Comparative metabolite profiling of drought stress in roots and leaves of seven Triticeae species

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dc.contributor.authorUllah N.
dc.contributor.authorYüce M.
dc.contributor.authorNeslihan Öztürk Gökçe Z.
dc.contributor.authorBudak H.
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2017
dc.departmentNiğde ÖHÜ
dc.description.abstractBackground: Drought is a lifestyle disease. Plant metabolomics has been exercised for understanding the fine-tuning of the potential pathways to surmount the adverse effects of drought stress. A broad spectrum of morphological and metabolic responses from seven Triticeae species including wild types with different drought tolerance/susceptibility level was investigated under control and water scarcity conditions. Results: Significant morphological parameters measured were root length, surface area, average root diameter and overall root development. Principal Component Analysis, Partial Least-Squares-Discriminant Analysis and Hierarchical Cluster Analysis were applied to the metabolomic data obtained by Gas Chromatography-Mass Spectrometry technique in order to determine the important metabolites of the drought tolerance across seven different Triticeae species. The metabolites showing significant accumulation under the drought stress were considered as the key metabolites and correlated with potential biochemical pathways, enzymes or gene locations for a better understanding of the tolerance mechanisms. In all tested species, 45 significantly active metabolites with possible roles in drought stress were identified. Twenty-one metabolites out of forty-five including sugars, amino acids, organic acids and low molecular weight compounds increased in both leaf and root samples of TR39477, IG132864 and Bolal under the drought stress, contrasting to TTD-22, Tosunbey, Ligustica and Meyeri samples. Three metabolites including succinate, aspartate and trehalose were selected for further genome analysis due to their increased levels in TR39477, IG132864, and Bolal upon drought stress treatment as well as their significant role in energy producing biochemical pathways. Conclusion: These results demonstrated that the genotypes with high drought tolerance skills, especially wild emmer wheat, have a great potential to be a genetic model system for experiments aiming to validate metabolomics-genomics networks. © 2017 The Author(s).
dc.description.sponsorshipThis research was funded by Sabanci University and Winifred-Asbjornson Plant Science Endowment.
dc.identifier.doi10.1186/s12864-017-4321-2
dc.identifier.issn1471-2164
dc.identifier.issue1
dc.identifier.pmid29246190
dc.identifier.scopus2-s2.0-85038095216
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://dx.doi.org/10.1186/s12864-017-4321-2
dc.identifier.urihttps://hdl.handle.net/11480/1762
dc.identifier.volume18
dc.identifier.wosWOS:000418046700002
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.institutionauthor[0-Belirlenecek]
dc.language.isoen
dc.publisherBioMed Central Ltd.
dc.relation.ispartofBMC Genomics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiochemical pathway
dc.subjectDrought stress
dc.subjectMetabolome
dc.subjectOrganic acids
dc.subjectPlant genomics
dc.subjectSystems biology
dc.subjectTriticeae
dc.titleComparative metabolite profiling of drought stress in roots and leaves of seven Triticeae species
dc.typeArticle

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