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    A new technique for preparing cadavers using PMMA: Rat and mouse example
    (Elsevier Gmbh, 2022) cinaroglu, Selim; Keles, Haci
    Background: Polymethyl methacrylate is a member of the acrylic polymer family with superior optical properties. It is used in dentistry, hospital incubators, lenses, and various surgical fields, especially ortho-pedics. In this preliminary study, we aimed to prepare cadavers using the industrial form of polymethyl methacrylate.Methods: The new technique consists of fixation, washing, purification, impregnation, and polymerization. For the study, two whole rat bodies, two rat front legs and two rat hind legs, one half rat body, two whole mouse bodies, and all internal organs were used. Wistar albino rats and BALB/c mice were used. After the cadaver samples were fixed and washed, they were passed through an alcohol series starting from 50%, completing the purification process. In the next step, a solution was prepared by mixing 100 ml of Thinner into 1000 ml of a mixture containing styrene, methyl methacrylate, and Co(II)2-ethyl hexanoate. The samples were immersed in the solution under negative pressure (from 10 mmHg to 400 mmHg), allowing the samples to absorb the solution thoroughly. They were then treated with methyl ethyl ketone peroxide and the polymerization step was terminated.Results: The cadaver samples obtained using polymer were hard, they maintained the given shape, and showed a relatively transparent quality. The production of these materials took 35 days, excluding fixation. The tissue samples were odorless in the process after polymerization. Also, there was no decay in the samples after the production stage and they could be stored under room conditions.Conclusion: This study determined that cadavers could be prepared using polymethyl methacrylate and methyl ethyl ketone peroxide. The polymer used here cost about $5. The cadavers can be safely exhibited in room conditions for five years without requiring special conditions like pools or cold storage. Further re-search should compare this technique with the plastination technique in terms of texture, color, weight, application time, and cost.(c) 2022 Elsevier GmbH. All rights reserved.