical tests and the BBL Crystal rapid diagnosis system. O26, O45, O103, O111, O121, O145, and O157 serotypes by PCR test following DNA isolation; ETEC (elt, Stla); EPEC (eaeA,bfpA); STEC (stx1, stx2, eaeA); EHEC (EhlyA); EAEC (CVD432) tested for virulence and/or toxigenic genes. As a result of the isolation studies, 50 E. coli from cattle feces, 92 from sheep feces, and 80 from goat feces were isolated and identified. Apart from the first 5 serotypes frequently seen in studies (O157, O26, O103, O111, and O145), higher rates were found in serogroups such as O45 and O121, and subtypes such as STECs (stx1 and stx2), EPEC (eaeA and bfpA) and EAEC (CVD432) types compared to other studies. The EAEC (CVD432) subtype was found to be very high in this study. It has been determined that serotypes and subtypes detected at high rates in cattle, sheep, and goat feces in our region may cause an increase in the incidence of some critical food-borne infections in humans. Within the framework of the concept of one health, taking the necessary precautions is important for public health.
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    Mediating effect of work stress in the relationship between fear of COVID-19 and nurses' organizational and professional turnover intentions
    (W B Saunders Co-Elsevier Inc, 2023) Ekingen, Erhan; Teles, Mesut; Yildiz, Ahmet; Yildirim, Murat
    Nursing is one of the most stressful and high-risk professions. It is important to identify the psychological problems experienced by nurses during the COVID-19 pandemic and examine the relationship between these problems to devise measures that can properly address them. This study examined mediating effect of work stress in the relationship between fear of COVID-19 and nurses' organizational and professional turnover intentions. Using a cross-sectional research design, this study was conducted on 486 nurses working in seven hospitals in Turkey. The mean age of the participants was 35.24 +/- 6.81 and 59.9 % of them were women. The Fear of COVID-19 Scale, the General Work Stress Scale, and the Turnover Intention Scale were used to collect data. A mediation model showed that fear of COVID-19 was positively associated with work stress and organizational and pro-fessional turnover intentions. The model also revealed that work stress was positively associated with organi-zational and professional turnover intentions. Furthermore, the results demonstrated that fear of COVID-19 did not only have a direct effect on organizational and professional turnover intentions but also had an indirect effect on it via increased work stress. Findings improve our understanding of the role of work stress in the relationship between fear of COVID-19 and organizational and professional turnover intentions. The findings are fruitful for tailoring and implementing intervention programs to reduce the adverse psychological impacts of COVID-19 on nurses.