Dietary N-carbamylglutamate and L-arginine supplementation improves redox status and suppresses apoptosis in the colon of intrauterine growth-retarded suckling lambs

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dc.authoridMa, Yi/0000-0002-3781-314X
dc.authoridElsabagh, Mabrouk/0000-0002-0596-6547
dc.contributor.authorZhang, Hao
dc.contributor.authorZheng, Yi
dc.contributor.authorZha, Xia
dc.contributor.authorLiu, Xiaoyun
dc.contributor.authorMa, Yi
dc.contributor.authorLoor, Juan J.
dc.contributor.authorElsabagh, Mabrouk
dc.date.accessioned2024-11-07T13:35:07Z
dc.date.available2024-11-07T13:35:07Z
dc.date.issued2022
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractPrevious studies have revealed that dietary N-carbamylglutamate (NCG) or L-arginine (Arg) improves small intestinal integrity and immune function in suckling Hu lambs that have experienced intrauterine growth retardation (IUGR). Whether these nutrients alter redox status and apoptosis in the colon of IUGR lambs is still unknown. This study, therefore, aimed at investigating whether dietary supplementation of Arg or NCG alters colonic redox status, apoptosis and endoplasmic reticulum (ER) stress and the underlying mechanism of these alterations in IUGR suckling Hu lambs. Forty-eight 7-d old Hu lambs, including 12 with normal birth weight (4.25 +/- 0.14 kg) and 36 with IUGR (3.01 +/- 0.12 kg), were assigned to 4 treatment groups (n = 12 each; 6 males and 6 females) for 3 weeks. The treatment groups were control (CON), IUGR, IUGR + Arg and IUGR + NCG. Relative to IUGR lambs, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) content, as well as proliferation index, were higher (P < 0.05) whereas reactive oxygen species (ROS), malondialdehyde (MDA) levels and apoptotic cell numbers were lower (P < 0.05) in colonic tissue for both IUGR + Arg and NCG lambs. Both mRNA and protein levels of C/ EBP homologous protein 10 (CHOP10), B-cell lymphoma/leukaemia 2 (Bcl-2) -associated X protein (Bax), apoptosis antigen 1 (Fas), activating transcription factor 6 (ATF6), caspase 3, and glucose-regulated protein 78 (GRP78) were lower (P < 0.05) while glutathione peroxidase 1 (GPx1), Bcl-2 and catalase (CAT) levels were higher (P < 0.05) in colonic tissue for IUGR + Arg and IUGR + NCG lambs compared with IUGR lambs. Based on our results, dietary NCG or Arg supplementation can improve colonic redox status and suppress apoptosis via death receptor-dependent, mitochondrial and ER stress pathways in IUGR suckling lambs.(c) 2022 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/).
dc.description.sponsorshipNational Natural Science Foundation of China [31902180]; Top Talents Award Plan of Yangzhou University; Cyanine Project of Yangzhou University
dc.description.sponsorshipThis study was funded by the National Natural Science Foundation of China (31902180), the Top Talents Award Plan of Yangzhou University (2020), and the Cyanine Project of Yangzhou University (2020).
dc.identifier.doi10.1016/j.aninu.2022.08.009
dc.identifier.endpage368
dc.identifier.issn2405-6383
dc.identifier.issn2405-6545
dc.identifier.pmid36329684
dc.identifier.scopus2-s2.0-85140462128
dc.identifier.scopusqualityQ1
dc.identifier.startpage359
dc.identifier.urihttps://doi.org/10.1016/j.aninu.2022.08.009
dc.identifier.urihttps://hdl.handle.net/11480/16347
dc.identifier.volume11
dc.identifier.wosWOS:000890054400001
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherKeai Publishing Ltd
dc.relation.ispartofAnimal Nutrition
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectColon
dc.subjectEndoplasmic reticulum stress
dc.subjectIUGR lambs
dc.subjectL-arginine
dc.subjectRedox status
dc.titleDietary N-carbamylglutamate and L-arginine supplementation improves redox status and suppresses apoptosis in the colon of intrauterine growth-retarded suckling lambs
dc.typeArticle

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