Oxidative and apoptotic effects of fluoxetine and its metabolite norfluoxetine in Daphnia magna

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dc.authoridSevgiler, Yusuf/0000-0002-4373-2389
dc.contributor.authorOver, Sevgi Basalan
dc.contributor.authorGuven, Celal
dc.contributor.authorTaskin, Eylem
dc.contributor.authorSevgiler, Yusuf
dc.date.accessioned2024-11-07T13:32:02Z
dc.date.available2024-11-07T13:32:02Z
dc.date.issued2020
dc.departmentNiğde Ömer Halisdemir Üniversitesi
dc.description.abstractThe aim of this study was to investigate the oxidative and apoptotic potential of fluoxetine, a widely used antidepressant in Turkey and the world. and of its metabolite norfluoxetine on a model non-target organism, Daphnia magna to see how exposure to this group of antidepressants (specific serotonin reuptake inhibitors) could affect the aquatic environment in which they end up. Juvenile D. magna specimens were chronically exposed to fluoxetine and norfluoxetine alone and in combination at concentrations found in the aquatic environment (0.091 and 0.011 mu g/L, respectively) and to their 10-fold environmental concentrations for 21 days. Another group of 17-day-old animals were subacutely exposed to 100-fold environmental concentrations for four days. After exposure, we measured their glutathione peroxidase (GPx) and cholinesterase (ChE) activities, thiobarbituric acid-reactive substances (TBARS), and total protein content spectrophotometrically, while mitochondrial membrane potential (MMP) was analysed by fluorescence staining, and cytochrome c and ERK1/2 protein content by Western blotting. This is the first-time cytochrome c and ERK1/2 were determined at the protein level in D. magna. We also measured their carapace length, width. and caudal spine length microscopically. At environmental concentrations fluoxetine and norfluoxetine caused an increase in ChE activity and brood production. They also caused a decrease in juvenile carapace length, width, and caudal spine length and depolarised the mitochondrial membrane. At 10-fold environmental concentrations. GPx activity, lipid peroxidation levels. cytochrome c. and ERK1/2 protein levels rose. The most pronounced effect was observed in D. inagna exposed to norfluoxetine. Norfluoxetine also decreased brood production. Similar effects were observed with subacute exposure to 100-fold environmental concentrations. However, total protein content decreased. All this confirms that fluoxetine and norfluoxetine have oxidative and apoptotic potential in D. magna. Daphnia spp. have a great potential to give us precious insight into the mechanisms of environmental toxicants, but there is still a long way to go before they are clarified in these organisms.
dc.description.sponsorshipAdiyaman University Scientific Research Commission [FEFYL/2015-0003]
dc.description.sponsorshipWe would like to thank Dr Serdar Sonmez for his precious help in microscopic analysis. Adiyaman University Scientific Research Commission is gratefully acknowledged because of their support on this project (FEFYL/2015-0003).
dc.identifier.doi10.2478/aiht-2020-71-3473
dc.identifier.endpage222
dc.identifier.issn0004-1254
dc.identifier.issn1848-6312
dc.identifier.issue3
dc.identifier.pmid33074175
dc.identifier.scopus2-s2.0-85093986842
dc.identifier.scopusqualityQ3
dc.identifier.startpage211
dc.identifier.urihttps://doi.org/10.2478/aiht-2020-71-3473
dc.identifier.urihttps://hdl.handle.net/11480/15168
dc.identifier.volume71
dc.identifier.wosWOS:000580588500006
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherInst Medical Research & Occupational Health
dc.relation.ispartofArhiv Za Higijenu Rada I Toksikologiju-Archives of Industrial Hygiene and Toxicology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_20241106
dc.subjectcholinesterase
dc.subjectcytochrome c
dc.subjectERK1/2
dc.subjectGPx
dc.subjectlipid peroxidation
dc.subjectmitochondrial membrane potential
dc.subjectoxidative stress
dc.subjectTBARS
dc.titleOxidative and apoptotic effects of fluoxetine and its metabolite norfluoxetine in Daphnia magna
dc.typeArticle

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