Hyaluronic acid enhances cell migration, viability, and mineralized tissue-specific genes in cementoblasts

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dc.authoridBozkurt, Serife Buket/0000-0002-8641-2844
dc.authoridBozic, Darko/0000-0002-0391-0574
dc.contributor.authorHakki, Sema S.
dc.contributor.authorBozkurt, Serife Buket
dc.contributor.authorSculean, Anton
dc.contributor.authorBozic, Darko
dc.date.accessioned2024-11-07T13:34:50Z
dc.date.available2024-11-07T13:34:50Z
dc.date.issued2024
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractBackground/Objectives: It has been repeatedly demonstrated that cementum formation is a crucial step in periodontal regeneration. Hyaluronic acid (HA) is an important component of the extracellular matrix which regulates cells functions and cell-cell communication. Hyaluronic acid/derivatives have been used in regenerative periodontal therapy, but the cellular effects of HA are still unknown. To investigate the effects of HA on cementoblast functions, cell viability, migration, mineralization, differentiation, and mineralized tissue-associated genes and cementoblast-specific markers of the cementoblasts were tested.Materials and Methods: Cementoblasts (OCCM-30) were treated with various dilutions (0, 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:128) of HA and examined for cell viability, migration, mineralization, and gene expressions. The mRNA expressions of osteocalcin (OCN), runt-related transcription factor 2 (Runx2), bone sialoprotein (BSP), collagen type I (COL-I), alkaline phosphatase (ALP), cementum protein-1 (CEMP-1), cementum attachment protein (CAP), and small mothers against decapentaplegic (Smad) -1, 2, 3, 6, 7, beta-catenin (Ctnnb1) were performed with real-time polymerase chain reaction (RT-PCR). Total RNA was isolated on days 3 and 8, and cell viability was determined using MTT assay on days 1 and 3. The cell mineralization was evaluated by von Kossa staining on day 8. Cell migration was assessed 2, 4, 6, and 24 hours following exposure to HA dilutions using an in vitro wound healing assay (0, 1:2, 1:4, 1:8).Results: At dilution of 1:2 to 1:128, HA importantly increased cell viability (p < .01). HA at a dilution of 1/2 increased wound healing rates after 4 h compared to the other dilutions and the untreated control group. Increased numbers of mineralized nodules were determined at dilutions of 1:2, 1:4, and 1:8 compared with control group. mRNA expressions of mineralized tissue marker including COL-I, BSP, RunX2, ALP, and OCN significantly improved by HA treatments compared with control group both on 3 days and on 8 days (p < .01). Smad 2, Smad 3, Smad 7, and beta-catenin (Ctnnb1) mRNAs were up-regulated, while Smad1 and Smad 6 were not affected by HA administration. Additionally, HA at dilutions of 1:2, 1:4, and 1:8 remarkably enhanced CEMP-1 and CAP expressions in a dilution- and time-dependent manner (p < .01).Conclusions: The present results have demonstrated that HA affected the expression of both mineralized tissue markers and cementoblast-specific genes. Positive effects of HA on the cementoblast functions demonstrated that HA application may play a key role in cementum regeneration.
dc.identifier.doi10.1111/jre.13201
dc.identifier.endpage73
dc.identifier.issn0022-3484
dc.identifier.issn1600-0765
dc.identifier.issue1
dc.identifier.pmid38069670
dc.identifier.scopus2-s2.0-85178911655
dc.identifier.scopusqualityQ1
dc.identifier.startpage63
dc.identifier.urihttps://doi.org/10.1111/jre.13201
dc.identifier.urihttps://hdl.handle.net/11480/16193
dc.identifier.volume59
dc.identifier.wosWOS:001118479500001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofJournal of Periodontal Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectcell viability
dc.subjectcementoblast
dc.subjecthyaluronic acid
dc.subjectmigration
dc.subjectmineralization
dc.subjectmRNA expression
dc.titleHyaluronic acid enhances cell migration, viability, and mineralized tissue-specific genes in cementoblasts
dc.typeArticle

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